Microfabricated capillary array electrophoresis device and method
Abstract
A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174894
- Patent Number(s):
- 6749734
- Application Number:
- 09/649,272
- Assignee:
- University Of California, The Regents Of
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- FG-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING
Citation Formats
Simpson, Peter C., Mathies, Richard A., and Woolley, Adam T. Microfabricated capillary array electrophoresis device and method. United States: N. p., 2004.
Web.
Simpson, Peter C., Mathies, Richard A., & Woolley, Adam T. Microfabricated capillary array electrophoresis device and method. United States.
Simpson, Peter C., Mathies, Richard A., and Woolley, Adam T. Tue .
"Microfabricated capillary array electrophoresis device and method". United States. https://www.osti.gov/servlets/purl/1174894.
@article{osti_1174894,
title = {Microfabricated capillary array electrophoresis device and method},
author = {Simpson, Peter C. and Mathies, Richard A. and Woolley, Adam T.},
abstractNote = {A capillary array electrophoresis (CAE) micro-plate with an array of separation channels connected to an array of sample reservoirs on the plate. The sample reservoirs are organized into one or more sample injectors. One or more waste reservoirs are provided to collect wastes from reservoirs in each of the sample injectors. Additionally, a cathode reservoir is also multiplexed with one or more separation channels. To complete the electrical path, an anode reservoir which is common to some or all separation channels is also provided on the micro-plate. Moreover, the channel layout keeps the distance from the anode to each of the cathodes approximately constant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {6}
}
Works referenced in this record:
Fluorescence scanner employing a macro scanning objective
conference, March 1996
- Alexay, Christopher C.; Kain, Robert C.; Hanzel, David K.
- Photonics West '96, SPIE Proceedings
High-throughput genetic analysis using microfabricated 96-sample capillary array electrophoresis microplates
journal, March 1998
- Simpson, P. C.; Roach, D.; Woolley, A. T.
- Proceedings of the National Academy of Sciences, Vol. 95, Issue 5
Planar chips technology for miniaturization and integration of separation techniques into monitoring systems
journal, February 1992
- Manz, Andreas; Harrison, D. Jed; Verpoorte, Elisabeth M. J.
- Journal of Chromatography A, Vol. 593, Issue 1-2
Simultaneous monitoring of DNA fragments separated by electrophoresis in a multiplexed array of 100 capillaries
journal, April 1994
- Ueno, Kyoji.; Yeung, Edward S.
- Analytical Chemistry, Vol. 66, Issue 9
High-speed separations of DNA sequencing reactions by capillary electrophoresis
journal, May 1990
- Drossman, Howard.; Luckey, John A.; Kostichka, Anthony J.
- Analytical Chemistry, Vol. 62, Issue 9
A novel MHC class I–like gene is mutated in patients with hereditary haemochromatosis
journal, August 1996
- Feder, J. N.; Gnirke, A.; Thomas, W.
- Nature Genetics, Vol. 13, Issue 4
Energy-transfer fluorescent reagents for DNA analyses
journal, February 1997
- Glazer, Alexander N.; Mathies, Richard A.
- Current Opinion in Biotechnology, Vol. 8, Issue 1
High-performance electrophoresis
journal, January 1985
- Hjertén, Stellan
- Journal of Chromatography A, Vol. 347
Ultra-High-Speed DNA Sequencing Using Capillary Electrophoresis Chips
journal, October 1995
- Woolley, Adam T.; Mathies, Richard A.
- Analytical Chemistry, Vol. 67, Issue 20
Functional Integration of PCR Amplification and Capillary Electrophoresis in a Microfabricated DNA Analysis Device
journal, January 1996
- Woolley, Adam T.; Hadley, Dean; Landre, Phoebe
- Analytical Chemistry, Vol. 68, Issue 23
Multiple Sheath-Flow Gel Capillary-Array Electrophoresis for Multicolor Fluorescent DNA Detection
journal, April 1994
- Takahashi, Satoshi.; Murakami, Katsuhiko.; Anazawa, Takashi.
- Analytical Chemistry, Vol. 66, Issue 7
Capillary electrophoresis and sample injection systems integrated on a planar glass chip
journal, September 1992
- Harrison, D. Jed.; Manz, Andreas.; Fan, Zhonghui.
- Analytical Chemistry, Vol. 64, Issue 17
Separation and analysis of DNA sequence reaction products by capillary gel electrophoresis
journal, September 1990
- Cohen, A. S.; Najarian, D. R.; Karger, B. L.
- Journal of Chromatography A, Vol. 516, Issue 1
Accessing Genetic Information with High-Density DNA Arrays
journal, October 1996
- Chee, M.; Yang, R.; Hubbell, E.
- Science, Vol. 274, Issue 5287
Microsatellite-based cancer detection using capillary array electrophoresis and energy-transfer fluorescent primers
journal, January 1997
- Wang, Yiwen; Hung, Su-Chun; Linn, Jürgen F.
- Electrophoresis, Vol. 18, Issue 10
Glass chips for high-speed capillary electrophoresis separations with submicrometer plate heights
journal, October 1993
- Effenhauser, Carlo S.; Manz, Andreas.; Widmer, H. Michael.
- Analytical Chemistry, Vol. 65, Issue 19
High-Speed Separation of Antisense Oligonucleotides on a Micromachined Capillary Electrophoresis Device
journal, September 1994
- Effenhauser, Carlo S.; Paulus, Aran.; Manz, Andreas.
- Analytical Chemistry, Vol. 66, Issue 18
Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips.
journal, November 1994
- Woolley, A. T.; Mathies, R. A.
- Proceedings of the National Academy of Sciences, Vol. 91, Issue 24
Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects
journal, April 1997
- Li, Paul C. H.; Harrison, D. Jed
- Analytical Chemistry, Vol. 69, Issue 8
DNA sequencing using capillary array electrophoresis
journal, September 1992
- Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A.
- Analytical Chemistry, Vol. 64, Issue 18
A simple salting out procedure for extracting DNA from human nucleated cells
journal, January 1988
- Miller, S. A.; Dykes, D. D.; Polesky, H. F.
- Nucleic Acids Research, Vol. 16, Issue 3
High Speed Automated DNA Sequencing in Ultrathin Slab Gels
journal, January 1992
- Kostichka, Anthony J.; Marchbanks, Michael L.; Brumley, Robert L.
- Nature Biotechnology, Vol. 10, Issue 1
DNA sequencing using a four-color confocal fluorescence capillary array scanner
journal, January 1996
- Kheterpal, Indu; Scherer, James R.; Clark, Steven M.
- Electrophoresis, Vol. 17, Issue 12, p. 1852-1859
Capillary array electrophoresis using laser-excited confocal fluorescence detection
journal, April 1992
- Huang, Xiaohua C.; Quesada, Mark A.; Mathies, Richard A.
- Analytical Chemistry, Vol. 64, Issue 8
Microchip Capillary Electrophoresis with an Integrated Postcolumn Reactor
journal, October 1994
- Jacobson, Stephen C.; Koutny, Lance B.; Hergenroeder, Roland.
- Analytical Chemistry, Vol. 66, Issue 20
Integrated Microdevice for DNA Restriction Fragment Analysis
journal, January 1996
- Jacobson, Stephen C.; Ramsey, J. Michael
- Analytical Chemistry, Vol. 68, Issue 5
Multiplex dsDNA Fragment Sizing Using Dimeric Intercalation Dyes and Capillary Array Electrophoresis: Ionic Effects on the Stability and Electrophoretic Mobility of DNA−Dye Complexes
journal, April 1997
- Clark, Steven M.; Mathies, Richard A.
- Analytical Chemistry, Vol. 69, Issue 7
High-resolution capillary array electrophoretic sizing of multiplexed short tandem repeat loci using energy-transfer fluorescent primers
journal, January 1996
- Wang, Yiwen; Wallin, Jeanette M.; Ju, Jingyue
- Electrophoresis, Vol. 17, Issue 9
Capillary gel electrophoresis for rapid, high resolution DNA sequencing
journal, January 1990
- Swerdlow, Harold; Gesteland, Raymond
- Nucleic Acids Research, Vol. 18, Issue 6
High-Speed Parallel Separation of DNA Restriction Fragments Using Capillary Array Electrophoresis
journal, December 1993
- Clark, S. M.; Mathies, R. A.
- Analytical Biochemistry, Vol. 215, Issue 2
Micromachining a Miniaturized Capillary Electrophoresis-Based Chemical Analysis System on a Chip
journal, August 1993
- Harrison, D. J.; Fluri, K.; Seiler, K.
- Science, Vol. 261, Issue 5123
Global prevalence of putative haemochromatosis mutations.
journal, April 1997
- Merryweather-Clarke, A. T.; Pointon, J. J.; Shearman, J. D.
- Journal of Medical Genetics, Vol. 34, Issue 4