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Title: Chemical inducible promoter used to obtain transgenic plants with a silent marker

Abstract

A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.

Inventors:
; ;
Issue Date:
Research Org.:
The Rockefeller University, New York, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175006
Patent Number(s):
6784340
Application Number:
09/438,392
Assignee:
The Rockefeller University (New York, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER20142
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Aoyama, Takashi, Zuo, Jianru, and Chua, Nam-Hai. Chemical inducible promoter used to obtain transgenic plants with a silent marker. United States: N. p., 2004. Web.
Aoyama, Takashi, Zuo, Jianru, & Chua, Nam-Hai. Chemical inducible promoter used to obtain transgenic plants with a silent marker. United States.
Aoyama, Takashi, Zuo, Jianru, and Chua, Nam-Hai. Tue . "Chemical inducible promoter used to obtain transgenic plants with a silent marker". United States. https://www.osti.gov/servlets/purl/1175006.
@article{osti_1175006,
title = {Chemical inducible promoter used to obtain transgenic plants with a silent marker},
author = {Aoyama, Takashi and Zuo, Jianru and Chua, Nam-Hai},
abstractNote = {A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {8}
}

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