Title: Combined hairpin-antisense compositions and methods for modulating expression

Abstract

A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Inventors:

Shanklin, John; Nguyen, Tam

Issue Date:

2014-08-05

Research Org.:

Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1150002

Patent Number(s):

8796442

Application Number:

12/052,658

Assignee:

Brookhaven Science Associates, LLC (Upton, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N2310/3519 - Fusion with another nucleic acid
C12N2310/53 - partially self-complementary or closed
C12N15/8247 - {involving modified lipid metabolism, e.g. seed oil composition}
C12N2310/11 - Antisense
C12N15/8218 - {Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]}
C12N15/111 - {General methods applicable to biologically active non-coding nucleic acids}
C12N2310/14 - interfering N.A.

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DOE Contract Number:  

AC02-98CH10886

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES