skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

Abstract

Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174863
Patent Number(s):
6740507
Application Number:
09/919,486
Assignee:
University Of California, The Regents Of
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG-9161125
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs. United States: N. p., 2004. Web.
Glazer, Alexander N., Tooley, Aaron J., & Cai, Yuping. Engineering of living cells for the expression of holo-phycobiliprotein-based constructs. United States.
Glazer, Alexander N., Tooley, Aaron J., and Cai, Yuping. Tue . "Engineering of living cells for the expression of holo-phycobiliprotein-based constructs". United States. https://www.osti.gov/servlets/purl/1174863.
@article{osti_1174863,
title = {Engineering of living cells for the expression of holo-phycobiliprotein-based constructs},
author = {Glazer, Alexander N. and Tooley, Aaron J. and Cai, Yuping},
abstractNote = {Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

Functional Genomic Analysis of the HY2 Family of Ferredoxin-Dependent Bilin Reductases from Oxygenic Photosynthetic Organisms
journal, April 2001


Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-  subunit in a heterologous host
journal, September 2001


Genetic engineering of phytochrome biosynthesis in bacteria
journal, September 2001


Recombinant holophytochrome in Escherichia coli
journal, November 2001


Phycocyanin alpha-subunit phycocyanobilin lyase.
journal, August 1992


The use of phycobiliproteins as fluorescent labels in immunoassay
journal, August 1986