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Title: Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Abstract

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

Inventors:
; ;
Issue Date:
Research Org.:
George Washington Univ., Washington, DC (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1164685
Patent Number(s):
8901487
Application Number:
13/045,277
Assignee:
George Washington University (Washington, DC)
Patent Classifications (CPCs):
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
H - ELECTRICITY H05 - ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR H05H - PLASMA TECHNIQUE
DOE Contract Number:  
FG02-01ER15129
Resource Type:
Patent
Resource Relation:
Patent File Date: 2011 Mar 10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION

Citation Formats

Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry. United States: N. p., 2014. Web.
Vertes, Akos, Stolee, Jessica A, & Shrestha, Bindesh. Subcellular analysis by laser ablation electrospray ionization mass spectrometry. United States.
Vertes, Akos, Stolee, Jessica A, and Shrestha, Bindesh. Tue . "Subcellular analysis by laser ablation electrospray ionization mass spectrometry". United States. https://www.osti.gov/servlets/purl/1164685.
@article{osti_1164685,
title = {Subcellular analysis by laser ablation electrospray ionization mass spectrometry},
author = {Vertes, Akos and Stolee, Jessica A and Shrestha, Bindesh},
abstractNote = {In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {12}
}

Works referenced in this record:

Free-electron-laser-based biophysical and biomedical instrumentation
journal, July 2003


FT-IR imaging of native and tissue-engineered bone and cartilage
journal, May 2007


Matrix-assisted laser desorption and ionization in the OH and CO absorption bands of aliphatic and aromatic matrices: dependence on laser wavelength and temporal beam profile
journal, December 1997


Ablation and analysis of small cell populations and single cells by consecutive laser pulses
journal, June 2010


Nanoscale Atmospheric Pressure Laser Ablation-Mass Spectrometry
journal, April 2001


New dimension in nano-imaging: breaking through the diffraction limit with scanning near-field optical microscopy
journal, November 2004


Direct Protein Detection from Biological Media through Electrospray-Assisted Laser Desorption Ionization/Mass Spectrometry
journal, May 2006


Versatile New Ion Source for the Analysis of Materials in Open Air under Ambient Conditions
journal, April 2005


Laser Ablation Electrospray Ionization for Atmospheric Pressure, in Vivo, and Imaging Mass Spectrometry
journal, November 2007


Recent Advances in Optical Tweezers
journal, June 2008


In Situ Metabolic Profiling of Single Cells by Laser Ablation Electrospray Ionization Mass Spectrometry
journal, October 2009


Intact and top-down characterization of biomolecules and direct analysis using infrared matrix-assisted laser desorption electrospray ionization coupled to FT-ICR mass spectrometry
journal, April 2009


Infrared laser-assisted desorption electrospray ionization mass spectrometry
journal, January 2008


Infrared Laser Ablation Atmospheric Pressure Photoionization Mass Spectrometry
journal, January 2012


Laser Capture Microdissection
journal, November 1996