Title: Method and apparatus for detection of fluorescently labeled materials

Abstract

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

Inventors:

Stern, David; Fiekowsky, Peter

Issue Date:

2004-05-25

Research Org.:

Affymetrix, Inc., Santa Clara, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1174867

Patent Number(s):

6741344

Application Number:

09/699,852

Assignee:

Affymetrix, Inc. (Santa Clara, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/00605 - the compounds being directly bound or immobilised to solid supports
B01J2219/00612 - the surface being inorganic
B01J2219/00626 - Covalent
B01J2219/00659 - Two-dimensional arrays
B01J2219/00702 - Processes involving means for analysing and characterising the products

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N2021/0346 - {Capillary cells
G01N21/05 - Flow-through cuvettes
G01N21/6428 - {Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
G01N21/645 - {Specially adapted constructive features of fluorimeters}
G01N21/6452 - {Individual samples arranged in a regular 2D-array, e.g. multiwell plates}
G01N21/6456 - {Spatial resolved fluorescence measurements
G01N21/6458 - {Fluorescence microscopy

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DOE Contract Number:  

FG03-92ER81275

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

36 MATERIALS SCIENCE