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Title: Method for chromium analysis and speciation

Abstract

A method of detecting a metal in a sample comprising a plurality of metal is disclosed. The method comprises providing the sample comprising a metal to be detected. The sample is added to a reagent solution comprising an enzyme and a substrate, where the enzyme is inhibited by the metal to be detected. An array of chelating agents is used to eliminate the inhibitory effects of additional metals in the sample. An enzymatic activity in the sample is determined and compared to an enzymatic activity in a control solution to detect the metal to be detected. A method of determining a concentration of the metal in the sample is also disclosed. A method of detecting a valence state of a metal is also disclosed.

Inventors:
; ; ;
Issue Date:
Research Org.:
Idaho National Lab. (INL), Idaho Falls, ID (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175109
Patent Number(s):
6811997
Application Number:
10/189,035
Assignee:
Bechtel BWXT Idaho, LLC (Idaho Falls, ID)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC07-99ID13727
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Aiken, Abigail M., Peyton, Brent M., Apel, William A., and Petersen, James N. Method for chromium analysis and speciation. United States: N. p., 2004. Web.
Aiken, Abigail M., Peyton, Brent M., Apel, William A., & Petersen, James N. Method for chromium analysis and speciation. United States.
Aiken, Abigail M., Peyton, Brent M., Apel, William A., and Petersen, James N. Tue . "Method for chromium analysis and speciation". United States. https://www.osti.gov/servlets/purl/1175109.
@article{osti_1175109,
title = {Method for chromium analysis and speciation},
author = {Aiken, Abigail M. and Peyton, Brent M. and Apel, William A. and Petersen, James N.},
abstractNote = {A method of detecting a metal in a sample comprising a plurality of metal is disclosed. The method comprises providing the sample comprising a metal to be detected. The sample is added to a reagent solution comprising an enzyme and a substrate, where the enzyme is inhibited by the metal to be detected. An array of chelating agents is used to eliminate the inhibitory effects of additional metals in the sample. An enzymatic activity in the sample is determined and compared to an enzymatic activity in a control solution to detect the metal to be detected. A method of determining a concentration of the metal in the sample is also disclosed. A method of detecting a valence state of a metal is also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {11}
}

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Works referenced in this record:

Phytochelatins protect plant enzymes from heavy metal poisoning
journal, August 1992


In vitro alleviation of heavy metal-induced enzyme inhibition by proline
journal, November 1998


A Spectrophotometric Assay for Nitrate Using NADPH Oxidation by Aspergillus Nitrate Reductase
journal, August 1993