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Title: Real time viability detection of bacterial spores

Abstract

This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.

Inventors:
; ;
Issue Date:
Research Org.:
University Of California, The Regents Of
Sponsoring Org.:
USDOE
OSTI Identifier:
1174421
Patent Number(s):
6,599,715
Application Number:
09/570,137
Assignee:
The Regents of the University of California (Los Alamos, NM) OSTI
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Real time viability detection of bacterial spores. United States: N. p., 2003. Web.
Vanderberg, Laura A., Herdendorf, Timothy J., & Obiso, Richard J. Real time viability detection of bacterial spores. United States.
Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Tue . "Real time viability detection of bacterial spores". United States. https://www.osti.gov/servlets/purl/1174421.
@article{osti_1174421,
title = {Real time viability detection of bacterial spores},
author = {Vanderberg, Laura A. and Herdendorf, Timothy J. and Obiso, Richard J.},
abstractNote = {This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {7}
}

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