skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Semiconductor nanocrystal-based phagokinetic tracking

Abstract

Methods for determining metabolic properties of living cells through the uptake of semiconductor nanocrystals by cells. Generally the methods require a layer of neutral or hydrophilic semiconductor nanocrystals and a layer of cells seeded onto a culture surface and changes in the layer of semiconductor nanocrystals are detected. The observed changes made to the layer of semiconductor nanocrystals can be correlated to such metabolic properties as metastatic potential, cell motility or migration.

Inventors:
; ; ; ;
Issue Date:
Research Org.:
Oakland Operations Office, Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1163998
Patent Number(s):
8,889,414
Application Number:
10/285,187
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC03-76SF00098
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 36 MATERIALS SCIENCE; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats

Alivisatos, A Paul, Larabell, Carolyn A, Parak, Wolfgang J, Le Gros, Mark, and Boudreau, Rosanne. Semiconductor nanocrystal-based phagokinetic tracking. United States: N. p., 2014. Web.
Alivisatos, A Paul, Larabell, Carolyn A, Parak, Wolfgang J, Le Gros, Mark, & Boudreau, Rosanne. Semiconductor nanocrystal-based phagokinetic tracking. United States.
Alivisatos, A Paul, Larabell, Carolyn A, Parak, Wolfgang J, Le Gros, Mark, and Boudreau, Rosanne. Tue . "Semiconductor nanocrystal-based phagokinetic tracking". United States. https://www.osti.gov/servlets/purl/1163998.
@article{osti_1163998,
title = {Semiconductor nanocrystal-based phagokinetic tracking},
author = {Alivisatos, A Paul and Larabell, Carolyn A and Parak, Wolfgang J and Le Gros, Mark and Boudreau, Rosanne},
abstractNote = {Methods for determining metabolic properties of living cells through the uptake of semiconductor nanocrystals by cells. Generally the methods require a layer of neutral or hydrophilic semiconductor nanocrystals and a layer of cells seeded onto a culture surface and changes in the layer of semiconductor nanocrystals are detected. The observed changes made to the layer of semiconductor nanocrystals can be correlated to such metabolic properties as metastatic potential, cell motility or migration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {11}
}

Patent:

Save / Share:

Works referenced in this record:

Image analysis of Transwell assays in the assessment of invasion by malignant cell lines
journal, January 2002


In Vitro Characterization and Micromechanics of Tumor Cell Chemotactic Protrusion, Locomotion, and Extravasation
journal, March 2002

  • Dong, Cheng; Slattery, Margaret J.; Rank, Bradley M.
  • Annals of Biomedical Engineering, Vol. 30, Issue 3
  • DOI: 10.1114/1.1468889

Scanning electrochemical microscopy of living cells: Different redox activities of nonmetastatic and metastatic human breast cells
journal, August 2000

  • Liu, B.; Rotenberg, S. A.; Mirkin, M. V.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 18, p. 9855-9860
  • DOI: 10.1073/pnas.97.18.9855

Measurement of cell migration stimulated by interleukin 8: Use of ATP chemiluminescence
journal, July 1994


Effects of Transforming Growth Factor-beta on Human Pulmonary Adenocarcinoma Cell Adhesion, Motility, and Invasion In Vitro
journal, April 1992

  • Mooradian, D. L.; McCarthy, J. B.; Komanduri, K. V.
  • JNCI Journal of the National Cancer Institute, Vol. 84, Issue 7
  • DOI: 10.1093/jnci/84.7.523

Quantitative Methods for Scoring Cell Migration and Invasion in Filter-Based Assays
journal, November 1993


A sensitive screening assay for secreted motility-stimulating factors
journal, January 2000


An indirect bioluminescence method for the quantitative measurement of polymorphonuclear cell chemotaxis
journal, July 1991

  • Partsch, Gerald; Schwarzer, Christine
  • Luminescence: The Journal of Biological and Chemical Luminescence, Vol. 6, Issue 3, p. 159-167
  • DOI: 10.1002/bio.1170060305

Comparison of MTT and ATP-based assays for the measurement of viable cell number
journal, January 1995

  • Petty, Russell D.; Sutherland, Lesley A.; Hunter, Elizabeth M.
  • Luminescence: The Journal of Biological and Chemical Luminescence, Vol. 10, Issue 1, p. 29-34
  • DOI: 10.1002/bio.1170100105

A Modified and Convenient Method for Assessing Tumor Cell Invasion and Migration and Its Application to Screening for Inhibitors.
journal, January 1997

  • Saito, Ken-ichi; Oku, Tohru; Ata, Naomi
  • Biological & Pharmaceutical Bulletin, Vol. 20, Issue 4
  • DOI: 10.1248/bpb.20.345

Development of a novel human extracellular matrix for quantitation of the invasiveness of human cells
journal, April 1993


The influence of ethanol on cell membrane fluidity, migration, and invasion of murine melanoma cells
journal, July 1990

  • Silberman, Simone; McGarvey, Terence W.; Comrie, Eric
  • Experimental Cell Research, Vol. 189, Issue 1, p. 64-68
  • DOI: 10.1016/0014-4827(90)90257-B

The promotion of invasion through the basement membrane of cervical carcinoma cells by fibronectin as a chemoattractant
journal, May 1994


The phagokinetic tracks of 3T3 cells
journal, June 1977


Proliferative and migratory responses of astrocytes to in vitro injury
journal, January 2000


The Chemotactic Effect of Mixtures of Antibody and Antigen on Polymorphonuclear Leucocytes
journal, March 1962


Chemotaxis by a CNS macrophage, the microglia
journal, September 1990

  • Yao, J.; Harvath, L.; Gilbert, D. L.
  • Journal of Neuroscience Research, Vol. 27, Issue 1
  • DOI: 10.1002/jnr.490270106

Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998


Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998


Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum Dots
journal, September 2001

  • Gerion, Daniele; Pinaud, Fabien; Williams, Shara C.
  • The Journal of Physical Chemistry B, Vol. 105, Issue 37
  • DOI: 10.1021/jp0105488

Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules
journal, July 2001

  • Han, Mingyong; Gao, Xiaohu; Su, Jack Z.
  • Nature Biotechnology, Vol. 19, Issue 7, p. 631-635
  • DOI: 10.1038/90228

Synthesis of Soluble and Processable Rod-, Arrow-, Teardrop-, and Tetrapod-Shaped CdSe Nanocrystals
December 2000

  • Manna, Liberato; Scher, Erik C.; Alivisatos, A. Paul
  • Journal of the American Chemical Society, Vol. 122, Issue 51, p. 12700-12706
  • DOI: 10.1021/ja003055+

Selective Deposition of Proteins and Cells in Arrays of Microwells
journal, May 2001

  • Ostuni, Emanuele; Chen, Christopher S.; Ingber, Donald E.
  • Langmuir, Vol. 17, Issue 9
  • DOI: 10.1021/la001372o

Hydroxylated Quantum Dots as Luminescent Probes for in Situ Hybridization
journal, May 2001

  • Pathak, Srikant; Choi, Soo-Kyung; Arnheim, Norman
  • Journal of the American Chemical Society, Vol. 123, Issue 17
  • DOI: 10.1021/ja0058334

Shape control of CdSe nanocrystals
journal, March 2000

  • Peng, Xiaogang; Manna, Liberato; Yang, Weidong
  • Nature, Vol. 404, Issue 6773, p. 59-61
  • DOI: 10.1038/35003535

Comparison of a Novel Redox Dye Cell Growth Assay to the ATP Bioluminescence Assay
journal, July 1995

  • Squatrito, Robert C.; Connor, Joseph P.; Buller, Richard E.
  • Gynecologic Oncology, Vol. 58, Issue 1
  • DOI: 10.1006/gyno.1995.1190