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Title: Engineered microbes and methods for microbial oil production

Abstract

Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.

Inventors:
; ;
Issue Date:
Research Org.:
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1169615
Patent Number(s):
8951776
Application Number:
13/656,086
Assignee:
Massachusetts Institute of Technology (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AR0000059
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Stephanopoulos, Gregory, Tai, Mitchell, and Chakraborty, Sagar. Engineered microbes and methods for microbial oil production. United States: N. p., 2015. Web.
Stephanopoulos, Gregory, Tai, Mitchell, & Chakraborty, Sagar. Engineered microbes and methods for microbial oil production. United States.
Stephanopoulos, Gregory, Tai, Mitchell, and Chakraborty, Sagar. Tue . "Engineered microbes and methods for microbial oil production". United States. https://www.osti.gov/servlets/purl/1169615.
@article{osti_1169615,
title = {Engineered microbes and methods for microbial oil production},
author = {Stephanopoulos, Gregory and Tai, Mitchell and Chakraborty, Sagar},
abstractNote = {Some aspects of this invention provide engineered microbes for oil production. Methods for microbe engineering and for use of engineered microbes are also provided herein. In some embodiments, microbes are provided that are engineered to modulate a combination of rate-controlling steps of lipid synthesis, for example, a combination of a step generating metabolites, acetyl-CoA, ATP or NADPH for lipid synthesis (a push step), and a step sequestering a product or an intermediate of a lipid synthesis pathway that mediates feedback inhibition of lipid synthesis (a pull step). Such push-and-pull engineered microbes exhibit greatly enhanced conversion yields and TAG synthesis and storage properties.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}

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