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Title: Restriction/modification polypeptides, polynucleotides, and methods

The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
Inventors:
; ; ;
Issue Date:
OSTI Identifier:
1171089
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA) ORO
Patent Number(s):
8,962,333
Application Number:
13/439,069
Contract Number:
AC05-00OR22725
Resource Relation:
Patent File Date: 2012 Apr 04
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Thermophile gene transfer
patent, February 1999

Energy production with hyperthermophilic organisms
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Method for the combined production of butanol and hydrogen
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Microorganisms and methods for carbon-efficient biosynthesis of MEK and 2-butanol
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Gene knockout mesophilic and thermophilic organisms, and methods of use thereof
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