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Title: Cast-to-shape electrokinetic trapping medium

Abstract

A three-dimensional microporous polymer network material, or monolith, cast-to-shape in a microchannel. The polymer monolith, produced by a phase separation process, is capable of trapping and retaining charged protein species from a mixture of charged and uncharged species under the influence of an applied electric field. The retained charged protein species are released from the porous polymer monolith by a pressure driven flow in the substantial absence of the electric field. The pressure driven flow is independent of direction and thus neither means to reverse fluid flow nor a multi-directional flow field is required, a single flow through the porous polymer monolith can be employed, in contrast to prior art systems. The monolithic polymer material produced by the invention can function as a chromatographic medium. Moreover, by virtue of its ability to retain charged protein species and quantitatively release the retained species the porous polymer monolith can serve as a means for concentrating charged protein species from, for example, a dilute solution.

Inventors:
; ; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174969
Patent Number(s):
6770201
Application Number:
10/213,842
Assignee:
Sandia National Laboratories (Livermore, CA)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE

Citation Formats

Shepodd, Timothy J., Franklin, Elizabeth, Prickett, Zane T., and Artau, Alexander. Cast-to-shape electrokinetic trapping medium. United States: N. p., 2004. Web.
Shepodd, Timothy J., Franklin, Elizabeth, Prickett, Zane T., & Artau, Alexander. Cast-to-shape electrokinetic trapping medium. United States.
Shepodd, Timothy J., Franklin, Elizabeth, Prickett, Zane T., and Artau, Alexander. Tue . "Cast-to-shape electrokinetic trapping medium". United States. https://www.osti.gov/servlets/purl/1174969.
@article{osti_1174969,
title = {Cast-to-shape electrokinetic trapping medium},
author = {Shepodd, Timothy J. and Franklin, Elizabeth and Prickett, Zane T. and Artau, Alexander},
abstractNote = {A three-dimensional microporous polymer network material, or monolith, cast-to-shape in a microchannel. The polymer monolith, produced by a phase separation process, is capable of trapping and retaining charged protein species from a mixture of charged and uncharged species under the influence of an applied electric field. The retained charged protein species are released from the porous polymer monolith by a pressure driven flow in the substantial absence of the electric field. The pressure driven flow is independent of direction and thus neither means to reverse fluid flow nor a multi-directional flow field is required, a single flow through the porous polymer monolith can be employed, in contrast to prior art systems. The monolithic polymer material produced by the invention can function as a chromatographic medium. Moreover, by virtue of its ability to retain charged protein species and quantitatively release the retained species the porous polymer monolith can serve as a means for concentrating charged protein species from, for example, a dilute solution.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {8}
}

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Works referenced in this record:

Strategy for On-Line Preconcentration in Chromatographic Separations
journal, November 2001


On-Line Preconcentration in Capillary Electrochromatography Using a Porous Monolith Together with Solvent Gradient and Sample Stacking
journal, November 2001


Photopolymerized Sol−Gel Monoliths for Capillary Electrochromatography
journal, August 2001