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Title: System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels

Abstract

A system and method for desorption and ionization of analytes in an ablation medium. In one embodiment, the method includes the steps of preparing a sample having analytes in a medium including at least one component, freezing the sample at a sufficiently low temperature so that at least part of the sample has a phase transition, and irradiating the frozen sample with short-pulse radiation to cause medium ablation and desorption and ionization of the analytes. The method further includes the steps of selecting a resonant vibrational mode of at least one component of the medium and selecting an energy source tuned to emit radiation substantially at the wavelength of the selected resonant vibrational mode. The medium is an electrophoresis medium having polyacrylamide. In one embodiment, the energy source is a laser, where the laser can be a free electron laser tunable to generate short-pulse radiation. Alternatively, the laser can be a solid state laser tunable to generate short-pulse radiation. The laser can emit light at various ranges of wavelength.

Inventors:
; ;
Issue Date:
Research Org.:
Vanderbilt Univ., Nashville, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175157
Patent Number(s):
6825045
Application Number:
09/931,490
Assignee:
Vanderbilt University
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY

Citation Formats

Haglund, Jr., Richard F., Ermer, David R., and Baltz-Knorr, Michelle Lee. System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels. United States: N. p., 2004. Web.
Haglund, Jr., Richard F., Ermer, David R., & Baltz-Knorr, Michelle Lee. System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels. United States.
Haglund, Jr., Richard F., Ermer, David R., and Baltz-Knorr, Michelle Lee. Tue . "System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels". United States. https://www.osti.gov/servlets/purl/1175157.
@article{osti_1175157,
title = {System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels},
author = {Haglund, Jr., Richard F. and Ermer, David R. and Baltz-Knorr, Michelle Lee},
abstractNote = {A system and method for desorption and ionization of analytes in an ablation medium. In one embodiment, the method includes the steps of preparing a sample having analytes in a medium including at least one component, freezing the sample at a sufficiently low temperature so that at least part of the sample has a phase transition, and irradiating the frozen sample with short-pulse radiation to cause medium ablation and desorption and ionization of the analytes. The method further includes the steps of selecting a resonant vibrational mode of at least one component of the medium and selecting an energy source tuned to emit radiation substantially at the wavelength of the selected resonant vibrational mode. The medium is an electrophoresis medium having polyacrylamide. In one embodiment, the energy source is a laser, where the laser can be a free electron laser tunable to generate short-pulse radiation. Alternatively, the laser can be a solid state laser tunable to generate short-pulse radiation. The laser can emit light at various ranges of wavelength.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}

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Works referenced in this record:

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  • Ermer, David R.; Papantonakis, Michael R.; Baltz-Knorr, Michelle
  • Symposium on High-Power Lasers and Applications, SPIE Proceedings
  • https://doi.org/10.1117/12.380797

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