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Title: Rolling circle amplification detection of RNA and DNA

Abstract

Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175004
Patent Number(s):
6783943
Application Number:
10/032,017
Assignee:
University Of California, The Regents Of
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Christian, Allen T., Pattee, Melissa S., Attix, Cristina M., and Tucker, James D. Rolling circle amplification detection of RNA and DNA. United States: N. p., 2004. Web.
Christian, Allen T., Pattee, Melissa S., Attix, Cristina M., & Tucker, James D. Rolling circle amplification detection of RNA and DNA. United States.
Christian, Allen T., Pattee, Melissa S., Attix, Cristina M., and Tucker, James D. Tue . "Rolling circle amplification detection of RNA and DNA". United States. https://www.osti.gov/servlets/purl/1175004.
@article{osti_1175004,
title = {Rolling circle amplification detection of RNA and DNA},
author = {Christian, Allen T. and Pattee, Melissa S. and Attix, Cristina M. and Tucker, James D.},
abstractNote = {Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {8}
}

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Works referenced in this record:

Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells
journal, November 2001