Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Mayfield, Stephen P

Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:: Mayfield, Stephen P

Issue Date:: 2015-01-13

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1167243

Patent Number(s):: 8932576

Application Number:: 12/695,101

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8214 - {Plastid transformation}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}
C12N15/79 - Vectors or expression systems specially adapted for eukaryotic hosts

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DOE Contract Number:: FG03-02ER15313

Resource Type:: Patent

Resource Relation:: Patent File Date: 2010 Jan 27

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast.  United States: N. p., 2015. 
        Web.

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                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast.  United States.

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                    Mayfield, Stephen P. Tue .  
        "Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast".  United States.  https://www.osti.gov/servlets/purl/1167243.

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@article{osti_1167243,

  title        = {Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast},

  author       = {Mayfield, Stephen P},

  abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {1}

}

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