Engineering of living cells for the expression of holo-phycobiliprotein-based constructs
Abstract
Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174863
- Patent Number(s):
- 6740507
- Application Number:
- 09/919,486
- Assignee:
- University Of California, The Regents Of
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG-9161125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs. United States: N. p., 2004.
Web.
Glazer, Alexander N., Tooley, Aaron J., & Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs. United States.
Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Tue .
"Engineering of living cells for the expression of holo-phycobiliprotein-based constructs". United States. https://www.osti.gov/servlets/purl/1174863.
@article{osti_1174863,
title = {Engineering of living cells for the expression of holo-phycobiliprotein-based constructs},
author = {Glazer, Alexander N. and Tooley, Aaron J. and Cai, Yuping},
abstractNote = {Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue May 25 00:00:00 EDT 2004},
month = {Tue May 25 00:00:00 EDT 2004}
}
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Works referenced in this record:
Functional Genomic Analysis of the HY2 Family of Ferredoxin-Dependent Bilin Reductases from Oxygenic Photosynthetic Organisms
journal, April 2001
- Frankenberg, Nicole; Mukougawa, Keiko; Kohchi, Takayuki
- The Plant Cell, Vol. 13, Issue 4
Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo- subunit in a heterologous host
journal, September 2001
- Tooley, A. J.; Cai, Y. A.; Glazer, A. N.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 19
Genetic engineering of phytochrome biosynthesis in bacteria
journal, September 2001
- Gambetta, G. A.; Lagarias, J. C.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 19
Recombinant holophytochrome in Escherichia coli
journal, November 2001
- Landgraf, F. T.; Forreiter, C.; Hurtado Picó, A.
- FEBS Letters, Vol. 508, Issue 3
Phycocyanin alpha-subunit phycocyanobilin lyase.
journal, August 1992
- Fairchild, C. D.; Zhao, J.; Zhou, J.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 15
The use of phycobiliproteins as fluorescent labels in immunoassay
journal, August 1986
- Kronick, Mel N.
- Journal of Immunological Methods, Vol. 92, Issue 1