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Title: Label-free functional nucleic acid sensors for detecting target agents

Abstract

A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described. In one example, a method for designing label-free fluorescent functional nucleic acid sensors (e.g., those that include a DNAzyme, aptamer or aptazyme) that have a tunable dynamic range through the introduction of an abasic site (e.g., dSpacer) or a vacant site into the functional nucleic acids. Also provided is a general method for designing label-free fluorescent aptamer sensors based on the regulation of malachite green (MG) fluorescence. A general method for designing label-free fluorescent catalytic and molecular beacons (CAMBs) is also provided. The methods demonstrated here can be used to design many other label-free fluorescent sensors to detect a wide range of analytes. Sensors and methods of using the disclosed sensors are also provided.

Inventors:
;
Issue Date:
Research Org.:
Univ. of Illinois at Urbana-Champaign, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167209
Patent Number(s):
8933210
Application Number:
13/267,414
Assignee:
The Board of Trustees of the University of Illinois (Urbana, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
DOE Contract Number:  
FG02-08ER64568
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Oct 06
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lu, Yi, and Xiang, Yu. Label-free functional nucleic acid sensors for detecting target agents. United States: N. p., 2015. Web.
Lu, Yi, & Xiang, Yu. Label-free functional nucleic acid sensors for detecting target agents. United States.
Lu, Yi, and Xiang, Yu. Tue . "Label-free functional nucleic acid sensors for detecting target agents". United States. https://www.osti.gov/servlets/purl/1167209.
@article{osti_1167209,
title = {Label-free functional nucleic acid sensors for detecting target agents},
author = {Lu, Yi and Xiang, Yu},
abstractNote = {A general methodology to design label-free fluorescent functional nucleic acid sensors using a vacant site approach and an abasic site approach is described. In one example, a method for designing label-free fluorescent functional nucleic acid sensors (e.g., those that include a DNAzyme, aptamer or aptazyme) that have a tunable dynamic range through the introduction of an abasic site (e.g., dSpacer) or a vacant site into the functional nucleic acids. Also provided is a general method for designing label-free fluorescent aptamer sensors based on the regulation of malachite green (MG) fluorescence. A general method for designing label-free fluorescent catalytic and molecular beacons (CAMBs) is also provided. The methods demonstrated here can be used to design many other label-free fluorescent sensors to detect a wide range of analytes. Sensors and methods of using the disclosed sensors are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {1}
}

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Works referenced in this record:

Label-Free Fluorescent Functional DNA Sensors Using Unmodified DNA: A Vacant Site Approach
journal, May 2010


A Lead-Dependent DNAzyme with a Two-Step Mechanism
journal, June 2003


Biochemical Characterization of a Uranyl Ion-Specific DNAzyme
journal, February 2009


Fast Colorimetric Sensing of Adenosine and Cocaine Based on a General Sensor Design Involving Aptamers and Nanoparticles
journal, January 2006


Structure-Switching Signaling Aptamers
journal, April 2003


A DNA-Protein Nanoengine for “On-Demand” Release and Precise Delivery of Molecules
journal, August 2005


Label-Free Fluorescent Aptamer Sensor Based on Regulation of Malachite Green Fluorescence
journal, January 2010