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Title: Real time viability detection of bacterial spores

Abstract

This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.

Inventors:
; ;
Issue Date:
Research Org.:
University Of California, The Regents Of
Sponsoring Org.:
USDOE
OSTI Identifier:
1174421
Patent Number(s):
6599715
Application Number:
09/570,137
Assignee:
The Regents of the University of California (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Real time viability detection of bacterial spores. United States: N. p., 2003. Web.
Vanderberg, Laura A., Herdendorf, Timothy J., & Obiso, Richard J. Real time viability detection of bacterial spores. United States.
Vanderberg, Laura A., Herdendorf, Timothy J., and Obiso, Richard J. Tue . "Real time viability detection of bacterial spores". United States. https://www.osti.gov/servlets/purl/1174421.
@article{osti_1174421,
title = {Real time viability detection of bacterial spores},
author = {Vanderberg, Laura A. and Herdendorf, Timothy J. and Obiso, Richard J.},
abstractNote = {This invention relates to a process for detecting the presence of viable bacterial spores in a sample and to a spore detection system, the process including placing a sample in a germination medium for a period of time sufficient for commitment of any present viable bacterial spores to occur, mixing the sample with a solution of a lanthanide capable of forming a fluorescent complex with dipicolinic acid, and, measuring the sample for the presence of dipicolinic acid, and the system including a germination chamber having inlets from a sample chamber, a germinant chamber and a bleach chamber, the germination chamber further including an outlet through a filtering means, the outlet connected to a detection chamber, the detection chamber having an inlet from a fluorescence promoting metal chamber and the detection chamber including a spectral excitation source and a means of measuring emission spectra from a sample, the detection chamber further connected to a waste chamber. A germination reaction mixture useful for promoting commitment of any viable bacterial spores in a sample including a combination of L-alanine, L-asparagine and D-glucose is also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {7}
}

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Works referenced in this record:

Dissociation of Spore Germination from Outgrowth by use of Auxotrophic Mutants of Bacillus Subtilis
journal, January 1960


Bacterial Spore Detection and Determination by Use of Terbium Dipicolinate Photoluminescence
journal, March 1997


Intensely Luminescent Immunoreactive Conjugates of Proteins and Dipicolinate-Based Polymeric Tb(III) Chelates
journal, January 1995


Chemical germination of native and cation-exchanged bacterial spores with trifluoperazine.
journal, January 1990