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Title: Metabolic evolution of Escherichia coli strains that produce organic acids

Abstract

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.

Inventors:
; ;
Issue Date:
Research Org.:
Myriant Corporation, Quincy, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1162117
Patent Number(s):
8871489
Application Number:
13/394,176
Assignee:
Myriant Corporation (Quincy, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
EE0002878/001
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Nov 17
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Grabar, Tammy, Gong, Wei, and Yocum, R Rogers. Metabolic evolution of Escherichia coli strains that produce organic acids. United States: N. p., 2014. Web.
Grabar, Tammy, Gong, Wei, & Yocum, R Rogers. Metabolic evolution of Escherichia coli strains that produce organic acids. United States.
Grabar, Tammy, Gong, Wei, and Yocum, R Rogers. Tue . "Metabolic evolution of Escherichia coli strains that produce organic acids". United States. https://www.osti.gov/servlets/purl/1162117.
@article{osti_1162117,
title = {Metabolic evolution of Escherichia coli strains that produce organic acids},
author = {Grabar, Tammy and Gong, Wei and Yocum, R Rogers},
abstractNote = {This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {10}
}

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