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Title: Method for assaying clustered DNA damages

Abstract

Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.

Inventors:
Issue Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175022
Patent Number(s):
6789022
Application Number:
09/837,560
Assignee:
Brookhaven Science Associates LLC (Upton, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC02-98CH10886
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Sutherland, Betsy M. Method for assaying clustered DNA damages. United States: N. p., 2004. Web.
Sutherland, Betsy M. Method for assaying clustered DNA damages. United States.
Sutherland, Betsy M. Tue . "Method for assaying clustered DNA damages". United States. https://www.osti.gov/servlets/purl/1175022.
@article{osti_1175022,
title = {Method for assaying clustered DNA damages},
author = {Sutherland, Betsy M.},
abstractNote = {Disclosed is a method for detecting and quantifying clustered damages in DNA. In this method, a first aliquot of the DNA to be tested for clustered damages with one or more lesion-specific cleaving reagents under conditions appropriate for cleavage of the DNA to produce single-strand nicks in the DNA at sites of damage lesions. The number average molecular length (Ln) of double stranded DNA is then quantitatively determined for the treated DNA. The number average molecular length (Ln) of double stranded DNA is also quantitatively determined for a second, untreated aliquot of the DNA. The frequency of clustered damages (.PHI..sub.c) in the DNA is then calculated.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {9}
}

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Works referenced in this record:

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journal, July 1996


Reactivity of Human Apurinic/Apyrimidinic Endonuclease and Escherichia coli Exonuclease III with Bistranded Abasic Sites in DNA
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Fluorescent labelling of abasic sites: a novel methodology to detect closely-spaced damage sites in DNA
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Yield of DNA Strand Breaks after Base Oxidation of Plasmid DNA
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Biochemistry of DNA Lesions
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The Role of DNA Single- and Double-Strand Breaks in Cell Killing by Ionizing Radiation
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