Nucleic acid detection system and method for detecting influenza

Cai, Hong; Song, Jian

Title: Nucleic acid detection system and method for detecting influenza

Abstract

The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.

Inventors:: Cai, Hong; Song, Jian

Issue Date:: 2015-03-17

Research Org.:: Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1172753

Patent Number(s):: 8980561

Application Number:: 11/894,908

Assignee:: Los Alamos National Security, LLC. (Los Alamos, NM)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/6853 - using modified primers or templates
C12Q2600/158 - Expression markers
C12Q2600/16 - Primer sets for multiplex assays
C12Q1/6816 - characterised by the detection means
C12Q1/701 - {Specific hybridization probes}

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DOE Contract Number:: AC52-06NA25396

Resource Type:: Patent

Resource Relation:: Patent File Date: 2007 Aug 22

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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                    Cai, Hong, and Song, Jian. Nucleic acid detection system and method for detecting influenza.  United States: N. p., 2015. 
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                    Cai, Hong, & Song, Jian. Nucleic acid detection system and method for detecting influenza.  United States.

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                    Cai, Hong, and Song, Jian. Tue .  
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@article{osti_1172753,

  title        = {Nucleic acid detection system and method for detecting influenza},

  author       = {Cai, Hong and Song, Jian},

  abstractNote = {The invention provides a rapid, sensitive and specific nucleic acid detection system which utilizes isothermal nucleic acid amplification in combination with a lateral flow chromatographic device, or DNA dipstick, for DNA-hybridization detection. The system of the invention requires no complex instrumentation or electronic hardware, and provides a low cost nucleic acid detection system suitable for highly sensitive pathogen detection. Hybridization to single-stranded DNA amplification products using the system of the invention provides a sensitive and specific means by which assays can be multiplexed for the detection of multiple target sequences.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {3}

}

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Masny, A.
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Title: Nucleic acid detection system and method for detecting influenza

Abstract

Citation Formats

Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods journal, February 1999

Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses journal, December 1997

An array of opportunities journal, April 2002

Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification journal, March 1994

Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes journal, April 1988

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification journal, June 2005

Current Protocols in Molecular Biology book, May 2001

A rapid biosensor for viable B. anthracis spores journal, August 2004

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits journal, February 2004

Biosensor for Dengue Virus Detection: Sensitive, Rapid, and Serotype Specific journal, March 2002

Biosensors for environmental pollutants and food contaminants journal, October 2003

The ligase chain reaction in a PCR world. journal, August 1991

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns journal, April 1996

Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood journal, May 2006

Thermodynamic effects of formamide on DNA stability journal, June 1996

Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA journal, January 2001

Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern journal, October 2005

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans journal, November 2002

Shape matters journal, March 2003

Signal amplification through nucleotide extension and excision on a dendritic DNA platform journal, April 2000

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography journal, April 2007

Helicase structure and mechanism journal, February 2002

Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa journal, September 1993

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick journal, July 2000

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip journal, December 2001

Lab-on-a-chip devices for global health: Past studies and future opportunities journal, January 2007

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers journal, March 2004

Multiplex diagnostic platforms for detection of biothreat agents journal, November 2004

Purification and characterization of Thermus thermophilus UvrD journal, February 2003

Nucleic acid sequence-based amplification journal, March 1991

Synthesis and hybridization of a series of biotinylated oligonucleotides journal, January 1988

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR journal, March 2002

Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker journal, June 2001

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium journal, January 1978

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray journal, January 2007

Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency journal, September 1991

Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection journal, December 2005

Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA) journal, January 2002

Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay journal, August 2005

RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase journal, May 1979

A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis journal, December 1996

Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis journal, April 2005

Specific detection of Bacillus anthracis using aTaqMan® mismatch amplification mutation assay journal, May 2005

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization journal, August 2006

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides journal, October 2003

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum journal, October 2004

Potential Impact of Antiviral Drug Use during Influenza Pandemic journal, September 2005

An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA journal, July 2000

Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus journal, August 2003

Detection of foodborne pathogens using DNA probes and a dipstick format journal, December 1996

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. journal, March 1990

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports journal, January 1994

Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase journal, October 2000

Biosensor for the specific detection of a single viable B. anthracis spore journal, May 2003

Rapid and Sensitive Colorimetric Detection of Xanthomonas axonopodis pv. citri by Immunocapture and a Nested-Polymerase Chain Reaction Assay journal, January 1996

DNA Microarray Technology Devices, Systems, and Applications journal, August 2002

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa journal, February 2001

Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector journal, December 1997

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates journal, February 2004

Xylella Fastidiosa: Xylem-Limited Bacterial Pathogen of Plants journal, September 1989

Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays journal, April 2002

Point-of-care diagnostics: will the hurdles be overcome this time? journal, July 2006

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia journal, October 2005

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses journal, October 1998

Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture journal, April 2004

Encoded evidence: DNA in forensic analysis journal, October 2004

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides journal, January 1995

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence journal, March 1999

Fidelity of DNA polymerases in DNA amplification. journal, December 1989

NASBATM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection journal, December 1991

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic journal, July 2002

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays journal, August 2006

Technology platforms for pharmacogenomic diagnostic assays journal, September 2004

Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA journal, February 1999

Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay journal, July 2000

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal, February 1989

A ligase-mediated gene detection technique journal, August 1988

Recovery and detection of enterovirus, hepatitis A virus and Norwalk virus in hardshell clams (Mercenaria mercenaria) by RT-PCR methods
journal, February 1999

Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses
journal, December 1997

An array of opportunities
journal, April 2002

Identification of the Heme Compound Copurified with Deoxyribonucleic Acid (DNA) from Bloodstains, a Major Inhibitor of Polymerase Chain Reaction (PCR) Amplification
journal, March 1994

Optimal conditions for hybridization with oligonucleotides: A study with myc-oncogene DNA probes
journal, April 1988

Characterization of a Thermostable UvrD Helicase and Its Participation in Helicase-dependent Amplification
journal, June 2005

Current Protocols in Molecular Biology
book, May 2001

A rapid biosensor for viable B. anthracis spores
journal, August 2004

A Universal Nucleic Acid Sequence Biosensor with Nanomolar Detection Limits
journal, February 2004

Biosensor for Dengue Virus Detection: Sensitive, Rapid, and Serotype Specific
journal, March 2002

Biosensors for environmental pollutants and food contaminants
journal, October 2003

The ligase chain reaction in a PCR world.
journal, August 1991

Rapid, direct extraction of DNA from soils for PCR analysis using polyvinylpolypyrrolidone spin columns
journal, April 1996

Rapid, Sensitive, and Specific Lateral-Flow Immunochromatographic Device To Measure Anti-Anthrax Protective Antigen Immunoglobulin G in Serum and Whole Blood
journal, May 2006

Thermodynamic effects of formamide on DNA stability
journal, June 1996

Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA
journal, January 2001

Incidence of adamantane resistance among influenza A (H3N2) viruses isolated worldwide from 1994 to 2005: a cause for concern
journal, October 2005

Transmission of the Citrus Variegated Chlorosis Bacterium Xylella fastidiosa with the Sharpshooter Oncometopia nigricans
journal, November 2002

Shape matters
journal, March 2003

Signal amplification through nucleotide extension and excision on a dendritic DNA platform
journal, April 2000

Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography
journal, April 2007

Helicase structure and mechanism
journal, February 2002

Culture and serological detection of the xylem-limited bacterium causing citrus variegated chlorosis and its identification as a strain ofXylella fastidiosa
journal, September 1993

Early diagnosis of bubonic plague using F1 antigen capture ELISA assay and rapid immunogold dipstick
journal, July 2000

Chemiluminescence Detection for Hybridization Assays on the Flow-Thru Chip, a Three-Dimensional Microchannel Biochip
journal, December 2001

Lab-on-a-chip devices for global health: Past studies and future opportunities
journal, January 2007

A nested-PCR assay for detection of Xylella fastidiosa in citrus plants and sharpshooter leafhoppers
journal, March 2004

Multiplex diagnostic platforms for detection of biothreat agents
journal, November 2004

Purification and characterization of Thermus thermophilus UvrD
journal, February 2003

Nucleic acid sequence-based amplification
journal, March 1991

Synthesis and hybridization of a series of biotinylated oligonucleotides
journal, January 1988

Genetic Relationship among Worldwide Strains of Xanthomonas Causing Canker in Citrus Species and Design of New Primers for Their Identification by PCR
journal, March 2002

Quantitative PCR Method for Diagnosis of Citrus Bacterial Canker
journal, June 2001

Pierce's Disease of Grapevines: Isolation of the Causal Bacterium
journal, January 1978

Identification of A/H5N1 Influenza Viruses Using a Single Gene Diagnostic Microarray
journal, January 2007

Immobilization of polynucleotides on magnetic particles. Factors influencing hybridization efficiency
journal, September 1991

Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection
journal, December 2005

Characteristics and Applications of Nucleic Acid Sequence-Based Amplification (NASBA)
journal, January 2002

Simultaneous Visual Detection of Multiple Viral Amplicons by Dipstick Assay
journal, August 2005

RNA replication: required intermediates and the dissociation of template, product, and Q.beta. replicase
journal, May 1979

A coupled complex of T4 DNA replication helicase (gp41) and polymerase (gp43) can perform rapid and processive DNA strand-displacement synthesis
journal, December 1996

Use of Single Nucleotide Polymorphisms in the plcR Gene for Specific Identification of Bacillus anthracis
journal, April 2005

Specific detection of Bacillus anthracis using aTaqMan® mismatch amplification mutation assay
journal, May 2005

Optimization of DNA-tagged dye-encapsulating liposomes for lateral-flow assays based on sandwich hybridization
journal, August 2006

Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides
journal, October 2003

Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum
journal, October 2004

Potential Impact of Antiviral Drug Use during Influenza Pandemic
journal, September 2005

An investigation of the rigor of interpretation rules for STRs derived from less than 100 pg of DNA
journal, July 2000

Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus
journal, August 2003

Detection of foodborne pathogens using DNA probes and a dipstick format
journal, December 1996

Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
journal, March 1990

Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports
journal, January 1994

Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase
journal, October 2000

Biosensor for the specific detection of a single viable B. anthracis spore
journal, May 2003

Rapid and Sensitive Colorimetric Detection of Xanthomonas axonopodis pv. citri by Immunocapture and a Nested-Polymerase Chain Reaction Assay
journal, January 1996

DNA Microarray Technology Devices, Systems, and Applications
journal, August 2002

Genetic Diversity of Pierce's Disease Strains and Other Pathotypes of Xylella fastidiosa
journal, February 2001

Populations of Xylella fastidiosa in Plants Required for Transmission by an Efficient Vector
journal, December 1997

Fluorescent Amplified Fragment Length Polymorphism Analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis Isolates
journal, February 2004

Xylella Fastidiosa: Xylem-Limited Bacterial Pathogen of Plants
journal, September 1989

Accessing Single Nucleotide Polymorphisms in Genomic DNA by Direct Multiplex Polymerase Chain Reaction Amplification on Oligonucleotide Microarrays
journal, April 2002

Point-of-care diagnostics: will the hurdles be overcome this time?
journal, July 2006

Detection of amantadine-resistant variants among avian influenza viruses isolated in North America and Asia
journal, October 2005

Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and tobacco mosaic tobamoviruses
journal, October 1998

Direct isolation of poly(A)+ RNA from 4 M guanidine thiocyanate-lysed cell extracts using locked nucleic acid-oligo(T) capture
journal, April 2004

Encoded evidence: DNA in forensic analysis
journal, October 2004

Design, biochemical, biophysical and biological properties of cooperative antisense oligonucleotides
journal, January 1995

DnaB from Thermus aquaticus Unwinds Forked Duplex DNA with an Asymmetric Tail Length Dependence
journal, March 1999

Fidelity of DNA polymerases in DNA amplification.
journal, December 1989

NASBA^TM isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection
journal, December 1991

The total influenza vaccine failure of 1947 revisited: Major intrasubtypic antigenic change can explain failure of vaccine in a post-World War II epidemic
journal, July 2002

One-step immobilization of poly(dT)-modified DNA onto non-modified plastic substrates by UV irradiation for microarrays
journal, August 2006

Technology platforms for pharmacogenomic diagnostic assays
journal, September 2004

Inclusion of polyvinylpyrrolidone in the polymerase chain reaction reverses the inhibitory effects of polyphenolic contamination of RNA
journal, February 1999

Development of a Novel, Rapid Integrated Cryptosporidium parvum Detection Assay
journal, July 2000

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989

A ligase-mediated gene detection technique
journal, August 1988

The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick
journal, February 1997

Direct detection of potato leafroll virus in potato tubers by immunocapture and the isothermal nucleic acid amplification method NASBA
journal, June 1997

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
journal, October 2005