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Title: Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction

Abstract

A method, compositions and kit are set forth for detecting blood stains. A reactant solution includes fluorescin solubilized (reduced) in acetic acid in ethanol. The solution may be buffered to a pH of approximately 9. After spraying the reactant solution on the suspected area an oxidizer is applied to promote the fluorescin to fluorescein reaction with the blood. The reacted fluorescein is then detected through luminescence for capture by photography.

Inventors:
; ; ;
Issue Date:
Research Org.:
~Individually Owned Patent
Sponsoring Org.:
USDOE
OSTI Identifier:
1174732
Patent Number(s):
6692967
Application Number:
09/894,301
Assignee:
Di Benedetto, John; Kyle, Kevin; Boan, Terry; Marie, Charlene
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC08-96NV11718
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Di Benedetto, John, Kyle, Kevin, Boan, Terry, and Marie, Charlene. Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction. United States: N. p., 2004. Web.
Di Benedetto, John, Kyle, Kevin, Boan, Terry, & Marie, Charlene. Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction. United States.
Di Benedetto, John, Kyle, Kevin, Boan, Terry, and Marie, Charlene. Tue . "Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction". United States. https://www.osti.gov/servlets/purl/1174732.
@article{osti_1174732,
title = {Method and compositions for detecting of bloodstains using fluorescin-fluorescein reaction},
author = {Di Benedetto, John and Kyle, Kevin and Boan, Terry and Marie, Charlene},
abstractNote = {A method, compositions and kit are set forth for detecting blood stains. A reactant solution includes fluorescin solubilized (reduced) in acetic acid in ethanol. The solution may be buffered to a pH of approximately 9. After spraying the reactant solution on the suspected area an oxidizer is applied to promote the fluorescin to fluorescein reaction with the blood. The reacted fluorescein is then detected through luminescence for capture by photography.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {2}
}

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Works referenced in this record:

Hyperconjugation in hydrocarbons: Not just a “mild sort of conjugation”
journal, April 2013