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Title: Pnp gene modification for improved xylose utilization in Zymomonas

Abstract

The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
E I Du Pont de Nemours and Company, Wilmington, DE (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1165440
Patent Number(s):
8911983
Application Number:
13/711,646
Assignee:
E I Du Pont de Nemours and Company (Wilmington, DE)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC36-07GO17056
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Dec 12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS

Citation Formats

Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States: N. p., 2014. Web.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, & Yang, Jianjun. Pnp gene modification for improved xylose utilization in Zymomonas. United States.
Caimi, Perry G G, Qi, Min, Tao, Luan, Viitanen, Paul V, and Yang, Jianjun. Tue . "Pnp gene modification for improved xylose utilization in Zymomonas". United States. https://www.osti.gov/servlets/purl/1165440.
@article{osti_1165440,
title = {Pnp gene modification for improved xylose utilization in Zymomonas},
author = {Caimi, Perry G G and Qi, Min and Tao, Luan and Viitanen, Paul V and Yang, Jianjun},
abstractNote = {The endogenous pnp gene encoding polynucleotide phosphorylase in the Zymomonas genome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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Works referenced in this record:

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journal, January 2005


Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
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Function of the Conserved S1 and KH Domains in Polynucleotide Phosphorylase
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Polynucleotide Phosphorylase Activity May Be Modulated by Metabolites in Escherichia coli
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Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
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Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose
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Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
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Amplification of ribonuclease II( mb ) activity in Escherichia coli K-12
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Global analysis of Escherichia coli RNA degradosome function using DNA microarrays
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