Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid
Abstract
Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1164688
- Patent Number(s):
- 8900835
- Application Number:
- 13/301,836
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FG36-04GO14019
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2011 Nov 22
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 54 ENVIRONMENTAL SCIENCES
Citation Formats
Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States: N. p., 2014.
Web.
Wang, Qingzhao, Shanmugam, Keelnatham T, & Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States.
Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Tue .
"Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid". United States. https://www.osti.gov/servlets/purl/1164688.
@article{osti_1164688,
title = {Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid},
author = {Wang, Qingzhao and Shanmugam, Keelnatham T and Ingram, Lonnie O},
abstractNote = {Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 02 00:00:00 EST 2014},
month = {Tue Dec 02 00:00:00 EST 2014}
}
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