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Title: Platform for immobilization and observation of subcellular processes

Abstract

A method of immobilizing matter for imaging that includes providing an array of nanofibers and directing matter to the array of the nanofibers. The matter is immobilized when contacting at least three nanofibers of the array of nanofibers simultaneously. Adjacent nanofibers in the array of nanofibers may be separated by a pitch as great as 100 microns. The immobilized matter on the array of nanofibers may then be imaged. In some examples, the matter may be cell matter, such as protoplasts.

Inventors:
; ;
Issue Date:
Research Org.:
ORNL (Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1151763
Patent Number(s):
8,815,780
Application Number:
13/369,849
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
DOE Contract Number:  
AC05-000R22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

McKnight, Timothy E., Kalluri, Udaya C., and Melechko, Anatoli V. Platform for immobilization and observation of subcellular processes. United States: N. p., 2014. Web.
McKnight, Timothy E., Kalluri, Udaya C., & Melechko, Anatoli V. Platform for immobilization and observation of subcellular processes. United States.
McKnight, Timothy E., Kalluri, Udaya C., and Melechko, Anatoli V. Tue . "Platform for immobilization and observation of subcellular processes". United States. https://www.osti.gov/servlets/purl/1151763.
@article{osti_1151763,
title = {Platform for immobilization and observation of subcellular processes},
author = {McKnight, Timothy E. and Kalluri, Udaya C. and Melechko, Anatoli V.},
abstractNote = {A method of immobilizing matter for imaging that includes providing an array of nanofibers and directing matter to the array of the nanofibers. The matter is immobilized when contacting at least three nanofibers of the array of nanofibers simultaneously. Adjacent nanofibers in the array of nanofibers may be separated by a pitch as great as 100 microns. The immobilized matter on the array of nanofibers may then be imaged. In some examples, the matter may be cell matter, such as protoplasts.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {8}
}

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Works referenced in this record:

Culture and chemical-induced fusion of tobacco mesophyll protoplasts in a microfluidic device
journal, October 2010


Tobacco protoplast culture in a polydimethylsiloxane-based microfluidic channel
journal, May 2006