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Title: Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby

Abstract

Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.

Inventors:
;
Issue Date:
Research Org.:
Univ. of California, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167214
Patent Number(s):
8932859
Application Number:
12/124,888
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG03-01ER63276
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 May 21
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Tsien, Roger Y, and Wang, Lei. Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby. United States: N. p., 2015. Web.
Tsien, Roger Y, & Wang, Lei. Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby. United States.
Tsien, Roger Y, and Wang, Lei. Tue . "Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby". United States. https://www.osti.gov/servlets/purl/1167214.
@article{osti_1167214,
title = {Methods for engineering polypeptide variants via somatic hypermutation and polypeptide made thereby},
author = {Tsien, Roger Y and Wang, Lei},
abstractNote = {Methods using somatic hypermutation (SHM) for producing polypeptide and nucleic acid variants, and nucleic acids encoding such polypeptide variants are disclosed. Such variants may have desired properties. Also disclosed are novel polypeptides, such as improved fluorescent proteins, produced by the novel methods, and nucleic acids, vectors, and host cells comprising such vectors.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {1}
}

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Works referenced in this record:

Tight control of gene expression in mammalian cells by tetracycline-responsive promoters.
journal, June 1992


CD40 Stimulation of Human Peripheral B Lymphocytes: Distinct Response from Naive and Memory Cells
journal, October 2003


Increased Transcription Levels Induce Higher Mutation Rates in a Hypermutating Cell Line
journal, April 2001


A monomeric red fluorescent protein
journal, June 2002


Somatic Hypermutation in the Heavy Chain Locus Correlates with Transcription
journal, July 1998


The roots of antibody diversity
journal, September 2002


Death by Deamination: A Novel Host Restriction System for HIV-1
journal, August 2003


RNA Editing Enzyme APOBEC1 and Some of Its Homologs Can Act as DNA Mutators
journal, November 2002


Fluorescent proteins from nonbioluminescent Anthozoa species
journal, October 1999


Somatic hypermutation of the AID transgene in B and non-B cells
journal, August 2002


Activation-induced cytidine deaminase turns on somatic hypermutation in hybridomas
journal, January 2002


Crystal Structure of the Aequorea victoria Green Fluorescent Protein
journal, September 1996


Hypermutation of multiple proto-oncogenes in B-cell diffuse large-cell lymphomas
journal, July 2001


AID mutates E. coli suggesting a DNA deamination mechanism for antibody diversification
journal, July 2002


Evolutionary and somatic selection of the antibody repertoire in the mouse
journal, November 1987


Ablation of XRCC2/3 transforms immunoglobulin V gene conversion into somatic hypermutation
journal, August 2001


GFP-like Proteins as Ubiquitous Metazoan Superfamily: Evolution of Functional Features and Structural Complexity
journal, May 2004


Somatic hypermutation of immunoglobulin and non–immunoglobulin genes
journal, January 2001

  • Storb, Ursula; Ming Shen, Hong; Michael, Nancy
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 356, Issue 1405
  • https://doi.org/10.1098/rstb.2000.0743

Genome-wide somatic hypermutation
journal, April 2004


Evolution of new nonantibody proteins via iterative somatic hypermutation
journal, November 2004


A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins
journal, December 2001