Title: Metabolic evolution of Escherichia coli strains that produce organic acids

Abstract

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

Inventors:

Grabar, Tammy; Gong, Wei; Yocum, R Rogers

Issue Date:

2014-10-28

Research Org.:

Myriant Corporation, Quincy, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1162117

Patent Number(s):

8871489

Application Number:

13/394,176

Assignee:

Myriant Corporation (Quincy, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/245 - Escherichia

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/54 - Acetic acid

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DOE Contract Number:  

EE0002878/001

Resource Type:

Patent

Resource Relation:

Patent File Date: 2010 Nov 17

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES