Restriction/modification polypeptides, polynucleotides, and methods

Title: Restriction/modification polypeptides, polynucleotides, and methods

The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.

Inventors:: Westpheling, Janet; Chung, DaeHwan; Huddleston, Jennifer; Farkas, Joel A

Issue Date:: 2015-02-24

OSTI Identifier:: 1171089

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA) ORO

Patent Number(s):: 8,962,333

Application Number:: 13/439,069

Contract Number:: AC05-00OR22725

Resource Relation:: Patent File Date: 2012 Apr 04

Research Org:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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