Use of CYP52A2A promoter to increase gene expression in yeast
Abstract
A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.
- Inventors:
- Issue Date:
- Research Org.:
- Cognis Corporation, Gulph Mills, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174665
- Patent Number(s):
- 6673613
- Application Number:
- 09/911,781
- Assignee:
- Cognis Corporation (Gulph Mills, PA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FC36-95GO10099
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Craft, David L., Wilson, C. Ron, Eirich, Dudley, and Zhang, Yeyan. Use of CYP52A2A promoter to increase gene expression in yeast. United States: N. p., 2004.
Web.
Craft, David L., Wilson, C. Ron, Eirich, Dudley, & Zhang, Yeyan. Use of CYP52A2A promoter to increase gene expression in yeast. United States.
Craft, David L., Wilson, C. Ron, Eirich, Dudley, and Zhang, Yeyan. Tue .
"Use of CYP52A2A promoter to increase gene expression in yeast". United States. https://www.osti.gov/servlets/purl/1174665.
@article{osti_1174665,
title = {Use of CYP52A2A promoter to increase gene expression in yeast},
author = {Craft, David L. and Wilson, C. Ron and Eirich, Dudley and Zhang, Yeyan},
abstractNote = {A nucleic acid sequence including a CYP promoter operably linked to nucleic acid encoding a heterologous protein is provided to increase transcription of the nucleic acid. Expression vectors and host cells containing the nucleic acid sequence are also provided. The methods and compositions described herein are especially useful in the production of polycarboxylic acids by yeast cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {1}
}
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Works referenced in this record:
Characterization of a second alkane-inducible cytochrome P450-encoding gene, CYP52A2, from Candida tropicalis
journal, September 1991
- Seghezzi, Wolfgang; Sanglard, Dominique; Fiechter, Armin
- Gene, Vol. 106, Issue 1