Pnp gene modification for improved xylose utilization in Zymomonas
Abstract
The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.
- Inventors:
- Issue Date:
- Research Org.:
- E I Du Pont de Nemours and Company, Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1165440
- Patent Number(s):
- 8911983
- Application Number:
- 13/711,646
- Assignee:
- E I Du Pont de Nemours and Company (Wilmington, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC36-07GO17056
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Dec 12
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States: N. p., 2014.
Web.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, & Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Tue .
"Pnp gene modification for improved xylose utilization in Zymomonas". United States. https://www.osti.gov/servlets/purl/1165440.
@article{osti_1165440,
title = {Pnp gene modification for improved xylose utilization in Zymomonas},
author = {Caimi, Perry G G and Qi, Min and Tao, Luan and Viitanen, Paul V and Yang, Jianjun},
abstractNote = {The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}
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Works referencing / citing this record:
Enhancing D-xylose and L-arabinose utilization in Zymomonas cells
patent, June 2016
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