Title: Subcellular analysis by laser ablation electrospray ionization mass spectrometry

Abstract

In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

Inventors:

Vertes, Akos; Stolee, Jessica A; Shrestha, Bindesh

Issue Date:

2014-12-02

Research Org.:

George Washington Univ., Washington, DC (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1164685

Patent Number(s):

8901487

Application Number:

13/045,277

Assignee:

George Washington University (Washington, DC)

Patent Classifications (CPCs):

H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS

H - ELECTRICITY H05 - ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR H05H - PLASMA TECHNIQUE

Show more

H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS

H01J49/26 - Mass spectrometers or separator tubes

H - ELECTRICITY H05 - ELECTRIC TECHNIQUES NOT OTHERWISE PROVIDED FOR H05H - PLASMA TECHNIQUE

H05H3/02 - Molecular or atomic beam generation {

Show less

DOE Contract Number:  

FG02-01ER15129

Resource Type:

Patent

Resource Relation:

Patent File Date: 2011 Mar 10

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 47 OTHER INSTRUMENTATION