Sample collection system for gel electrophoresis
Abstract
An automatic sample collection system for use with an electrophoretic slab gel system is presented. The collection system can be used with a slab gel have one or more lanes. A detector is used to detect particle bands on the slab gel within a detection zone. Such detectors may use a laser to excite fluorescently labeled particles. The fluorescent light emitted from the excited particles is transmitted to low-level light detection electronics. Upon the detection of a particle of interest within the detection zone, a syringe pump is activated, sending a stream of buffer solution across the lane of the slab gel. The buffer solution collects the sample of interest and carries it through a collection port into a sample collection vial.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175044
- Patent Number(s):
- 6793790
- Application Number:
- 09/875,333
- Assignee:
- University Of California, The Regents Of
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
Citation Formats
Olivares, Jose A., Stark, Peter C., Dunbar, John M., Hill, Karen K., Kuske, Cheryl R., and Roybal, Gustavo. Sample collection system for gel electrophoresis. United States: N. p., 2004.
Web.
Olivares, Jose A., Stark, Peter C., Dunbar, John M., Hill, Karen K., Kuske, Cheryl R., & Roybal, Gustavo. Sample collection system for gel electrophoresis. United States.
Olivares, Jose A., Stark, Peter C., Dunbar, John M., Hill, Karen K., Kuske, Cheryl R., and Roybal, Gustavo. Tue .
"Sample collection system for gel electrophoresis". United States. https://www.osti.gov/servlets/purl/1175044.
@article{osti_1175044,
title = {Sample collection system for gel electrophoresis},
author = {Olivares, Jose A. and Stark, Peter C. and Dunbar, John M. and Hill, Karen K. and Kuske, Cheryl R. and Roybal, Gustavo},
abstractNote = {An automatic sample collection system for use with an electrophoretic slab gel system is presented. The collection system can be used with a slab gel have one or more lanes. A detector is used to detect particle bands on the slab gel within a detection zone. Such detectors may use a laser to excite fluorescently labeled particles. The fluorescent light emitted from the excited particles is transmitted to low-level light detection electronics. Upon the detection of a particle of interest within the detection zone, a syringe pump is activated, sending a stream of buffer solution across the lane of the slab gel. The buffer solution collects the sample of interest and carries it through a collection port into a sample collection vial.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 21 00:00:00 EDT 2004},
month = {Tue Sep 21 00:00:00 EDT 2004}
}
Works referenced in this record:
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journal, January 2000
- Irie, Takashi; Oshida, Tadahiro; Hasegawa, Hideki
- Electrophoresis, Vol. 21, Issue 2, p. 367-374
Preparative Electrophoresis in a Concentrated Polymer Solution: Automated Procedure for Microsome Isolation
journal, April 1997
- Chang, Huan-Tsung; Chen, Nong; Sullivan, James V.
- Analytical Biochemistry, Vol. 247, Issue 1