Plant isoflavone and isoflavanone O-methyltransferase genes
Abstract
The invention provides enzymes that encode O-methyltransferases (OMTs) from Medicago truncatula that allow modification to plant (iso)flavonoid biosynthetic pathways. In certain aspects of the invention, the genes encoding these enzymes are provided. The invention therefore allows the modification of plants for isoflavonoid content. Transgenic plants comprising such enzymes are also provided, as well as methods for improving disease resistance in plants. Methods for producing food and nutraceuticals, and the resulting compositions, are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1150638
- Patent Number(s):
- 8809627
- Application Number:
- 11/840,032
- Assignee:
- The Samuel Roberts Noble Foundation (Ardmore, OK)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Broeckling, Bettina E., Liu, Chang-Jun, and Dixon, Richard A. Plant isoflavone and isoflavanone O-methyltransferase genes. United States: N. p., 2014.
Web.
Broeckling, Bettina E., Liu, Chang-Jun, & Dixon, Richard A. Plant isoflavone and isoflavanone O-methyltransferase genes. United States.
Broeckling, Bettina E., Liu, Chang-Jun, and Dixon, Richard A. Tue .
"Plant isoflavone and isoflavanone O-methyltransferase genes". United States. https://www.osti.gov/servlets/purl/1150638.
@article{osti_1150638,
title = {Plant isoflavone and isoflavanone O-methyltransferase genes},
author = {Broeckling, Bettina E. and Liu, Chang-Jun and Dixon, Richard A.},
abstractNote = {The invention provides enzymes that encode O-methyltransferases (OMTs) from Medicago truncatula that allow modification to plant (iso)flavonoid biosynthetic pathways. In certain aspects of the invention, the genes encoding these enzymes are provided. The invention therefore allows the modification of plants for isoflavonoid content. Transgenic plants comprising such enzymes are also provided, as well as methods for improving disease resistance in plants. Methods for producing food and nutraceuticals, and the resulting compositions, are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {8}
}
Works referenced in this record:
Isoflavonoid methylation enzyme
patent, April 2005
- Dixon, Richard A.; He, Xian
- US Patent Document 6,878,859
A new, structurally nonredundant, diverse data set of protein-protein interfaces and its implications
journal, April 2004
- Keskin, Ozlem; Tsai, Chung-Jung; Wolfson, Haim
- Protein Science, Vol. 13, Issue 4, p. 1043-1055
Protein tolerance to random amino acid change
journal, June 2004
- Guo, H. H.; Choe, J.; Loeb, L. A.
- Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
Additivity of mutational effects in proteins
journal, September 1990
- Wells, James A.
- Biochemistry, Vol. 29, Issue 37, p. 8509-8517
Characterization of Phenylpropene O -Methyltransferases from Sweet Basil : Facile Change of Substrate Specificity and Convergent Evolution within a Plant
journal, February 2002
- Gang, David R.; Lavid, Noa; Zubieta, Chloe
- The Plant Cell, Vol. 14, Issue 2
Predicting the substrates of cloned plant O-methyltransferases
journal, January 2002
- Schröder, Gudrun; Wehinger, Elke; Schröder, Joachim
- Phytochemistry, Vol. 59, Issue 1, p. 1-8
cDNA Cloning and Biochemical Characterization of S-Adenosyl-l-Methionine: 2,7,4′-Trihydroxyisoflavanone 4′-O-Methyltransferase, a Critical Enzyme of the Legume Isoflavonoid Phytoalexin Pathway
journal, February 2003
- Akashi, Tomoyoshi; Sawada, Yuji; Shimada, Norimoto
- Plant and Cell Physiology, Vol. 44, Issue 2
Generation of 7137 Non-redundant Expressed Sequence Tags from a Legume, Lotus japonicus
journal, January 2000
- Asamizu, E.; Nakamura, Y.; Sato, S.
- DNA Research, Vol. 7, Issue 2
Combinatorial biochemistry in plants: the case of O-methyltransferases
journal, January 2001
- Frick, Susanne; Ounaroon, Anan; Kutchan, Toni M.
- Phytochemistry, Vol. 56, Issue 1, p. 1-4
Affinity Chromatography, Substrate/Product Specificity, and Amino Acid Sequence Analysis of an IsoflavoneO-Methyltransferase from Alfalfa (Medicago sativaL.)
journal, December 1996
- He, Xian-Zhi; Dixon, Richard A.
- Archives of Biochemistry and Biophysics, Vol. 336, Issue 1
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase
journal, January 1998
- He, Xian-Zhi; Reddy, J. T.; Dixon, Richard A.
- Plant Molecular Biology, Vol. 36, Issue 1, p. 43-54
Conserved sequence motifs in plant S-adenosyl-L-methionine-dependent methyltransferases
journal, January 1998
- Joshi, Chandrashekhar P.; Chiang, Vincent L.
- Plant Molecular Biology, Vol. 37, Issue 4, p. 663-674
Regiospecific methylation of naringenin to ponciretin by soybean O-methyltransferase expressed in Escherichia coli
journal, September 2005
- Kim, Dae Hwan; Kim, Bong-Gyu; Lee, Youngshim
- Journal of Biotechnology, Vol. 119, Issue 2, p. 155-162
Flavonoid methylation: a novel 4$prime;-O-methyltransferase from Catharanthus roseus, and evidence that partially methylated flavanones are substrates of four different flavonoid dioxygenases
journal, April 2004
- Schroder, G.
- Phytochemistry, Vol. 65, Issue 8
Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula
journal, December 2004
- Suzuki, Hideyuki; Reddy, M. S. Srinivasa; Naoumkina, Marina
- Planta, Vol. 220, Issue 5