skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Manipulation of lignin composition in plants using a tissue-specific promoter

Abstract

The present invention relates to methods and materials in the field of molecular biology, the manipulation of the phenylpropanoid pathway and the regulation of proteins synthesis through plant genetic engineering. More particularly, the invention relates to the introduction of a foreign nucleotide sequence into a plant genome, wherein the introduction of the nucleotide sequence effects an increase in the syringyl content of the plant's lignin. In one specific aspect, the invention relates to methods for modifying the plant lignin composition in a plant cell by the introduction there into of a foreign nucleotide sequence comprising at issue specific plant promoter sequence and a sequence encoding an active ferulate-5-hydroxylase (F5H) enzyme. Plant transformants harboring an inventive promoter-F5H construct demonstrate increased levels of syringyl monomer residues in their lignin, rendering the polymer more readily delignified and, thereby, rendering the plant more readily pulped or digested.

Inventors:
Issue Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174465
Patent Number(s):
6610908
Application Number:
09/214,139
Assignee:
Purdue Research Foundation (West Lafayette, IN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER20138
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chapple, Clinton C. S. Manipulation of lignin composition in plants using a tissue-specific promoter. United States: N. p., 2003. Web.
Chapple, Clinton C. S. Manipulation of lignin composition in plants using a tissue-specific promoter. United States.
Chapple, Clinton C. S. Tue . "Manipulation of lignin composition in plants using a tissue-specific promoter". United States. https://www.osti.gov/servlets/purl/1174465.
@article{osti_1174465,
title = {Manipulation of lignin composition in plants using a tissue-specific promoter},
author = {Chapple, Clinton C. S.},
abstractNote = {The present invention relates to methods and materials in the field of molecular biology, the manipulation of the phenylpropanoid pathway and the regulation of proteins synthesis through plant genetic engineering. More particularly, the invention relates to the introduction of a foreign nucleotide sequence into a plant genome, wherein the introduction of the nucleotide sequence effects an increase in the syringyl content of the plant's lignin. In one specific aspect, the invention relates to methods for modifying the plant lignin composition in a plant cell by the introduction there into of a foreign nucleotide sequence comprising at issue specific plant promoter sequence and a sequence encoding an active ferulate-5-hydroxylase (F5H) enzyme. Plant transformants harboring an inventive promoter-F5H construct demonstrate increased levels of syringyl monomer residues in their lignin, rendering the polymer more readily delignified and, thereby, rendering the plant more readily pulped or digested.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {8}
}

Patent:

Save / Share:

Works referenced in this record:

Ferulate-5-hydroxylase from Arabidopsis thaliana defines a new family of cytochrome P450-dependent monooxygenases.
journal, July 1996


Purification and characterization of isoforms of cinnamyl alcohol dehydrogenase fromEucalyptus xylem
journal, January 1992


Manipulation of lignin quality by downregulation of cinnamyl alcohol dehydrogenase
journal, September 1994


Cinnamate-4-Hydroxylase Expression in Arabidopsis (Regulation in Response to Development and the Environment)
journal, March 1997


Phenylpropanoid metabolism and lignin biosynthesis: from weeds to trees
journal, June 1996


Lignin genetic engineering
journal, January 1996


Modification of lignin biosynthesis in transgenic Nicotiana through expression of an antisense O-methyltransferase gene from Populus
journal, October 1994


Red Xylem and Higher Lignin Extractability by Down-Regulating a Cinnamyl Alcohol Dehydrogenase in Poplar
journal, December 1996


Reduced lignin in transgenic plants containing a caffeic acidO-methyltransferase antisense gene
journal, March 1994


An Arabidopsis mutant defective in the general phenylpropanoid pathway.
journal, November 1992