Increasing cell culture population doublings for long-term growth of finite life span human cell cultures
Abstract
Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1171090
- Patent Number(s):
- 8962325
- Application Number:
- 12/242,474
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008 Sep 30
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Stampfer, Martha R, and Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States: N. p., 2015.
Web.
Stampfer, Martha R, & Garbe, James C. Increasing cell culture population doublings for long-term growth of finite life span human cell cultures. United States.
Stampfer, Martha R, and Garbe, James C. Tue .
"Increasing cell culture population doublings for long-term growth of finite life span human cell cultures". United States. https://www.osti.gov/servlets/purl/1171090.
@article{osti_1171090,
title = {Increasing cell culture population doublings for long-term growth of finite life span human cell cultures},
author = {Stampfer, Martha R and Garbe, James C},
abstractNote = {Cell culture media formulations for culturing human epithelial cells are herein described. Also described are methods of increasing population doublings in a cell culture of finite life span human epithelial cells and prolonging the life span of human cell cultures. Using the cell culture media disclosed alone and in combination with addition to the cell culture of a compound associated with anti-stress activity achieves extended growth of pre-stasis cells and increased population doublings and life span in human epithelial cell cultures.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}
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Works referenced in this record:
Telomerase modulates expression of growth-controlling genes and enhances cell proliferation
journal, April 2003
- Smith, Laura L.; Coller, Hilary A.; Roberts, James M.
- Nature Cell Biology, Vol. 5, Issue 5
Defined systems for epithelial cell culture and use thereof
patent, February 2004
- Judd, David; Battista, Paul
- US Patent Document 6,692,961
Oxytocin enhances myoepithelial cell differentiation and proliferation in the mouse mammary gland.
journal, August 1993
- Sapino, A.; Macrì, L.; Tonda, L.
- Endocrinology, Vol. 133, Issue 2
Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
journal, January 1999
- Jiang, Xu-Rong; Jimenez, Gretchen; Chang, Edwin
- Nature Genetics, Vol. 21, Issue 1
Long-term culture of adult murine epidermal keratinocytes
journal, December 2005
- Yano, S.; Okochi, H.
- British Journal of Dermatology, Vol. 153, Issue 6, p. 1101-1104
Absence of cancer–associated changes in human fibroblasts immortalized with telomerase
journal, January 1999
- Morales, Carmela P.; Holt, Shawn E.; Ouellette, Michel
- Nature Genetics, Vol. 21, Issue 1
Telomerase protects developing neurons against DNA damage-induced cell death
journal, November 2001
- Lu, Chengbiao; Fu, Weiming; Mattson, Mark P.
- Developmental Brain Research, Vol. 131, Issue 1-2, p. 167-171
Enhanced growth medium and method for culturing human mammary epithelial cells
patent, December 1983
- Stampfer, Martha R.; Smith, Helene S.; Hackett, Adeline J.
- US Patent Document 4,423,145
Continuous human cell lines and method of making same
patent, February 1989
- Stampfer, Martha R.
- US Patent Document 4,808,532
Oxytocin and Oxytocin Receptors in Cancer Cells and Proliferation
journal, April 2004
- Cassoni, P.; Sapino, A.; Marrocco, T.
- Journal of Neuroendocrinology, Vol. 16, Issue 4, p. 362-364
Telomerase beyond telomeres
journal, August 2002
- Blasco, María A.
- Nature Reviews Cancer, Vol. 2, Issue 8
Increased epidermal tumors and increased skin wound healing in transgenic mice overexpressing the catalytic subunit of telomerase, mTERT, in basal keratinocytes
journal, June 2001
- Gonzalez-Suarez, E.
- The EMBO Journal, Vol. 20, Issue 11
Telomerase reverse transcriptase promotes cardiac muscle cell proliferation, hypertrophy, and survival
journal, August 2001
- Oh, H.; Taffet, G. E.; Youker, K. A.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10308-10313
Increased p16 expression with first senescence arrest in human mammary epithelial cells and extended growth capacity with p16 inactivation
journal, July 1998
- Brenner, Andrew J.; Stampfer, Martha R.; Aldaz, C. Marcelo
- Oncogene, Vol. 17, Issue 2
Expression of the telomerase catalytic subunit, hTERT, induces resistance to transforming growth factor β growth inhibition in p16INK4A(−) human mammary epithelial cells
journal, April 2001
- Stampfer, M. R.; Garbe, J.; Levine, G.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 8, p. 4498-4503