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Title: Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification

Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
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Issue Date:
OSTI Identifier:
Lawrence Livermore National Security, LLC. (Livermore, CA) LSO
Patent Number(s):
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Resource Relation:
Patent File Date: 2009 Jan 12
Research Org:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

Atomic force microscopy differentiates discrete size distributions between membrane protein containing and empty nanolipoprotein particles
journal, March 2009
  • Blanchette, Craig D.; Cappuccio, Jenny A.; Kuhn, Edward A.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1788, Issue 3, p. 724-731
  • DOI: 10.1016/j.bbamem.2008.11.019

Functional Cell-free Synthesis of a Seven Helix Membrane Protein: In situ Insertion of Bacteriorhodopsin into Liposomes
journal, August 2007
  • Kalmbach, Rolf; Chizhov, Igor; Schumacher, Miria C.
  • Journal of Molecular Biology, Vol. 371, Issue 3, p. 639-648
  • DOI: 10.1016/j.jmb.2007.05.087

Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
journal, June 2006
  • Bayburt, Timothy H.; Grinkova, Yelena V.; Sligar, Stephen G.
  • Archives of Biochemistry and Biophysics, Vol. 450, Issue 2, p. 215-222
  • DOI: 10.1016/

Structural Basis for Thermal Stability of Human Low-Density Lipoprotein
journal, March 2005
  • Jayaraman, Shobini; Gantz, Donald; Gursky, Olga
  • Biochemistry, Vol. 44, Issue 10, p. 3965-3971
  • DOI: 10.1021/bi047493v

High level cell-free expression and specific labeling of integral membrane proteins
journal, February 2004

Cell-free expression as an emerging technique for the large scale production of integral membrane protein
journal, September 2006

Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004
  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731
  • DOI: 10.1021/ja0456611

Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins
journal, August 2004
  • Rao, Rupa S.; Visuri, Steven R.; McBride, Mary T.
  • Journal of Proteome Research, Vol. 3, Issue 4, p. 736-742
  • DOI: 10.1021/pr034130t

Laboratory scale structural genomics
journal, March 2004
  • Segelke, Brent W.; Schafer, Johana; Coleman, Matthew A.
  • Journal of Structural and Functional Genomics, Vol. 5, Issue 1-2, p. 147-157
  • DOI: 10.1023/B:JSFG.0000029193.82120.d1

Conformational Reorganization of the Four-helix Bundle of Human Apolipoprotein E in Binding to Phospholipid
journal, May 2000
  • Lu, Bin; Morrow, Julie A.; Weisgraber, Karl H.
  • Journal of Biological Chemistry, Vol. 275, Issue 27, p. 20775-20781
  • DOI: 10.1074/jbc.M003508200

Electron microscopic study on reassembly of plasma high density apoprotein with various lipids
journal, November 1971
  • Forte, T. M.; Nichols, A. V.; Gong, E. L.
  • Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 248, Issue 2, p. 381-386
  • DOI: 10.1016/0005-2760(71)90026-9

Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003
  • Bayburt, Timothy H.; Sligar, Stephen G.
  • Protein Science, Vol. 12, Issue 11, p. 2476-2481
  • DOI: 10.1110/ps.03267503

Peptide stabilized amphotericin B nanodisks
journal, April 2007

Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins
journal, August 2002
  • Bayburt, Timothy H.; Grinkova, Yelena V.; Sligar, Stephen G.
  • Nano Letters, Vol. 2, Issue 8, p. 853-856
  • DOI: 10.1021/nl025623k

Comparison of the Dynamics of the Primary Events of Bacteriorhodopsin in Its Trimeric and Monomeric States
journal, September 2002

Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, June 2000

A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003
  • Sapra, R.; Bagramyan, K.; Adams, M. W. W.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 13, p. 7545-7550
  • DOI: 10.1073/pnas.1331436100

Charge‐reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses
journal, September 2001
  • Bacher, Gerold; Szymanski, Wladyslaw W.; Kaufman, Stanley L.
  • Journal of Mass Spectrometry, Vol. 36, Issue 9, p. 1038-1052
  • DOI: 10.1002/jms.208

A novel approach for biohydrogen production
journal, September 2006

From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels
journal, October 2005
  • Vincent, K. A.; Cracknell, J. A.; Lenz, O.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 47, p. 16951-16954
  • DOI: 10.1073/pnas.0504499102

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007
  • Zhang, Y.-H. Percival; Evans, Barbara R.; Mielenz, Jonathan R.
  • PLoS ONE, Vol. 2, Issue 5, Article No. e456
  • DOI: 10.1371/journal.pone.0000456

In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996
  • Woodward, Jonathan; Mattingly, Susan M.; Danson, Michael
  • Nature Biotechnology, Vol. 14, Issue 7, p. 872-874
  • DOI: 10.1038/nbt0796-872

Enzymatic production of biohydrogen: Biotechnology
journal, June 2000
  • Woodward, Jonathan; Orr, Mark; Cordray, Kimberley
  • Nature, Vol. 405, Issue 6790, p. 1014-1015
  • DOI: 10.1038/35016633

Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels
journal, October 2005
  • Elgren, Timothy E.; Zadvorny, Oleg A.; Brecht, Eric
  • Nano Letters, Vol. 5, Issue 10, p. 2085-2087
  • DOI: 10.1021/nl0512224

Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor
journal, August 2008
  • Borch, Jonas; Torta, Federico; Sligar, Stephen G.
  • Analytical Chemistry, Vol. 80, Issue 16, p. 6245-6252
  • DOI: 10.1021/ac8000644

Different Apolipoproteins Impact Nanolipoprotein Particle Formation
journal, November 2007
  • Chromy, Brett A.; Arroyo, Erin; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 129, Issue 46, p. 14348-14354
  • DOI: 10.1021/ja074753y

Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007
  • Vignais, Paulette M.; Billoud, Bernard
  • Chemical Reviews, Vol. 107, Issue 10, p. 4206-4272
  • DOI: 10.1021/cr050196r

[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007

Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
journal, October 2007
  • Vincent, Kylie A.; Parkin, Alison; Armstrong, Fraser A.
  • Chemical Reviews, Vol. 107, Issue 10, p. 4366-4413
  • DOI: 10.1021/cr050191u

High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008
  • Wuu, Jessica J.; Swartz, James R.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1778, Issue 5, p. 1237-1250
  • DOI: 10.1016/j.bbamem.2008.01.023