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Title: Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Abstract

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

Inventors:
; ;
Issue Date:
Research Org.:
George Washington Univ., Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1164685
Patent Number(s):
8901487
Application Number:
13/045,277
Assignee:
George Washington University (Washington, DC)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
DOE Contract Number:  
FG02-01ER15129
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Mar 10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION

Citation Formats

Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry. United States: N. p., 2014. Web.
Vertes, Akos, Stolee, Jessica A, & Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry. United States.
Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Tue . "Subcellular analysis by laser ablation electrospray ionization mass spectrometry". United States. https://www.osti.gov/servlets/purl/1164685.
@article{osti_1164685,
title = {Subcellular analysis by laser ablation electrospray ionization mass spectrometry},
author = {Vertes, Akos and Stolee, Jessica A and Shrestha, Bindesh},
abstractNote = {In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

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