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Title: PCR amplification on microarrays of gel immobilized oligonucleotides

Abstract

The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.

Inventors:
; ; ;
Issue Date:
Research Org.:
Univ. of Chicago, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174559
Patent Number(s):
6642000
Application Number:
09/559,306
Assignee:
University Of Chicago
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-31-1-0-ENG-39
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; 42 ENGINEERING

Citation Formats

Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States: N. p., 2003. Web.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, & Mirzabekov, Andrei. PCR amplification on microarrays of gel immobilized oligonucleotides. United States.
Strizhkov, Boris, Tillib, Sergei, Mikhailovich, Vladimir, and Mirzabekov, Andrei. Tue . "PCR amplification on microarrays of gel immobilized oligonucleotides". United States. https://www.osti.gov/servlets/purl/1174559.
@article{osti_1174559,
title = {PCR amplification on microarrays of gel immobilized oligonucleotides},
author = {Strizhkov, Boris and Tillib, Sergei and Mikhailovich, Vladimir and Mirzabekov, Andrei},
abstractNote = {The invention relates two general methods for performing PCR amplification, combined with the detection and analysis of the PCR products on a microchip. In the first method, the amplification occurs both outside and within a plurality of gel pads on a microchip, with at least one oligonucleotide primer immobilized in a gel pad. In the second method, PCR amplification also takes place within gel pads on a microchip, but the pads are surrounded by a hydrophobic liquid such as that which separates the individual gel pads into environments which resemble micro-miniaturized test tubes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {11}
}

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Works referenced in this record:

Immobilization of DNA in Polyacrylamide Gel for the Manufacture of DNA and DNA–Oligonucleotide Microchips
journal, May 1998


Fractionation, phosphorylation and ligation on oligonucleotide microchips to enhance sequencing by hybridization
journal, June 1997


Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips
journal, March 1998


Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip
journal, December 1998


Protein Microchips: Use for Immunoassay and Enzymatic Reactions
journal, February 2000


DNA analysis and diagnostics on oligonucleotide microchips.
journal, May 1996