Chemical inducible promoter used to obtain transgenic plants with a silent marker
Abstract
A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.
- Inventors:
- Issue Date:
- Research Org.:
- The Rockefeller University, New York, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175006
- Patent Number(s):
- 6784340
- Application Number:
- 09/438,392
- Assignee:
- The Rockefeller University (New York, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-94ER20142
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Aoyama, Takashi, Zuo, Jianru, and Chua, Nam-Hai. Chemical inducible promoter used to obtain transgenic plants with a silent marker. United States: N. p., 2004.
Web.
Aoyama, Takashi, Zuo, Jianru, & Chua, Nam-Hai. Chemical inducible promoter used to obtain transgenic plants with a silent marker. United States.
Aoyama, Takashi, Zuo, Jianru, and Chua, Nam-Hai. Tue .
"Chemical inducible promoter used to obtain transgenic plants with a silent marker". United States. https://www.osti.gov/servlets/purl/1175006.
@article{osti_1175006,
title = {Chemical inducible promoter used to obtain transgenic plants with a silent marker},
author = {Aoyama, Takashi and Zuo, Jianru and Chua, Nam-Hai},
abstractNote = {A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {8}
}
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