Process and genes for expression and overexpression of active [FeFe] hydrogenases
Abstract
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.
- Inventors:
- Issue Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1159827
- Patent Number(s):
- 8835153
- Application Number:
- 11/573,035
- Assignee:
- Alliance for Sustainable Energy, LLC (Golden, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-99GO10337
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2006 Feb 28
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, and Smolinski, Sharon L. Process and genes for expression and overexpression of active [FeFe] hydrogenases. United States: N. p., 2014.
Web.
Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, & Smolinski, Sharon L. Process and genes for expression and overexpression of active [FeFe] hydrogenases. United States.
Seibert, Michael, King, Paul W, Ghirardi, Maria Lucia, Posewitz, Matthew C, and Smolinski, Sharon L. Tue .
"Process and genes for expression and overexpression of active [FeFe] hydrogenases". United States. https://www.osti.gov/servlets/purl/1159827.
@article{osti_1159827,
title = {Process and genes for expression and overexpression of active [FeFe] hydrogenases},
author = {Seibert, Michael and King, Paul W and Ghirardi, Maria Lucia and Posewitz, Matthew C and Smolinski, Sharon L},
abstractNote = {A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {9}
}
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Works referenced in this record:
Purification and characterization of Desulfovibrio vulgaris (Hildenborough) hydrogenase expressed in Escherichia coli
journal, January 1987
- Voordouw, Gerrit; Hagen, Wilfred R.; Kruse-Wolters, K. Margreet
- European Journal of Biochemistry, Vol. 162, Issue 1
Methods and Compositions for Evolving Hydrogenase Genes
patent-application, October 2004
- Dillion, Harrison
- US Patent Application 10/411910; 20040209256
Discovery of Two Novel Radical S -Adenosylmethionine Proteins Required for the Assembly of an Active [Fe] Hydrogenase
journal, April 2004
- Posewitz, Matthew C.; King, Paul W.; Smolinski, Sharon L.
- Journal of Biological Chemistry, Vol. 279, Issue 24
Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12.
journal, August 1989
- Stoker, K.; Reijnders, W. N.; Oltmann, L. F.
- Journal of Bacteriology, Vol. 171, Issue 8
Iron-Dependent Hydrogenases of Entamoeba histolytica and Giardia lamblia: Activity of the Recombinant Entamoebic Enzyme and Evidence for Lateral Gene Transfer
journal, February 2003
- Nixon, Julie E. J.; Field, Jessica; McArthur, Andrew G.
- The Biological Bulletin, Vol. 204, Issue 1
Characterization of a [2Fe-2S] protein encoded in the iron-hydrogenase operon of Thermotoga maritima
journal, February 2003
- Pan, Guangliang; Menon, Angeli Lal; Adams, Michael W. W.
- JBIC Journal of Biological Inorganic Chemistry, Vol. 8, Issue 4
Functional Studies of [FeFe] Hydrogenase Maturation in an Escherichia coli Biosynthetic System
journal, March 2006
- King, P. W.; Posewitz, M. C.; Ghirardi, M. L.
- Journal of Bacteriology, Vol. 188, Issue 6
Heterologous expression of clostridial hydrogenase in the cyanobacterium Synechococcus PCC7942
journal, February 2000
- Asada, Yasuo; Koike, Yoji; Schnackenberg, Jörg
- Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1490, Issue 3
Isolation of Genes Required for Hydrogenase Synthesis in Escherichia coli
journal, December 1987
- Chaudhuri, A.; Krasna, A. I.
- Microbiology, Vol. 133, Issue 12
Classification and phylogeny of hydrogenases
journal, August 2001
- Vignais, Paulette M.; Billoud, Bernard; Meyer, Jacques
- FEMS Microbiology Reviews, Vol. 25, Issue 4, p. 455-501
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
journal, February 1990
- Kindle, K. L.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 3
Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii
journal, June 1993
- Happe, Thomas; Naber, J. Dirk
- European Journal of Biochemistry, Vol. 214, Issue 2
Incorporation of Iron and Sulfur from NifB Cofactor into the Iron-Molybdenum Cofactor of Dinitrogenase
journal, November 1995
- Allen, Ronda M.; Chatterjee, Ranjini; Ludden, Paul W.
- Journal of Biological Chemistry, Vol. 270, Issue 45
Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions: Expression of a second [Fe]-hydrogenase in C. reinhardtii
journal, June 2003
- Forestier, Marc; King, Paul; Zhang, Liping
- European Journal of Biochemistry, Vol. 270, Issue 13
Mutational analysis and characterization of the Escherichia coli hya operon, which encodes [NiFe] hydrogenase 1.
journal, August 1991
- Menon, N. K.; Robbins, J.; Wendt, J. C.
- Journal of Bacteriology, Vol. 173, Issue 15