Real time viability detection of bacterial spores
Abstract
This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of California, Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1174421
- Patent Number(s):
- 6599715
- Application Number:
- 09/570,137
- Assignee:
- The Regents of the University of California (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Real time viability detection of bacterial spores. United States: N. p., 2003.
Web.
Vanderberg, Laura A., Herdendorf, Timothy J., & Obiso, Richard J. Real time viability detection of bacterial spores. United States.
Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Tue .
"Real time viability detection of bacterial spores". United States. https://www.osti.gov/servlets/purl/1174421.
@article{osti_1174421,
title = {Real time viability detection of bacterial spores},
author = {Vanderberg, Laura A. and Herdendorf, Timothy J. and Obiso, Richard J.},
abstractNote = {This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jul 29 00:00:00 EDT 2003},
month = {Tue Jul 29 00:00:00 EDT 2003}
}
Works referenced in this record:
Dissociation of Spore Germination from Outgrowth by use of Auxotrophic Mutants of Bacillus Subtilis
journal, January 1960
- Demain, Arnold L.; Newkirk, Joanne F.
- Journal of Bacteriology, Vol. 79, Issue 6
Bacterial Spore Detection and Determination by Use of Terbium Dipicolinate Photoluminescence
journal, March 1997
- Rosen, David L.; Sharpless, Charles; McGown, Linda B.
- Analytical Chemistry, Vol. 69, Issue 6
Intensely Luminescent Immunoreactive Conjugates of Proteins and Dipicolinate-Based Polymeric Tb(III) Chelates
journal, January 1995
- Lamture, Jagannath B.; Wensel, Theodore G.
- Bioconjugate Chemistry, Vol. 6, Issue 1
Chemical germination of native and cation-exchanged bacterial spores with trifluoperazine.
journal, January 1990
- Sacks, L. E.
- Applied and Environmental Microbiology, Vol. 56, Issue 4