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Title: Microorganisms for producing organic acids

Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.
Inventors:
;
Issue Date:
OSTI Identifier:
1159933
Assignee:
Wisconsin Alumni Research Foundation (Madison, WI) CHO
Patent Number(s):
8,846,354
Application Number:
14/200,747
Contract Number:
FC02-07ER64494
Research Org:
Wisconsin Alumni Research Foundation, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Works referenced in this record:

Basic local alignment search tool
journal, October 1990
  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Energy biotechnology with cyanobacteria
journal, June 2009
  • Angermayr, S. Andreas; Hellingwerf, Klaas J.; Lindblad, Peter
  • Current Opinion in Biotechnology, Vol. 20, Issue 3, p. 257-263
  • DOI: 10.1016/j.copbio.2009.05.011

Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
journal, November 2009
  • Atsumi, Shota; Higashide, Wendy; Liao, James C.
  • Nature Biotechnology, Vol. 27, Issue 12, p. 1177-1180
  • DOI: 10.1038/nbt.1586

Technology development for the production of biobased products from biorefinery carbohydrates—the US Department of Energy’s “Top 10” revisited
journal, January 2010
  • Bozell, Joseph J.; Petersen, Gene R.
  • Green Chemistry, Vol. 12, Issue 4, p. 539-554
  • DOI: 10.1039/B922014C

Engineering cyanobacteria to generate high-value products
journal, February 2011

Paraquat and the exacerbation of oxygen toxicity
journal, May 1979

Participation of a medium chain acyl-CoA synthetase in glycine conjugation of the benzoic acid derivatives with the electron-donating groups
journal, March 1996

Manufacturing Molecules Through Metabolic Engineering
journal, December 2010

Development of suitable photobioreactors for CO2 sequestration addressing global warming using green algae and cyanobacteria
journal, April 2011
  • Kumar, Kanhaiya; Dasgupta, Chitralekha Nag; Nayak, Bikram
  • Bioresource Technology, Vol. 102, Issue 8, p. 4945-4953
  • DOI: 10.1016/j.biortech.2011.01.054

Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism
journal, January 2010

The binding of propionyl-CoA and carboxymethyl-CoA to Escherichia coli citrate synthase
journal, July 1995
  • Man, Wai-Jin; Li, Yan; David O'Connor, C.
  • Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1250, Issue 1, p. 69-75
  • DOI: 10.1016/0167-4838(95)00044-U

Metabolic engineering for the microbial production of 1,3-propanediol
journal, October 2003

A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970

Ellman's reagent: 5,5′-dithiobis(2-nitrobenzoic acid)—a reexamination
journal, April 1979
  • Riddles, Peter W.; Blakeley, Robert L.; Zerner, Burt
  • Analytical Biochemistry, Vol. 94, Issue 1, p. 75-81
  • DOI: 10.1016/0003-2697(79)90792-9

Another explanation for the toxicity of fermentation acids at low pH: anion accumulation versus uncoupling
journal, November 1992

Comparison of biosequences
journal, December 1981

Determinations of dimethylsulphoniopropionate (DMSP) lyase activity using headspace analysis of dimethylsulphide (DMS)
journal, August 2000

Physiological aspects of the production and conversion of DMSP in marine algae and higher plants
journal, August 2000

Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes
journal, May 2010
  • Warnecke, T. E.; Lynch, M. D.; Karimpour-Fard, A.
  • Metabolic Engineering, Vol. 12, Issue 3, p. 241-250
  • DOI: 10.1016/j.ymben.2009.12.002