Impedance measurements for detecting pathogens attached to antibodies
Abstract
The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.
- Inventors:
- Issue Date:
- Research Org.:
- The Regents of the Univ. of California, Oakland, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175183
- Patent Number(s):
- 6835552
- Application Number:
- 09/737,542
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Miles, Robin R., Venkateswaran, Kodumudi S., and Fuller, Christopher K. Impedance measurements for detecting pathogens attached to antibodies. United States: N. p., 2004.
Web.
Miles, Robin R., Venkateswaran, Kodumudi S., & Fuller, Christopher K. Impedance measurements for detecting pathogens attached to antibodies. United States.
Miles, Robin R., Venkateswaran, Kodumudi S., and Fuller, Christopher K. Tue .
"Impedance measurements for detecting pathogens attached to antibodies". United States. https://www.osti.gov/servlets/purl/1175183.
@article{osti_1175183,
title = {Impedance measurements for detecting pathogens attached to antibodies},
author = {Miles, Robin R. and Venkateswaran, Kodumudi S. and Fuller, Christopher K.},
abstractNote = {The use of impedance measurements to detect the presence of pathogens attached to antibody-coated beads. In a fluidic device antibodies are immobilized on a surface of a patterned interdigitated electrode. Pathogens in a sample fluid streaming past the electrode attach to the immobilized antibodies, which produces a change in impedance between two adjacent electrodes, which impedance change is measured and used to detect the presence of a pathogen. To amplify the signal, beads coated with antibodies are introduced and the beads would stick to the pathogen causing a greater change in impedance between the two adjacent electrodes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {12}
}