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Title: Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167243
Patent Number(s):
8932576
Application Number:
12/695,101
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
FG03-02ER15313
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Jan 27
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. United States: N. p., 2015. Web.
Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. United States.
Mayfield, Stephen P. Tue . "Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast". United States. https://www.osti.gov/servlets/purl/1167243.
@article{osti_1167243,
title = {Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast},
author = {Mayfield, Stephen P},
abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {1}
}

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Works referenced in this record:

Participation of nuclear genes in chloroplast gene expression
journal, June 2000


Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes
journal, October 2005


Transgenic Plastids in Basic Research and Plant Biotechnology
journal, September 2001


Kanamycin resistance as a selectable marker for plastid transformation in tobacco
journal, October 1993


Translational regulations as specific traits of chloroplast gene expression
journal, September 2002


Co-variation of tRNA Abundance and Codon Usage inEscherichia coliat Different Growth Rates
journal, August 1996


Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast
journal, June 2002


Consecutive Low-usage Leucine Codons Block Translation Only When Near the 5′ End of a Message inEscherichia coli
journal, February 1995


Evolution of organellar genomes
journal, December 1999


Genetic engineering of the chloroplast
journal, June 2000


Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast
journal, May 2007


The Chlamydomonas reinhardtii Plastid Chromosome : Islands of Genes in a Sea of Repeats
journal, October 2002


Development of a luciferase reporter gene, luxCt , for Chlamydomonas reinhardtii chloroplast
journal, December 2003


Controlled expression of plastid transgenes in plants based on a nuclear DNA-encoded and plastid-targeted T7 RNA polymerase.
journal, July 1994


Elevated extrahepatic expression and secretion of mammary-associated serum amyloid A 3 (M-SAA3) into colostrum
journal, December 2001


Chloroplast DNA codon use: Evidence for selection at the psb A locus based on tRNA availability
journal, September 1993


Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker
journal, July 1999


High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
journal, February 1993