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Title: Injector-concentrator electrodes for microchannel electrophoresis

Abstract

An input port geometry, with injector-concentrator electrodes, for planar microchannel array for electrophoresis. This input port geometry enables efficient extraction and injection of the DNA sample from a single input port. The geometry, which utilizes injector-concentrator electrodes, allows simultaneous concentration, in different channels, of the sample into a longitudinally narrow strip just before releasing it for a run with enhanced injection spatial resolution, and time resolution. Optional multiple electrodes, at a different bias than the concentrator electrodes, may be used to discriminate against sample impurity ions. Electrode passivation can be utilized to prevent electrolysis. An additional electrode in or on the input hole can better define the initial loading. The injector-concentrator electrodes are positioned so that they cross the drift channel in a narrow strip at the bond plane between the top and bottom plates of the instrument and are located close to the inlet hole. The optional sample purification electrodes are located at a greater distance from the input hole than the injector-concentrate electrodes.

Inventors:
Issue Date:
Research Org.:
University of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1174304
Patent Number(s):
6,558,523
Application Number:
09/545,616
Assignee:
The Regents of the University of California (Oakland, CA) OSTI
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Swierkowski, Stefan P. Injector-concentrator electrodes for microchannel electrophoresis. United States: N. p., 2003. Web.
Swierkowski, Stefan P. Injector-concentrator electrodes for microchannel electrophoresis. United States.
Swierkowski, Stefan P. Tue . "Injector-concentrator electrodes for microchannel electrophoresis". United States. https://www.osti.gov/servlets/purl/1174304.
@article{osti_1174304,
title = {Injector-concentrator electrodes for microchannel electrophoresis},
author = {Swierkowski, Stefan P.},
abstractNote = {An input port geometry, with injector-concentrator electrodes, for planar microchannel array for electrophoresis. This input port geometry enables efficient extraction and injection of the DNA sample from a single input port. The geometry, which utilizes injector-concentrator electrodes, allows simultaneous concentration, in different channels, of the sample into a longitudinally narrow strip just before releasing it for a run with enhanced injection spatial resolution, and time resolution. Optional multiple electrodes, at a different bias than the concentrator electrodes, may be used to discriminate against sample impurity ions. Electrode passivation can be utilized to prevent electrolysis. An additional electrode in or on the input hole can better define the initial loading. The injector-concentrator electrodes are positioned so that they cross the drift channel in a narrow strip at the bond plane between the top and bottom plates of the instrument and are located close to the inlet hole. The optional sample purification electrodes are located at a greater distance from the input hole than the injector-concentrate electrodes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {5}
}

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