Expanding the eukaryotic genetic code

Chin, Jason W; Cropp, T. Ashton; Anderson, J. Christopher; Schultz, Peter G

Title: Expanding the eukaryotic genetic code

Abstract

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

Inventors:: Chin, Jason W; Cropp, T. Ashton; Anderson, J. Christopher; Schultz, Peter G

Issue Date:: 2015-02-03

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1169093

Patent Number(s):: 8945926

Application Number:: 13/329,202

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K38/1709 - {from mammals}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P43/00 - Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/67 - General methods for enhancing the expression
C12N15/81 - for yeasts
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y601/01 - Ligases forming aminoacyl-tRNA and related compounds
C12Y601/01001 - Tyrosine-tRNA ligase
C12Y601/0102 - Phenylalanine-tRNA ligase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/81 - Packaged device or kit
Y10S435/975 - Kit

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DOE Contract Number:: FG0300ER45812

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Chin, Jason W, Cropp, T. Ashton, Anderson, J. Christopher, and Schultz, Peter G. Expanding the eukaryotic genetic code.  United States: N. p., 2015. 
        Web.

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                    Chin, Jason W, Cropp, T. Ashton, Anderson, J. Christopher, & Schultz, Peter G. Expanding the eukaryotic genetic code.  United States.

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                    Chin, Jason W, Cropp, T. Ashton, Anderson, J. Christopher, and Schultz, Peter G. Tue .  
        "Expanding the eukaryotic genetic code".  United States.  https://www.osti.gov/servlets/purl/1169093.

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@article{osti_1169093,

  title        = {Expanding the eukaryotic genetic code},

  author       = {Chin, Jason W and Cropp, T. Ashton and Anderson, J. Christopher and Schultz, Peter G},

  abstractNote = {This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {2}

}

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