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Title: Restriction/modification polypeptides, polynucleotides, and methods

Abstract

The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.

Inventors:
; ; ;
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171089
Patent Number(s):
8962333
Application Number:
13/439,069
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Apr 04
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, and Farkas, Joel A. Restriction/modification polypeptides, polynucleotides, and methods. United States: N. p., 2015. Web.
Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, & Farkas, Joel A. Restriction/modification polypeptides, polynucleotides, and methods. United States.
Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, and Farkas, Joel A. Tue . "Restriction/modification polypeptides, polynucleotides, and methods". United States. https://www.osti.gov/servlets/purl/1171089.
@article{osti_1171089,
title = {Restriction/modification polypeptides, polynucleotides, and methods},
author = {Westpheling, Janet and Chung, DaeHwan and Huddleston, Jennifer and Farkas, Joel A},
abstractNote = {The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}

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