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Title: Restriction/modification polypeptides, polynucleotides, and methods

The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
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Issue Date:
OSTI Identifier:
University of Georgia Research Foundation, Inc. (Athens, GA) ORO
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2012 Apr 04
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
Country of Publication:
United States

Other works cited in this record:

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Energy production with hyperthermophilic organisms
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Gene knockout mesophilic and thermophilic organisms, and methods of use thereof
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