skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Abstract

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.

Inventors:
; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1165126
Patent Number(s):
8907061
Application Number:
12/352,548
Assignee:
Lawrence Livermore National Security, LLC. (Livermore, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Resource Relation:
Patent File Date: 2009 Jan 12
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chromy, Brett A, Henderson, Paul, and Hoeprich, Jr., Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States: N. p., 2014. Web.
Chromy, Brett A, Henderson, Paul, & Hoeprich, Jr., Paul D. Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification. United States.
Chromy, Brett A, Henderson, Paul, and Hoeprich, Jr., Paul D. Tue . "Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification". United States. https://www.osti.gov/servlets/purl/1165126.
@article{osti_1165126,
title = {Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification},
author = {Chromy, Brett A and Henderson, Paul and Hoeprich, Jr., Paul D},
abstractNote = {Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

Patent:

Save / Share:

Works referenced in this record:

Atomic force microscopy differentiates discrete size distributions between membrane protein containing and empty nanolipoprotein particles
journal, March 2009


Functional Cell-free Synthesis of a Seven Helix Membrane Protein: In situ Insertion of Bacteriorhodopsin into Liposomes
journal, August 2007


Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
journal, June 2006


Reconstitution and Imaging of a Membrane Protein in a Nanometer-Size Phospholipid Bilayer
journal, September 1998


Carboxyl-Terminal Domain of Human Apolipoprotein E: Expression, Purification, and Crystallization
journal, November 1999


Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
journal, July 1999


Structural Basis for Thermal Stability of Human Low-Density Lipoprotein
journal, March 2005


Complex of Human Apolipoprotein C-1 with Phospholipid:  Thermodynamic or Kinetic Stability?
journal, June 2002


Asp 46 can substitute for Asp 96 as the Schiff base proton donor in bacteriorhodopsin
journal, November 1995


High level cell-free expression and specific labeling of integral membrane proteins
journal, February 2004


Cell-free expression as an emerging technique for the large scale production of integral membrane protein
journal, September 2006


Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004

  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731
  • https://doi.org/10.1021/ja0456611

Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins
journal, August 2004


Laboratory scale structural genomics
journal, March 2004


Conformational Reorganization of the Four-helix Bundle of Human Apolipoprotein E in Binding to Phospholipid
journal, May 2000


Electron microscopic study on reassembly of plasma high density apoprotein with various lipids
journal, November 1971


Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003


Do Recombinant Receptor Assays Provide Affinity and Provide Estimates?
journal, May 1997


Peptide stabilized amphotericin B nanodisks
journal, April 2007


Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins
journal, August 2002


Comparison of the Dynamics of the Primary Events of Bacteriorhodopsin in Its Trimeric and Monomeric States
journal, September 2002


Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, June 2000


A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003


In-depth analysis of the membrane and cytosolic proteome of red blood cells
journal, August 2006


Charge‐reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses
journal, September 2001

  • Bacher, Gerold; Szymanski, Wladyslaw W.; Kaufman, Stanley L.
  • Journal of Mass Spectrometry, Vol. 36, Issue 9, p. 1038-1052
  • https://doi.org/10.1002/jms.208

Hydrogen Production under Aerobic Conditions by Membrane-Bound Hydrogenases from Ralstonia Species
journal, August 2008


A novel approach for biohydrogen production
journal, September 2006


Fabrication of biomolecule–copolymer hybrid nanovesicles as energy conversion systems
journal, November 2005


From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels
journal, October 2005


High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007


Chemistry: The photon trap
journal, March 2008


In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996


Enzymatic production of biohydrogen: Biotechnology
journal, June 2000


Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels
journal, October 2005


Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor
journal, August 2008


Quantifying size distributions of nanolipoprotein particles with single-particle analysis and molecular dynamic simulations
journal, April 2008


Different Apolipoproteins Impact Nanolipoprotein Particle Formation
journal, November 2007

  • Chromy, Brett A.; Arroyo, Erin; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 129, Issue 46, p. 14348-14354
  • https://doi.org/10.1021/ja074753y

Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins
journal, February 2007


Energy-Converting [NiFe] Hydrogenases from Archaea and Extremophiles: Ancestors of Complex I
journal, February 2004


Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007


Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008


Membrane-Mimetic Films of Asymmetric Phosphatidylcholine Lipid Bolaamphiphiles
journal, January 2006


[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007


Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
journal, October 2007


The Difference a Se Makes? Oxygen-Tolerant Hydrogen Production by the [NiFeSe]-Hydrogenase from Desulfomicrobium baculatum
journal, October 2008


High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008


Topology prediction of membrane proteins
journal, February 1996