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Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.
Inventors:
Issue Date:
OSTI Identifier:
1167243
Assignee:
The Scripps Research Institute (La Jolla, CA) CHO
Patent Number(s):
8,932,576
Application Number:
12/695,101
Contract Number:
FG03-02ER15313
Resource Relation:
Patent File Date: 2010 Jan 27
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Translational regulations as specific traits of chloroplast gene expression
journal, September 2002

tRNA gene number and codon usage in the C. elegans genome are co-adapted for optimal translation of highly expressed genes
journal, July 2000

Evolution of organellar genomes
journal, December 1999

Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum
journal, December 2001
  • McDonald, Thomas L.; Larson, Marilynn A.; Mack, David R.
  • Veterinary Immunology and Immunopathology, Vol. 83, Issue 3-4, p. 203-211
  • DOI: 10.1016/S0165-2427(01)00380-4

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