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Title: PCR amplification on microarrays of gel immobilized oligonucleotides

Abstract

The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174559
Patent Number(s):
6,642,000
Application Number:
09/559,306
Assignee:
University Of Chicago OSTI
DOE Contract Number:  
W-31-1-0-ENG-39
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 42 ENGINEERING

Citation Formats

Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States: N. p., 2003. Web.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, & Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. Tue . "PCR amplification on microarrays of gel immobilized oligonucleotides". United States. https://www.osti.gov/servlets/purl/1174559.
@article{osti_1174559,
title = {PCR amplification on microarrays of gel immobilized oligonucleotides},
author = {Strizhkov, Boris and Tillib, Sergei and Mikhailovich, Vladimir and Mirzabekov, Andrei},
abstractNote = {The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {11}
}

Patent:

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