skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Pyrococcus furiosus strains and methods of using same

Abstract

Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.

Inventors:
; ; ;
Issue Date:
Research Org.:
University of Georgia Research Foundation, Inc., Athens, GA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1167026
Patent Number(s):
8,927,254
Application Number:
13/248,377
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
DOE Contract Number:  
FG05-95ER20175
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Sep 29
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., and Westpheling, Janet. Pyrococcus furiosus strains and methods of using same. United States: N. p., 2015. Web.
Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., & Westpheling, Janet. Pyrococcus furiosus strains and methods of using same. United States.
Lipscomb, Gina L, Farkas, Joel Andrew, Adams, Michael W. W., and Westpheling, Janet. Tue . "Pyrococcus furiosus strains and methods of using same". United States. https://www.osti.gov/servlets/purl/1167026.
@article{osti_1167026,
title = {Pyrococcus furiosus strains and methods of using same},
author = {Lipscomb, Gina L and Farkas, Joel Andrew and Adams, Michael W. W. and Westpheling, Janet},
abstractNote = {Provided herein are methods for transforming a Pyrococcus furiosus with a polynucleotide. In one embodiment, the method includes contacting a P. furiosus with a polynucleotide under conditions suitable for uptake of the polynucleotide by the P. furiosus, and identifying transformants at a frequency of, for instance, at least 10.sup.3 transformants per microgram DNA. Also provided are isolated Pyrococcus furiosus having the characteristics of Pyrococcus furiosus COM1, and plasmids that include an origin of replication that functions in a Pyrococcus furiosus. The plasmid is stable in a recipient P. furiosus without selection for more than 100 generations and is structurally unchanged after replication in P. furiosus for more than 100 generations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Key Role for Sulfur in Peptide Metabolism and in Regulation of Three Hydrogenases in the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, January 2001


Development of Additional Selectable Markers for the Halophilic Archaeon Haloferax volcanii Based on the leuB and trpA Genes
journal, February 2004


Archaeal genetics — the third way
journal, January 2005

  • Allers, Thorsten; Mevarech, Moshe
  • Nature Reviews Genetics, Vol. 6, Issue 1
  • DOI: 10.1038/nrg1504

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

  • Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A.
  • Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402
  • DOI: 10.1093/nar/25.17.3389

Recent progress towards the application of hyperthermophiles and their enzymes
journal, April 2005


Shuffling genes around in hot environments: the unique DNA transporter of Thermus thermophilus
journal, May 2009


Genetic tools for Sulfolobus spp.: vectors and first applications
journal, June 2008


An Archaeal Chromosomal Autonomously Replicating Sequence Element from an Extreme Halophile, Halobacterium sp. Strain NRC-1
journal, October 2003


A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
journal, November 1984

  • Boeke, Jef D.; La Croute, Francois; Fink, Gerald R.
  • Molecular and General Genetics MGG, Vol. 197, Issue 2
  • DOI: 10.1007/BF00330984

The genetic transformation machinery: composition, localization, and mechanism
journal, May 2009


Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus
journal, August 2004


Unmarked gene deletion and host–vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus
journal, June 2009


Interdomain Conjugal Transfer of DNA from Bacteria to Archaea
journal, June 2010

  • Dodsworth, J. A.; Li, L.; Wei, S.
  • Applied and Environmental Microbiology, Vol. 76, Issue 16
  • DOI: 10.1128/AEM.00967-10

Industrial relevance of thermophilic Archaea
journal, December 2005

  • Egorova, Ksenia; Antranikian, Garabed
  • Current Opinion in Microbiology, Vol. 8, Issue 6, p. 649-655
  • DOI: 10.1016/j.mib.2005.10.015

Pyrococcus abyssi sp. nov., a new hyperthermophilic archaeon isolated from a deep-sea hydrothermal vent
journal, November 1993

  • Erauso, Ga�l; Reysenbach, Anna-Louise; Godfroy, Anne
  • Archives of Microbiology, Vol. 160, Issue 5
  • DOI: 10.1007/BF00252219

Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C
journal, June 1986

  • Fiala, Gerhard; Stetter, Karl O.
  • Archives of Microbiology, Vol. 145, Issue 1, p. 56-61
  • DOI: 10.1007/BF00413027

Homologous recombination in Sulfolobus acidocaldarius: genetic assays and functional properties
journal, January 2009

  • Grogan, Dennis W.
  • Biochemical Society Transactions, Vol. 37, Issue 1
  • DOI: 10.1042/BST0370088

Host—vector systems in thermophilic Bacilli and their applications
journal, November 1983


Hydrogenases of the Model Hyperthermophiles
journal, March 2008

  • Jenney, Francis E.; Adams, Michael W. W.
  • Annals of the New York Academy of Sciences, Vol. 1125, Issue 1
  • DOI: 10.1196/annals.1419.013

Natural genetic transformation: prevalence, mechanisms and function
journal, December 2007

  • Johnsborg, Ola; Eldholm, Vegard; Håvarstein, Leiv Sigve
  • Research in Microbiology, Vol. 158, Issue 10, p. 767-778
  • DOI: 10.1016/j.resmic.2007.09.004

Homologous recombination of exogenous DNA with the Sulfolobus acidocaldarius genome: Properties and uses
journal, December 2005


Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases
journal, February 2011

  • Lipscomb, Gina L.; Stirrett, Karen; Schut, Gerrit J.
  • Applied and Environmental Microbiology, Vol. 77, Issue 7, p. 2232-2238
  • DOI: 10.1128/AEM.02624-10

Construction of a Shuttle Vector for, and Spheroplast Transformation of, the Hyperthermophilic Archaeon Pyrococcus abyssi
journal, November 2002


Characterization of Hydrogenase II from the Hyperthermophilic Archaeon Pyrococcus furiosus and Assessment of Its Role in Sulfur Reduction
journal, April 2000


Disruption of a Sugar Transporter Gene Cluster in a Hyperthermophilic Archaeon Using a Host-Marker System Based on Antibiotic Resistance
journal, January 2007

  • Matsumi, R.; Manabe, K.; Fukui, T.
  • Journal of Bacteriology, Vol. 189, Issue 7, p. 2683-2691
  • DOI: 10.1128/JB.01692-06

In vivo interactions of archaeal Cdc6/Orc1 and minichromosome maintenance proteins with the replication origin
journal, September 2001

  • Matsunaga, F.; Forterre, P.; Ishino, Y.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 20, p. 11152-11157
  • DOI: 10.1073/pnas.191387498

Genomewide and biochemical analyses of DNA-binding activity of Cdc6/Orc1 and Mcm proteins in Pyrococcus sp.
journal, April 2007

  • Matsunaga, Fujihiko; Glatigny, Annie; Mucchielli-Giorgi, Marie-Hélène
  • Nucleic Acids Research, Vol. 35, Issue 10
  • DOI: 10.1093/nar/gkm212

Recent advances in genetic analyses of hyperthermophilic Archaea and Bacteria
journal, August 1997


Replication origin of the Escherichia coli K-12 chromosome: The size and structure of the minimum DNA segment carrying the information for autonomous replication
journal, April 1980

  • Oka, Atsuhiro; Sugimoto, Kazunori; Takanami, Mituru
  • MGG Molecular & General Genetics, Vol. 178, Issue 1
  • DOI: 10.1007/BF00267207

The extremely halophilic archaeon Haloferax volcanii has two very different dihydrofolate reductases: Haloferax volcanii dihydrofolate reductases
journal, March 2000


Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker: ura3-based gene knockout in H. salinarum
journal, February 2000


Identification of Two Origins of Replication in the Single Chromosome of the Archaeon Sulfolobus solfataricus
journal, January 2004


Genetic technologies for Archaea
journal, December 2005

  • Rother, Michael; Metcalf, William W.
  • Current Opinion in Microbiology, Vol. 8, Issue 6, p. 745-751
  • DOI: 10.1016/j.mib.2005.10.010

Polarity in Archaeal Operon Transcription in Thermococcus kodakaraensis
journal, January 2008

  • Santangelo, T. J.; Cubonova, L.; Matsumi, R.
  • Journal of Bacteriology, Vol. 190, Issue 6, p. 2244-2248
  • DOI: 10.1128/JB.01811-07

Shuttle Vector Expression in Thermococcus kodakaraensis: Contributions of cis Elements to Protein Synthesis in a Hyperthermophilic Archaeon
journal, March 2008

  • Santangelo, T. J.; Cubonova, L.; Reeve, J. N.
  • Applied and Environmental Microbiology, Vol. 74, Issue 10
  • DOI: 10.1128/AEM.00305-08

Thermococcus kodakarensis Genetics: TK1827-Encoded  -Glycosidase, New Positive-Selection Protocol, and Targeted and Repetitive Deletion Technology
journal, December 2009

  • Santangelo, T. J.; Cubonova, L.; Reeve, J. N.
  • Applied and Environmental Microbiology, Vol. 76, Issue 4
  • DOI: 10.1128/AEM.02497-09

Targeted Gene Disruption by Homologous Recombination in the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
journal, January 2003


Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A- Dependent NAD(P)H Sulfur Oxidoreductase
journal, April 2007

  • Schut, G. J.; Bridger, S. L.; Adams, M. W. W.
  • Journal of Bacteriology, Vol. 189, Issue 12, p. 4431-4441
  • DOI: 10.1128/JB.00031-07

DNA Microarray Analysis of the Hyperthermophilic Archaeon Pyrococcus furiosus: Evidence for a New Type of Sulfur-Reducing Enzyme Complex
journal, December 2001


From genomes to function: haloarchaea as model organisms
journal, March 2006


A polymerase III-like reinitiation mechanism is operating in regulation of histone expression in archaea
journal, March 2008


Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production
journal, May 2010


A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance
journal, April 2000


Genetics of Methanococcus: possibilities for functional genomics in Archaea
journal, July 1999


Hydrogen production by hyperthermophilic and extremely thermophilic bacteria and archaea: mechanisms for reductant disposal
journal, July 2010

  • Verhaart, Marcel R. A.; Bielen, Abraham A. M.; Oost, John van der
  • Environmental Technology, Vol. 31, Issue 8-9
  • DOI: 10.1080/09593331003710244

Shuttle Vector-Based Transformation System for Pyrococcus furiosus
journal, April 2010

  • Waege, I.; Schmid, G.; Thumann, S.
  • Applied and Environmental Microbiology, Vol. 76, Issue 10
  • DOI: 10.1128/AEM.01951-09

Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus
journal, January 2009

  • Wagner, Michaela; Berkner, Silvia; Ajon, Malgorzata
  • Biochemical Society Transactions, Vol. 37, Issue 1
  • DOI: 10.1042/BST0370097

Targeted Disruption of the  -Amylase Gene in the Hyperthermophilic Archaeon Sulfolobus solfataricus
journal, January 2003


Genetic modification of homolactic thermophilic bacilli
patent, July 2013