Microorganisms having enhanced tolerance to inhibitors and stress
Abstract
The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.
- Inventors:
- Issue Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1149918
- Patent Number(s):
- 8790902
- Application Number:
- 13/589,709
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Brown, Steven D., and Yang, Shihui. Microorganisms having enhanced tolerance to inhibitors and stress. United States: N. p., 2014.
Web.
Brown, Steven D., & Yang, Shihui. Microorganisms having enhanced tolerance to inhibitors and stress. United States.
Brown, Steven D., and Yang, Shihui. Tue .
"Microorganisms having enhanced tolerance to inhibitors and stress". United States. https://www.osti.gov/servlets/purl/1149918.
@article{osti_1149918,
title = {Microorganisms having enhanced tolerance to inhibitors and stress},
author = {Brown, Steven D. and Yang, Shihui},
abstractNote = {The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {7}
}
Works referenced in this record:
Identification of yeast deletion strains that are hypersensitive to brefeldin A or monensin, two drugs that affect intracellular transport
journal, January 2001
- Mur�n, Eva; �yen, Mattias; Barmark, Gunilla
- Yeast, Vol. 18, Issue 2
Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation
journal, December 2006
- Tanaka, Fumiko; Ando, Akira; Nakamura, Toshihide
- Food Microbiology, Vol. 23, Issue 8, p. 717-728
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998
- Broun, Pierre; Shanklin, John; Whittle, Ed
- Science, Vol. 282, Issue 5392, p. 1315-1317
Practical limits of function prediction
journal, January 2000
- Devos, Damien; Valencia, Alfonso
- Proteins: Structure, Function, and Genetics, Vol. 41, Issue 1, p. 98-107
A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007
- Kimchi-Sarfaty, C.; Oh, J. M.; Kim, I.-W.
- Science, Vol. 315, Issue 5811, p. 525-528
Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002
- Kisselev, Lev
- Structure, Vol. 10, Issue 1, p. 8-9
Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006
- Nackley, A. G.; Shabalina, S. A.; Tchivileva, I. E.
- Science, Vol. 314, Issue 5807, p. 1930-1933
Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast
journal, June 2009
- Reijns, Martin A. M.; Auchynnikava, Tatsiana; Beggs, Jean D.
- FEBS Journal, Vol. 276, Issue 13, p. 3602-3617
Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007
- Sauna, Z. E.; Kimchi-Sarfaty, C.; Ambudkar, S. V.
- Cancer Research, Vol. 67, Issue 20, p. 9609-9612
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001
- Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J.
- Journal of Bacteriology, Vol. 183, Issue 8, p. 2405-2410
Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes
journal, November 2007
- Spiller, M. P.; Reijns, M. A. M.; Beggs, J. D.
- Journal of Cell Science, Vol. 120, Issue 24
Prediction of protein function from protein sequence and structure
journal, January 1999
- Whisstock, James C.; Lesk, Arthur M.
- Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995
- Wishart, Matthew J.; Denu, John M.; Williams, John A.
- Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999
- Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
- Biochemistry, Vol. 38, Issue 36, p. 11643-11650
Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007
- Almeida, Joao; Modig, Tobias; Petersson, Anneli
- Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
Enhancement of Acid Tolerance in Zymomonas mobilis by a Proton-Buffering Peptide
journal, January 2006
- Baumler, David J.; Hung, Kai F.; Bose, Jeffrey L.
- Applied Biochemistry and Biotechnology, Vol. 134, Issue 1
Cellular Response of Shewanella oneidensis to Strontium Stress
journal, January 2006
- Brown, S. D.; Martin, M.; Deshpande, S.
- Applied and Environmental Microbiology, Vol. 72, Issue 1
Quinoprotein alcohol dehydrogenase is involved in catabolic acetate production, while NAD-dependent alcohol dehydrogenase in ethanol assimilation in Acetobacter pasteurianus SKU1108
journal, January 2003
- Chinnawirotpisan, Piyawan; Theeragool, Gunjana; Limtong, Savitree
- Journal of Bioscience and Bioengineering, Vol. 96, Issue 6, p. 564-571
Cloning of genes responsible for acetic acid resistance in Acetobacter aceti.
journal, April 1990
- Fukaya, M.; Takemura, H.; Okumura, H.
- Journal of Bacteriology, Vol. 172, Issue 4
The aarC gene responsible for acetic acid assimilation confers acetic acid resistance on acetobacter aceti
journal, January 1993
- Fukaya, Masahiro; Takemura, Hiroshi; Tayama, Kenji
- Journal of Fermentation and Bioengineering, Vol. 76, Issue 4, p. 270-275
Hfq Modulates the E-Mediated Envelope Stress Response and the 32-Mediated Cytoplasmic Stress Response in Escherichia coli
journal, December 2006
- Guisbert, E.; Rhodius, V. A.; Ahuja, N.
- Journal of Bacteriology, Vol. 189, Issue 5
Characterization of a High-Productivity Recombinant Strain of Zymomonas mobilis for Ethanol Production from Glucose/Xylose Mixtures
journal, January 2000
- Joachimsthal, Eva L.; Rogers, Peter L.
- Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
Suicide plasmids containing promoterless reporter genes can simultaneously disrupt and create fusions to target genes of diverse bacteria
journal, March 1997
- Kalogeraki, Virginia S.; Winans, Stephen C.
- Gene, Vol. 188, Issue 1, p. 69-75
A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site
journal, July 2001
- Koch, Birgit; Jensen, Linda Elise; Nybroe, Ole
- Journal of Microbiological Methods, Vol. 45, Issue 3, p. 187-195
Effects of pH and acetic acid on glucose and xylose metabolism by a genetically engineered ethanologenicEscherichia coli
journal, September 1993
- Lawford, Hugh G.; Rousseau, Joyce D.
- Applied Biochemistry and Biotechnology, Vol. 39-40, Issue 1
Improving fermentation performance of recombinant zymomonas in acetic acid-containing media
journal, March 1998
- Lawford, Hugh G.; Rousseau, Joyce D.
- Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1
Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate
journal, February 2004
- Mohagheghi, Ali; Dowe, Nancy; Schell, Daniel
- Biotechnology Letters, Vol. 26, Issue 4
A Specialized Citric Acid Cycle Requiring Succinyl-Coenzyme A (CoA):Acetate CoA-Transferase (AarC) Confers Acetic Acid Resistance on the Acidophile Acetobacter aceti
journal, May 2008
- Mullins, E. A.; Francois, J. A.; Kappock, T. J.
- Journal of Bacteriology, Vol. 190, Issue 14
Biochemical characteristics of spontaneous mutants of Acetobacter aceti deficient in ethanol oxidation.
journal, January 1985
- Okumura, Hajime; Uozumi, Takeshi; Beppu, Teruhiko
- Agricultural and Biological Chemistry, Vol. 49, Issue 8
A General System for Studying Protein−Protein Interactions in Gram-Negative Bacteria
journal, August 2008
- Pelletier, Dale A.; Hurst, Gregory B.; Foote, Linda J.
- Journal of Proteome Research, Vol. 7, Issue 8
Overexpression of the ATP-dependent helicase RecG improves resistance to weak organic acids in Escherichia coli
journal, December 2003
- Steiner, P.; Sauer, U.
- Applied Microbiology and Biotechnology, Vol. 63, Issue 3
Novel insertion sequence IS1380 from Acetobacter pasteurianus is involved in loss of ethanol-oxidizing ability.
journal, November 1991
- Takemura, H.; Horinouchi, S.; Beppu, T.
- Journal of Bacteriology, Vol. 173, Issue 22
Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12
journal, July 1994
- Tsui, Ho-Ching Tiffany; Leung, Hon-Chiu Eastwood; Winkler, Malcolm E.
- Molecular Microbiology, Vol. 13, Issue 1
The bacterial Sm-like protein Hfq: a key player in RNA transactions
journal, February 2004
- Valentin-Hansen, Poul; Eriksen, Maiken; Udesen, Christina
- Molecular Microbiology, Vol. 51, Issue 6, p. 1525-1533
The Sm-like Hfq Protein Increases OxyS RNA Interaction with Target mRNAs
journal, January 2002
- Zhang, Aixia; Wassarman, Karen M.; Ortega, Joaquin
- Molecular Cell, Vol. 9, Issue 1, p. 11-22
Global analysis of small RNA and mRNA targets of Hfq: Global analysis of Hfq targets
journal, October 2003
- Zhang, Aixia; Wassarman, Karen M.; Rosenow, Carsten
- Molecular Microbiology, Vol. 50, Issue 4
Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
journal, January 2009
- Yang, Shihui; Tschaplinski, Timothy J.; Engle, Nancy L.
- BMC Genomics, Vol. 10, Issue 1