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Title: Plant nitrogen regulatory P-PII polypeptides

Abstract

The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the invention may be used to engineer organisms to overexpress wild-type or mutant P-PII regulatory protein. Engineered plants that overexpress or underexpress P-PII regulatory protein may have increased nitrogen assimilation capacity. Engineered organisms may be used to produce P-PII proteins which, in turn, can be used for a variety of purposes including in vitro screening of herbicides. P-PII nucleotide sequences have additional uses as probes for isolating additional genomic clones having the promoters of P-PII gene. P-PII promoters are light- and/or sucrose-inducible and may be advantageously used in genetic engineering of plants.

Inventors:
; ;
Issue Date:
Research Org.:
New York University, New York, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175148
Patent Number(s):
6822079
Application Number:
09/756,541
Assignee:
New York University (New York, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER20133
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Coruzzi, Gloria M., Lam, Hon-Ming, and Hsieh, Ming-Hsiun. Plant nitrogen regulatory P-PII polypeptides. United States: N. p., 2004. Web.
Coruzzi, Gloria M., Lam, Hon-Ming, & Hsieh, Ming-Hsiun. Plant nitrogen regulatory P-PII polypeptides. United States.
Coruzzi, Gloria M., Lam, Hon-Ming, and Hsieh, Ming-Hsiun. Tue . "Plant nitrogen regulatory P-PII polypeptides". United States. https://www.osti.gov/servlets/purl/1175148.
@article{osti_1175148,
title = {Plant nitrogen regulatory P-PII polypeptides},
author = {Coruzzi, Gloria M. and Lam, Hon-Ming and Hsieh, Ming-Hsiun},
abstractNote = {The present invention generally relates to plant nitrogen regulatory PII gene (hereinafter P-PII gene), a gene involved in regulating plant nitrogen metabolism. The invention provides P-PII nucleotide sequences, expression constructs comprising said nucleotide sequences, and host cells and plants having said constructs and, optionally expressing the P-PII gene from said constructs. The invention also provides substantially pure P-PII proteins. The P-PII nucleotide sequences and constructs of the invention may be used to engineer organisms to overexpress wild-type or mutant P-PII regulatory protein. Engineered plants that overexpress or underexpress P-PII regulatory protein may have increased nitrogen assimilation capacity. Engineered organisms may be used to produce P-PII proteins which, in turn, can be used for a variety of purposes including in vitro screening of herbicides. P-PII nucleotide sequences have additional uses as probes for isolating additional genomic clones having the promoters of P-PII gene. P-PII promoters are light- and/or sucrose-inducible and may be advantageously used in genetic engineering of plants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}

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Works referenced in this record:

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