Restriction/modification polypeptides, polynucleotides, and methods
Abstract
The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1171089
- Patent Number(s):
- 8,962,333
- Application Number:
- 13/439,069
- Assignee:
- University of Georgia Research Foundation, Inc. (Athens, GA)
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Apr 04
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, and Farkas, Joel A. Restriction/modification polypeptides, polynucleotides, and methods. United States: N. p., 2015.
Web.
Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, & Farkas, Joel A. Restriction/modification polypeptides, polynucleotides, and methods. United States.
Westpheling, Janet, Chung, DaeHwan, Huddleston, Jennifer, and Farkas, Joel A. Tue .
"Restriction/modification polypeptides, polynucleotides, and methods". United States. https://www.osti.gov/servlets/purl/1171089.
@article{osti_1171089,
title = {Restriction/modification polypeptides, polynucleotides, and methods},
author = {Westpheling, Janet and Chung, DaeHwan and Huddleston, Jennifer and Farkas, Joel A},
abstractNote = {The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}
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