DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods and compositions for chromosome-specific staining


Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.

Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Country of Publication:
United States

Citation Formats

Gray, Joe W., and Pinkel, Daniel. Methods and compositions for chromosome-specific staining. United States: N. p., 2003. Web.
Gray, Joe W., & Pinkel, Daniel. Methods and compositions for chromosome-specific staining. United States.
Gray, Joe W., and Pinkel, Daniel. Tue . "Methods and compositions for chromosome-specific staining". United States.
title = {Methods and compositions for chromosome-specific staining},
author = {Gray, Joe W. and Pinkel, Daniel},
abstractNote = {Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {7}

Works referenced in this record:

Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.
journal, March 1986

Isolation and characterization of a major tandem repeat family from the human X chromosome
journal, January 1983

Detection of species specific chromosomes in somatic cell hybrids
journal, November 1985

Partial purification and characterization of DNA from the human X chromosome.
journal, December 1979

Detection of the Philadelphia chromosome in interphase nuclei
journal, January 1990

A central role for chromosome breakage in gene amplification, deletion formation, and amplicon integration.
journal, February 1991

[38] Nucleic acid hybridization in cytological preparations
book, January 1971

Four restriction fragment length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q
journal, January 1986

Autoradiographic Detection of Molecular Hybrids between rRNA and DNA in Tissue Sections
journal, March 1970

Quantitative in situ hybridization of ribosomal RNA species to polytene chromosomes of Drosophila melanogaster
journal, September 1977

What's new with hybridization in situ?
journal, January 1982

ERBB2 amplification in breast cancer analyzed by fluorescence in situ hybridization.
journal, June 1992

Fetal cells in the blood of pregnant women: detection and enrichment by fluorescence-activated cell sorting.
journal, March 1979

RNA-DNA Hybrids at the Cytological Level
journal, August 1969

Cytological Hybridization to Mammalian Chromosomes
book, January 1982

Localization of the human insulin gene to the distal end of the short arm of chromosome 11.
journal, July 1981

Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes
journal, April 1981

Localization of single copy DNA sequences on G-banded human chromosomes by in situ hybridization
journal, July 1981

Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization
journal, August 1986

Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
journal, May 1986

Organization and heterogeneity of sequences within a repeating unit of human Y chromosome deoxyribonucleic acid
journal, July 1979

Isolation of unique sequence human X chromosomal deoxyribonucleic acid
journal, May 1980

Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization
journal, December 1987

A highly polymorphic locus on chromosome 16q revealed by a probe from a chromosome-specific cosmid library
journal, December 1986

Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization
journal, October 1989

Detection of DNA sequences in nuclei in suspension by in situ hybridization and dual beam flow cytometry
journal, December 1985

Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis
journal, October 1987

Regional assignment of genes for human esterase D and retinoblastoma to chromosome band 13q14
journal, May 1980

Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.
journal, June 1984

Hereditary Renal-Cell Carcinoma Associated with a Chromosomal Translocation
journal, September 1979

Formation and Detection of Rna-Dna Hybrid Molecules in Cytological Preparations
journal, June 1969

Chromosomal and nuclear distribution of the HindIII 1.9-kb human DNA repeat segment
journal, December 1984

Human retinoblastoma susceptibility gene: genomic organization and analysis of heterozygous intragenic deletion mutants.
journal, April 1988

Different central nervous system cell types display distinct and nonrandom arrangements of satellite DNA sequences.
journal, May 1984

Characterization of a cloned DNA sequence that is present at centromeres of all human autosomes and the X chromosome and shows polymorphic variation.
journal, August 1984

DNA diagnostics--molecular techniques and automation
journal, October 1988

Construction of fifteen human chromosome-specific DNA libraries from flow-purified chromosomes
journal, January 1986

Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.
journal, October 1990

Detection of amplified dna sequences in human tumor cell lines by fluorescence in situ hybridization
journal, June 1992

A procedure for the isolation of deoxyribonucleic acid from micro-organisms
journal, April 1961

Fluorescence in situ hybridization with Alu and L1 polymerase chain reaction probes for rapid characterization of human chromosomes in hybrid cell lines.
journal, September 1990

Genomic mismatch scanning: a new approach to genetic linkage mapping
journal, May 1993

Report of the committee on structural chromosome changes in neoplasia
journal, January 1989

Hereditary spherocytosis associated with deletion of human erythrocyte ankyrin gene on chromosome 8
journal, June 1990

General method for cloning amplified DNA by differential screening with genomic probes.
journal, May 1982

The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit β-globin DNA
journal, January 1981

Two homoeo box loci mapped in evolutionarily related mouse and human chromosomes
journal, March 1985

Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.
journal, October 1975

Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.
journal, December 1988

Rapid detection of human chromosome 21 aberrations by in situ hybridization.
journal, December 1988

In situ hybridization and translocation breakpoint mapping
journal, January 1985

Optimizing comparative genomic hybridization for analysis of DNA sequence copy number changes in solid tumors
journal, August 1994

Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila
journal, May 1981

Detection of structural and numerical abnormalities in metaphase spreads and interphase nuclei using in situ hybridization
journal, September 1989

Organization and evolution of the class I gene family in the major histocompatibility complex of the C57BL/10 mouse
journal, August 1984

Removal of repeated sequences from hybridisation probes
journal, January 1985

A human DNA segment with properties of the gene that predisposes to retinoblastoma and osteosarcoma
journal, October 1986

Characterization of chromosomal anomalies in human breast cancer
journal, October 1990

Directional cloning of DNA fragments at a large distance from an initial probe: a circularization method.
journal, November 1984

The Use of the Avidin-Biotin Complex as a Tool in Molecular Biology
book, February 1980

Adhesive and degradative properties of human placental cytotrophoblast cells in vitro.
journal, August 1989

Immunological method for mapping genes on Drosophila polytene chromosomes.
journal, July 1982

Is non-isotopic in situ hybridization finally coming of age?
journal, May 1990

The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNA
journal, December 1978

In Situ Hybridization to Cellular RNAs
book, January 1985

Proto-oncogene amplification and homogeneously staining regions in human breast carcinomas
journal, May 1990

Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes
journal, January 1977

Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization
journal, September 1985

Electron microscopic visualization of tRNA genes with ferritin-avidin: biotin labels
journal, January 1978

Cloned DNA probes regionally mapped to human Chromosome 21 and their use in determining the origin of nondisjunction
journal, January 1985

Structure of amplified DNA in different Syrian hamster cell lines resistant to N-(phosphonacetyl)-L-aspartate.
journal, November 1983

Comparative genomic hybridization for molecular cytogenetic analysis of solid tumors
journal, October 1992

Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy.
journal, February 1992

Assignment of the structural gene coding for albumin to human chromosome 4
journal, January 1982

Distinctive chromosomal structures are formed very early in the amplification of CAD genes in Syrian hamster cells
journal, December 1990

Clinical Applications of DNA Probes in the Diagnosis of Genetic Diseases
journal, January 1987

Localization of a unique gene by direct hybridization in situ.
journal, June 1981

Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes
journal, February 1984

Individual interphase chromosome domains revealed by in situ hybridization
journal, December 1985

Quantitation of in situ hybridization of ribosomal ribonucleic acids to human diploid cells
journal, October 1980

Organization and chromosomal specificity of autosomal homologs of human Y chromosome repeated DNA
journal, January 1985

A polymorphic locus on the long arm of chromosome 20 defined by two probes from a single cosmid
journal, August 1986

Cytogenetic Analysis by In Situ Hybridization with Fluorescently Labeled Nucleic Acid Probes
journal, January 1986

2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences
journal, July 1984

A highly polymorphic locus in human DNA revealed by cosmid-derived probes.
journal, September 1985

Biotin and fluorescent labeling of RNA using T4 RNA ligase
journal, January 1983

High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones
journal, January 1990

Construction and characterization of genomic libraries from specific human chromosomes.
journal, May 1982