Global to push GA events into
skip to main content

Title: Increasing cell culture population doublings for long-term growth of finite life span human cell cultures

Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
Inventors:
;
Issue Date:
OSTI Identifier:
1171090
Assignee:
The Regents of the University of California (Oakland, CA) CHO
Patent Number(s):
8,962,325
Application Number:
12/242,474
Contract Number:
AC02-05CH11231
Resource Relation:
Patent File Date: 2008 Sep 30
Research Org:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Molecular changes accompanying senescence and immortalization of cultured human mammary epithelial cells
journal, November 2002
  • Yaswen, Paul; Stampfer, Martha R.
  • The International Journal of Biochemistry & Cell Biology, Vol. 34, Issue 11, p. 1382-1394
  • DOI: 10.1016/S1357-2725(02)00047-X

Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells
journal, April 2001
  • Stampfer, M. R.; Garbe, J.; Levine, G.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 8, p. 4498-4503
  • DOI: 10.1073/pnas.071483998

Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival
journal, August 2001
  • Oh, H.; Taffet, G. E.; Youker, K. A.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10308-10313
  • DOI: 10.1073/pnas.191169098

Telomerase protects developing neurons against DNA damage-induced cell death
journal, November 2001

Long-term culture of adult murine epidermal keratinocytes
journal, December 2005

Oxytocin and Oxytocin Receptors in Cancer Cells and Proliferation
journal, April 2004

Similar records in DOepatents and OSTI.GOV collections: