Title: Sub-micrometer fluidic channel for measuring photon emitting entities

Abstract

A nanofluidic channel fabricated in fused silica with an approximately 500 nm square cross section was used to isolate, detect and identify individual quantum dot conjugates. The channel enables the rapid detection of every fluorescent entity in solution. A laser of selected wavelength was used to excite multiple species of quantum dots and organic molecules, and the emission spectra were resolved without significant signal rejection. Quantum dots were then conjugated with organic molecules and detected to demonstrate efficient multicolor detection. PCH was used to analyze coincident detection and to characterize the degree of binding. The use of a small fluidic channel to detect quantum dots as fluorescent labels was shown to be an efficient technique for multiplexed single molecule studies. Detection of single molecule binding events has a variety of applications including high throughput immunoassays.

Inventors:

Stavis, Samuel M; Edel, Joshua B; Samiee, Kevan T; Craighead, Harold G

Issue Date:

2014-11-18

Research Org.:

Cornell Research Foundation, Ithaca, NY (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1163985

Patent Number(s):

8890323

Application Number:

12/716,087

Assignee:

Cornell Research Foundation (Ithaca, NY)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K49/0056 - {Peptides, proteins, polyamino acids}
A61K49/0067 - {quantum dots, fluorescent nanocrystals}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y10/00 - Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
B82Y15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
B82Y20/00 - Nanooptics, e.g. quantum optics or photonic crystals
B82Y30/00 - Nanotechnology for materials or surface science, e.g. nanocomposites
B82Y5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/588 - {with semiconductor nanocrystal label, e.g. quantum dots}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S438/962 - Quantum dots and lines
Y10S977/70 - Nanostructure
Y10S977/774 - Exhibiting three-dimensional carrier confinement, e.g. quantum dots

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DOE Contract Number:  

FG02-99ER62809

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSICS; 36 MATERIALS SCIENCE