Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
Abstract
A method for rapid and efficient detection of a target DNA or RNA sequence is provided. A primer having a 3'-hydroxyl group at one end and having a sequence of nucleotides sufficiently homologous with an identifying sequence of nucleotides in the target DNA is selected. The primer is hybridized to the identifying sequence of nucleotides on the DNA or RNA sequence and a reporter molecule is synthesized on the target sequence by progressively binding complementary nucleotides to the primer, where the complementary nucleotides include nucleotides labeled with a fluorophore. Fluorescence emitted by fluorophores on single reporter molecules is detected to identify the target DNA or RNA sequence.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174871
- Patent Number(s):
- 6743578
- Application Number:
- 09/454,385
- Assignee:
- University Of California, The Regents Of
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Castro, Alonso. Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation. United States: N. p., 2004.
Web.
Castro, Alonso. Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation. United States.
Castro, Alonso. Tue .
"Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation". United States. https://www.osti.gov/servlets/purl/1174871.
@article{osti_1174871,
title = {Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation},
author = {Castro, Alonso},
abstractNote = {A method for rapid and efficient detection of a target DNA or RNA sequence is provided. A primer having a 3'-hydroxyl group at one end and having a sequence of nucleotides sufficiently homologous with an identifying sequence of nucleotides in the target DNA is selected. The primer is hybridized to the identifying sequence of nucleotides on the DNA or RNA sequence and a reporter molecule is synthesized on the target sequence by progressively binding complementary nucleotides to the primer, where the complementary nucleotides include nucleotides labeled with a fluorophore. Fluorescence emitted by fluorophores on single reporter molecules is detected to identify the target DNA or RNA sequence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {6}
}
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Works referenced in this record:
Single-Molecule Detection of Specific Nucleic Acid Sequences in Unamplified Genomic DNA
journal, October 1997
- Castro, Alonso; Williams, John G. K.
- Analytical Chemistry, Vol. 69, Issue 19
In situ transcription with Tth DNA polymerase and fluorescent nucleotides
journal, December 1994
- Chang, Henry
- Journal of Immunological Methods, Vol. 176, Issue 2
Single-Molecule electrophoresis
journal, September 1995
- Castro, Alonso.; Shera, E. Brooks.
- Analytical Chemistry, Vol. 67, Issue 18