Nucleic acid compositions and the encoding proteins
Abstract
The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.
- Inventors:
- Issue Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1154933
- Patent Number(s):
- 8822179
- Application Number:
- 13/350,858
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
- DOE Contract Number:
- FG-02GO12026; FC36-99GO10476; FC36-00GO10594
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D., and St. John, Franz J. Nucleic acid compositions and the encoding proteins. United States: N. p., 2014.
Web.
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D., & St. John, Franz J. Nucleic acid compositions and the encoding proteins. United States.
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D., and St. John, Franz J. Tue .
"Nucleic acid compositions and the encoding proteins". United States. https://www.osti.gov/servlets/purl/1154933.
@article{osti_1154933,
title = {Nucleic acid compositions and the encoding proteins},
author = {Preston, III, James F. and Chow, Virginia and Nong, Guang and Rice, John D. and St. John, Franz J.},
abstractNote = {The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {9}
}
Works referenced in this record:
Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4
journal, February 2000
- Takami, H.; Krulwich, T. A.
- Extremophiles, Vol. 4, Issue 1
Bacteria engineered for fuel ethanol production: current status
journal, December 2003
- Dien, B. S.; Cotta, M. A.; Jeffries, T. W.
- Applied Microbiology and Biotechnology, Vol. 63, Issue 3, p. 258-266