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Title: Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid

Abstract

Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.

Inventors:
; ;
Issue Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1164688
Patent Number(s):
8900835
Application Number:
13/301,836
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG36-04GO14019
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Nov 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 54 ENVIRONMENTAL SCIENCES

Citation Formats

Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States: N. p., 2014. Web.
Wang, Qingzhao, Shanmugam, Keelnatham T, & Ingram, Lonnie O. Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid. United States.
Wang, Qingzhao, Shanmugam, Keelnatham T, and Ingram, Lonnie O. Tue . "Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid". United States. https://www.osti.gov/servlets/purl/1164688.
@article{osti_1164688,
title = {Engineering of thermotolerant Bacillus coagulans for production of D(-)-lactic acid},
author = {Wang, Qingzhao and Shanmugam, Keelnatham T and Ingram, Lonnie O},
abstractNote = {Genetically modified microorganisms having the ability to produce D(-)-lactic acid at temperatures between 30.degree. C. and 55.degree. C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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Works referenced in this record:

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