DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: L-arabinose fermenting yeast

Abstract

An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.

Inventors:
; ; ; ; ;
Issue Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1159899
Patent Number(s):
8841090
Application Number:
13/744,023
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 60 APPLIED LIFE SCIENCES

Citation Formats

Zhang, Min, Singh, Arjun, Suominen, Pirkko, Knoshaug, Eric, Franden, Mary Ann, and Jarvis, Eric. L-arabinose fermenting yeast. United States: N. p., 2014. Web.
Zhang, Min, Singh, Arjun, Suominen, Pirkko, Knoshaug, Eric, Franden, Mary Ann, & Jarvis, Eric. L-arabinose fermenting yeast. United States.
Zhang, Min, Singh, Arjun, Suominen, Pirkko, Knoshaug, Eric, Franden, Mary Ann, and Jarvis, Eric. Tue . "L-arabinose fermenting yeast". United States. https://www.osti.gov/servlets/purl/1159899.
@article{osti_1159899,
title = {L-arabinose fermenting yeast},
author = {Zhang, Min and Singh, Arjun and Suominen, Pirkko and Knoshaug, Eric and Franden, Mary Ann and Jarvis, Eric},
abstractNote = {An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. A yeast strain engineered to metabolize arabinose through a novel pathway is also disclosed. Methods of producing ethanol include utilizing these modified yeast strains.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 23 00:00:00 EDT 2014},
month = {Tue Sep 23 00:00:00 EDT 2014}
}

Works referenced in this record:

L-arabinose fermenting yeast
patent, December 2010


L-arabinose fermenting yeast
patent, February 2013


Engineering fungi for the utilisation of l-arabinose
patent-application, October 2003


Modified yeast consuming L-arabinose
patent-application, June 2005


Basic local alignment search tool
journal, October 1990


Cloning and characterization of the gene encoding a high-affinity maltose transporter from
journal, January 2004


When blind is better: Protein design by evolution
journal, July 1998


A Modified Saccharomyces cerevisiae Strain That Consumes L-Arabinose and Produces Ethanol
journal, July 2003


Glucose uptake in Kluyveromyces lactis: role of the HGT1 gene in glucose transport.
journal, October 1996


Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990


A new dominant hybrid selective marker for higher eukaryotic cells
journal, July 1981


ULBPs, Novel MHC Class I–Related Molecules, Bind to CMV Glycoprotein UL16 and Stimulate NK Cytotoxicity through the NKG2D Receptor
journal, February 2001


DNA shuffling of a family of genes from diverse species accelerates directed evolution
journal, January 1998


Characterization of the putative maltose transporters encoded by YDL247w and YJR160c
journal, January 2002


Genome evolution in yeasts
journal, July 2004


Computational protein engineering
journal, July 1998


Extraction and Characterization of Hemicellulose from the Corn Fiber Produced by Corn Wet-Milling Processes
journal, July 1998


DMSO-enhanced Whole cell yeast transformation
journal, January 1991


The hexose transporter family of Saccharomyces cerevisiae
journal, November 1996


The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
journal, June 1988


Trends in the Development of Baculovirus Expression Vectors
journal, January 1988


Arabinose utilization by xylose-fermenting yeasts and fungi
journal, March 1994


Metabolic Engineering of Saccharomyces cerevisiae
journal, March 2000


The Missing Link in the Fungal l -Arabinose Catabolic Pathway, Identification of the l -Xylulose Reductase Gene ,
journal, May 2002


Production of L -arabitol from L -arabinose by Candida entomaea and Pichia guilliermondii
journal, April 1996


The neighbor-joining method: a new method for reconstructing phylogenetic trees.
journal, July 1987


Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells
journal, October 1984


Expression of E. coli araBAD operon encoding enzymes for metabolizing L-arabinose in Saccharomyces cerevisiae
journal, January 2001


Toward controlling gene expression at will: Selection and design of zinc finger domains recognizing each of the 5'-GNN-3' DNA target sequences
journal, March 1999


Effect of surface modification using various acids on electrodeposition of lithium
journal, June 1995


Comparison of biosequences
journal, December 1981


d-Xylose Transport by Candida succiphila and Kluyveromyces marxianus
book, January 2003


Cloning and characterization of three genes (SUT1-3 ) encoding glucose transporters of the yeast Pichia stipitis
journal, February 1999


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


Molecular evolution by staggered extension process (StEP) in vitro recombination
journal, March 1998