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Title: Nanolipoprotein particles and related compositions, methods and systems


Functionalized nanolipoprotein particle presenting an anchor substrate compound for binding with a corresponding anchor compound presented on a target molecule, and related compositions methods and systems.

; ; ;
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
OSTI Identifier:
Patent Number(s):
Application Number:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
DOE Contract Number:  
Resource Type:
Resource Relation:
Patent File Date: 2009 May 20
Country of Publication:
United States

Citation Formats

Hoeprich, Paul D, Fischer, Nicholas O, Mason, Peter W, and Blanchette, Craig D. Nanolipoprotein particles and related compositions, methods and systems. United States: N. p., 2014. Web.
Hoeprich, Paul D, Fischer, Nicholas O, Mason, Peter W, & Blanchette, Craig D. Nanolipoprotein particles and related compositions, methods and systems. United States.
Hoeprich, Paul D, Fischer, Nicholas O, Mason, Peter W, and Blanchette, Craig D. Tue . "Nanolipoprotein particles and related compositions, methods and systems". United States.
title = {Nanolipoprotein particles and related compositions, methods and systems},
author = {Hoeprich, Paul D and Fischer, Nicholas O and Mason, Peter W and Blanchette, Craig D},
abstractNote = {Functionalized nanolipoprotein particle presenting an anchor substrate compound for binding with a corresponding anchor compound presented on a target molecule, and related compositions methods and systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {11}

Works referenced in this record:

Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid Interfaces
journal, September 1994

Modulation of human erg K+ channel gating by activation of a G protein-coupled receptor and protein kinase C
journal, September 1998

Assembly of single bacteriorhodopsin trimers in bilayer nanodiscs
journal, June 2006

Reconstitution and Imaging of a Membrane Protein in a Nanometer-Size Phospholipid Bilayer
journal, September 1998

Self-assembly of single integral membrane proteins into soluble nanoscale phospholipid bilayers
journal, November 2003

Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins
journal, August 2002

Bacterial Rhodopsin: Evidence for a New Type of Phototrophy in the Sea
journal, September 2000

Specific targeting of a lipophilic prodrug of iododeoxyuridine to parenchymal liver cells using lactosylated reconstituted high density lipoprotein particles
journal, July 1996

Specific Interaction and Two-Dimensional Crystallization of Histidine Tagged Yeast RNA Polymerase I on Nickel-Chelating Lipids
journal, March 1998

Atomic force microscopy differentiates discrete size distributions between membrane protein containing and empty nanolipoprotein particles
journal, March 2009

Characterization and Purification of Polydisperse Reconstituted Lipoproteins and Nanolipoprotein Particles
journal, July 2009

Quantifying size distributions of nanolipoprotein particles with single-particle analysis and molecular dynamic simulations
journal, April 2008

Nanodiscs separate chemoreceptor oligomeric states and reveal their signaling properties
journal, July 2006

Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor
journal, August 2008

Osmotic shrinkage of giant egg-lecithin vesicles
journal, April 1981

Chemoselective Attachment of Biologically Active Proteins to Surfaces by Expressed Protein Ligation and Its Application for “Protein Chip” Fabrication
journal, November 2004

  • Camarero, Julio A.; Kwon, Youngeun; Coleman, Matthew A.
  • Journal of the American Chemical Society, Vol. 126, Issue 45, p. 14730-14731

Cell-free Co-expression of Functional Membrane Proteins and Apolipoprotein, Forming Soluble Nanolipoprotein Particles
journal, July 2008

CpG oligodeoxynucleotides as DNA adjuvants in vertebrates and their applications in immunotherapy
journal, October 2006

Attaching histidine-tagged peptides and proteins to lipid-based carriers through use of metal-ion-chelating lipids
journal, December 2002

Stability of pressure-extruded liposomes made from archaeobacterial ether lipids
journal, November 1994

Different Apolipoproteins Impact Nanolipoprotein Particle Formation
journal, November 2007

  • Chromy, Brett A.; Arroyo, Erin; Blanchette, Craig D.
  • Journal of the American Chemical Society, Vol. 129, Issue 46, p. 14348-14354

Asp 46 can substitute for Asp 96 as the Schiff base proton donor in bacteriorhodopsin
journal, November 1995

Surface changes induced by osmotic shrinkage on large unilamellar vesicles
journal, November 1996

Dopamine tone regulates D1 receptor trafficking and delivery in striatal neurons in dopamine transporter-deficient mice
journal, February 2000

Immobilization of Active Hydrogenases by Encapsulation in Polymeric Porous Gels
journal, October 2005

Immobilization of His-Tagged Proteins on Nickel-Chelating Nanolipoprotein Particles
journal, March 2009

Conjugation to Nickel-Chelating Nanolipoprotein Particles Increases the Potency and Efficacy of Subunit Vaccines to Prevent West Nile Encephalitis
journal, June 2010

IMMUNOLOGY: The Shape of Things to Come
journal, June 2007

Carboxyl-Terminal Domain of Human Apolipoprotein E: Expression, Purification, and Crystallization
journal, November 1999

Electron microscopic study on reassembly of plasma high density apoprotein with various lipids
journal, November 1971

Charge‐reduced nano electrospray ionization combined with differential mobility analysis of peptides, proteins, glycoproteins, noncovalent protein complexes and viruses
journal, September 2001

  • Bacher, Gerold; Szymanski, Wladyslaw W.; Kaufman, Stanley L.
  • Journal of Mass Spectrometry, Vol. 36, Issue 9, p. 1038-1052

Molecular cloning of a gene encoding the histamine H2 receptor.
journal, January 1991

Hydrogen Production under Aerobic Conditions by Membrane-Bound Hydrogenases from Ralstonia Species
journal, August 2008

Adjuvants for human vaccines—current status, problems and future prospects
journal, January 1995

Complex of Human Apolipoprotein C-1 with Phospholipid:  Thermodynamic or Kinetic Stability?
journal, June 2002

Pharmaceutical analysis of synthetic lipid A-based vaccine adjuvants in poly (d,l-lactic-co-glycolic acid) nanoparticle formulations
journal, August 2007

Corticotropin Releasing Factor (CRF) Receptor Signaling in the Central Nervous System: New Molecular Targets
journal, August 2006

Energy-Converting [NiFe] Hydrogenases from Archaea and Extremophiles: Ancestors of Complex I
journal, February 2004

Click Chemistry, A Powerful Tool for Pharmaceutical Sciences
journal, May 2008

Influence of liposome charge and composition on their interaction with human blood serum proteins
journal, January 1993

  • Hernández-Caselles, Trinidad; Villalaín, José; Gómez-Fernández, Juan C.
  • Molecular and Cellular Biochemistry, Vol. 120, Issue 2, p. 119-126

Fabrication of biomolecule–copolymer hybrid nanovesicles as energy conversion systems
journal, November 2005

G-Protein-Coupled Receptor Microarrays for Multiplexed Compound Screening
journal, June 2006

Comparison of the Dynamics of the Primary Events of Bacteriorhodopsin in Its Trimeric and Monomeric States
journal, September 2002

Indium-111 Labeling of Low Density Lipoproteins with the DTPA−Bis(stearylamide):  Evaluation as a Potential Radiopharmaceutical for Tumor Localization
journal, January 1996

Structural Basis for Thermal Stability of Human Low-Density Lipoprotein
journal, March 2005

Thermotolerant Hydrogenases: Biological Diversity, Properties, and Biotechnological Applications
journal, January 2008

Functional Cell-free Synthesis of a Seven Helix Membrane Protein: In situ Insertion of Bacteriorhodopsin into Liposomes
journal, August 2007

Insertion of Membrane Proteins into Discoidal Membranes Using a Cell-Free Protein Expression Approach
journal, August 2008

Cell-free expression as an emerging technique for the large scale production of integral membrane protein
journal, September 2006

High level cell-free expression and specific labeling of integral membrane proteins
journal, February 2004

The growing impact of click chemistry on drug discovery
journal, December 2003

Two-Dimensional Crystallization of Histidine-Tagged, HIV-1 Reverse Transcriptase Promoted by a Novel Nickel-Chelating Lipid
journal, September 1994

Novel applications of liposomes
journal, July 1998

Functional reconstitution of β 2 -adrenergic receptors utilizing self-assembling Nanodisc technology
journal, May 2006

Mechanical properties and stability measurement of cholesterol-containing liposome on mica by atomic force microscopy
journal, October 2004

Conformational Reorganization of the Four-helix Bundle of Human Apolipoprotein E in Binding to Phospholipid
journal, May 2000

Large-scale profiling of protein palmitoylation in mammalian cells
journal, January 2009

The Vaccine Adjuvant Monophosphoryl Lipid A as a TRIF-Biased Agonist of TLR4
journal, June 2007

ACTH, α-MSH, and control of cortisol release: cloning, sequencing, and functional expression of the melanocortin-2 and melanocortin-5 receptor in C yprinus carpio
journal, September 2005

[FeFe] hydrogenases and their evolution: a genomic perspective
journal, March 2007

Functional Characterization of Apolipoprotein E Isoforms Overexpressed in Escherichia coli
journal, July 1999

Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins
journal, February 2007

A Potent Adjuvant Monophosphoryl Lipid A Triggers Various Immune Responses, but Not Secretion of IL-1β or Activation of Caspase-1
journal, January 2006

In-depth analysis of the membrane and cytosolic proteome of red blood cells
journal, August 2006

Taking toll: lipid A mimetics as adjuvants and immunomodulators
journal, October 2002

Topology prediction of membrane proteins
journal, February 1996

The Effects of Deleting the Mouse Neurotensin Receptor NTR1 on Central and Peripheral Responses to Neurotensin
journal, January 2002

Comparison of Multiplexed Techniques for Detection of Bacterial and Viral Proteins
journal, August 2004

Two-Dimensional Streptavidin Crystals on Giant Lipid Bilayer Vesicles
journal, May 2002

Different G protein-coupled receptor kinases govern G protein and β-arrestin-mediated signaling of V2 vasopressin receptor
journal, January 2005

Recombinant lipoproteins: lipoprotein-like lipid particles for drug targeting
journal, April 2001

Generation of immunoliposomes using recombinant single-chain Fv fragments bound to Ni-NTA-liposomes
journal, August 2005

In vitro characterization of binding and stability of single-chain Fv Ni-NTA-liposomes
journal, January 2006

Chemistry: The photon trap
journal, March 2008

Purification and Characterization of a Membrane-Bound Hydrogenase from the Hyperthermophilic Archaeon Pyrococcus furiosus
journal, June 2000

A simple energy-conserving system: Proton reduction coupled to proton translocation
journal, June 2003

Laboratory scale structural genomics
journal, March 2004

Molecular Dynamics Simulations of Discoidal Bilayers Assembled from Truncated Human Lipoproteins
journal, January 2005

Chemical modification of hemoglobins: a study of conformation restraint by internal bridging.
journal, August 1966

Membrane-Mimetic Films of Asymmetric Phosphatidylcholine Lipid Bolaamphiphiles
journal, January 2006

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
journal, January 2003

Peptide stabilized amphotericin B nanodisks
journal, April 2007

Induction of tumor necrosis factor-α in solid tumor region by the orally administered synthetic muramyl dipeptide analogue, romurtide
journal, January 2001

Vaccine manufacturing: challenges and solutions
journal, November 2006

Occurrence, Classification, and Biological Function of Hydrogenases:  An Overview
journal, October 2007

Investigating and Exploiting the Electrocatalytic Properties of Hydrogenases
journal, October 2007

From The Cover: Electrocatalytic hydrogen oxidation by an enzyme at high carbon monoxide or oxygen levels
journal, October 2005

CpG DNA induces stronger immune responses with less toxicity than other adjuvants
journal, March 2000

In vitro hydrogen production by glucose dehydrogenase and hydrogenase
journal, July 1996

Enzymatic production of biohydrogen: Biotechnology
journal, June 2000

High yield cell-free production of integral membrane proteins without refolding or detergents
journal, May 2008

High-Yield Hydrogen Production from Starch and Water by a Synthetic Enzymatic Pathway
journal, May 2007

Immunostimulatory DNA as adjuvant: efficacy of phosphodiester CpG oligonucleotides is enhanced by 3′ sequence modifications
journal, February 2003