Nucleic acid detection system and method for detecting influenza
Abstract
The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.
- Inventors:
- Issue Date:
- Research Org.:
- Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1172753
- Patent Number(s):
- 8,980,561
- Application Number:
- 11/894,908
- Assignee:
- Los Alamos National Security, LLC. (Los Alamos, NM)
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Aug 22
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Cai, Hong, and Song, Jian. Nucleic acid detection system and method for detecting influenza. United States: N. p., 2015.
Web.
Cai, Hong, & Song, Jian. Nucleic acid detection system and method for detecting influenza. United States.
Cai, Hong, and Song, Jian. Tue .
"Nucleic acid detection system and method for detecting influenza". United States. https://www.osti.gov/servlets/purl/1172753.
@article{osti_1172753,
title = {Nucleic acid detection system and method for detecting influenza},
author = {Cai, Hong and Song, Jian},
abstractNote = {The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {3}
}
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