Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display
Abstract
The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174586
- Patent Number(s):
- 6649376
- Application Number:
- 09/469,194
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- DOE Contract Number:
- FG-91ER61125
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Glazer, Alexander N., and Cai, Yuping. Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display. United States: N. p., 2003.
Web.
Glazer, Alexander N., & Cai, Yuping. Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display. United States.
Glazer, Alexander N., and Cai, Yuping. Tue .
"Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display". United States. https://www.osti.gov/servlets/purl/1174586.
@article{osti_1174586,
title = {Multifunctional recombinant phycobiliprotein-based fluorescent constructs and phycobilisome display},
author = {Glazer, Alexander N. and Cai, Yuping},
abstractNote = {The invention provides multifunctional fusion constructs which are rapidly incorporated into a macromolecular structure such as a phycobilisome such that the fusion proteins are separated from one another and unable to self-associate. The invention provides methods and compositions for displaying a functional polypeptide domain on an oligomeric phycobiliprotein, including fusion proteins comprising a functional displayed domain and a functional phycobiliprotein domain incorporated in a functional oligomeric phycobiliprotein. The fusion proteins provide novel specific labeling reagents.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {11}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Recombinant Phycobiliproteins
journal, March 2001
- Cai, Yuping A.; Murphy, John T.; Wedemayer, Gary J.
- Analytical Biochemistry, Vol. 290, Issue 2
Heterologous assembly and rescue of stranded phycocyanin subunits by expression of a foreign cpcBA operon in Synechocystis sp. strain 6803.
journal, January 1995
- Plank, T.; Anderson, L. K.
- Journal of bacteriology, Vol. 177, Issue 23
Detection of Dimers of Dimers of Human Leukocyte Antigen (HLA)–DR on the Surface of Living Cells by Single-Particle Fluorescence Imaging
journal, January 1998
- Cherry, Richard J.; Wilson, Keith M.; Triantafilou, Kathy
- Journal of Cell Biology, Vol. 140, Issue 1