skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microorganisms having enhanced tolerance to inhibitors and stress

Abstract

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.

Inventors:
;
Issue Date:
Research Org.:
ORNL (Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States))
Sponsoring Org.:
USDOE
OSTI Identifier:
1149918
Patent Number(s):
8,790,902
Application Number:
13/589,709
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Brown, Steven D., and Yang, Shihui. Microorganisms having enhanced tolerance to inhibitors and stress. United States: N. p., 2014. Web.
Brown, Steven D., & Yang, Shihui. Microorganisms having enhanced tolerance to inhibitors and stress. United States.
Brown, Steven D., and Yang, Shihui. Tue . "Microorganisms having enhanced tolerance to inhibitors and stress". United States. https://www.osti.gov/servlets/purl/1149918.
@article{osti_1149918,
title = {Microorganisms having enhanced tolerance to inhibitors and stress},
author = {Brown, Steven D. and Yang, Shihui},
abstractNote = {The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {7}
}

Patent:

Save / Share:

Works referenced in this record:

Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation
journal, December 2006

  • Tanaka, Fumiko; Ando, Akira; Nakamura, Toshihide
  • Food Microbiology, Vol. 23, Issue 8, p. 717-728
  • DOI: 10.1016/j.fm.2006.02.003

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


Practical limits of function prediction
journal, January 2000


A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity
journal, January 2007


Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002


Human Catechol-O-Methyltransferase Haplotypes Modulate Protein Expression by Altering mRNA Secondary Structure
journal, December 2006

  • Nackley, A. G.; Shabalina, S. A.; Tchivileva, I. E.
  • Science, Vol. 314, Issue 5807, p. 1930-1933
  • DOI: 10.1126/science.1131262

Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast
journal, June 2009


Silent Polymorphisms Speak: How They Affect Pharmacogenomics and the Treatment of Cancer
journal, October 2007


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Requirements for nuclear localization of the Lsm2-8p complex and competition between nuclear and cytoplasmic Lsm complexes
journal, November 2007

  • Spiller, M. P.; Reijns, M. A. M.; Beggs, J. D.
  • Journal of Cell Science, Vol. 120, Issue 24
  • DOI: 10.1242/jcs.019943

Prediction of protein function from protein sequence and structure
journal, January 1999

  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995

  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999

  • Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
  • Biochemistry, Vol. 38, Issue 36, p. 11643-11650
  • DOI: 10.1021/bi990993h

Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007

  • Almeida, Joao; Modig, Tobias; Petersson, Anneli
  • Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
  • DOI: 10.1002/jctb.1676

Enhancement of Acid Tolerance in Zymomonas mobilis by a Proton-Buffering Peptide
journal, January 2006

  • Baumler, David J.; Hung, Kai F.; Bose, Jeffrey L.
  • Applied Biochemistry and Biotechnology, Vol. 134, Issue 1
  • DOI: 10.1385/ABAB:134:1:15

Cellular Response of Shewanella oneidensis to Strontium Stress
journal, January 2006


Quinoprotein alcohol dehydrogenase is involved in catabolic acetate production, while NAD-dependent alcohol dehydrogenase in ethanol assimilation in Acetobacter pasteurianus SKU1108
journal, January 2003

  • Chinnawirotpisan, Piyawan; Theeragool, Gunjana; Limtong, Savitree
  • Journal of Bioscience and Bioengineering, Vol. 96, Issue 6, p. 564-571
  • DOI: 10.1016/S1389-1723(04)70150-4

Cloning of genes responsible for acetic acid resistance in Acetobacter aceti.
journal, April 1990


The aarC gene responsible for acetic acid assimilation confers acetic acid resistance on acetobacter aceti
journal, January 1993

  • Fukaya, Masahiro; Takemura, Hiroshi; Tayama, Kenji
  • Journal of Fermentation and Bioengineering, Vol. 76, Issue 4, p. 270-275
  • DOI: 10.1016/0922-338X(93)90192-B

Hfq Modulates the  E-Mediated Envelope Stress Response and the  32-Mediated Cytoplasmic Stress Response in Escherichia coli
journal, December 2006

  • Guisbert, E.; Rhodius, V. A.; Ahuja, N.
  • Journal of Bacteriology, Vol. 189, Issue 5
  • DOI: 10.1128/JB.01243-06

Characterization of a High-Productivity Recombinant Strain of Zymomonas mobilis for Ethanol Production from Glucose/Xylose Mixtures
journal, January 2000

  • Joachimsthal, Eva L.; Rogers, Peter L.
  • Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
  • DOI: 10.1385/ABAB:84-86:1-9:343

A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site
journal, July 2001

  • Koch, Birgit; Jensen, Linda Elise; Nybroe, Ole
  • Journal of Microbiological Methods, Vol. 45, Issue 3, p. 187-195
  • DOI: 10.1016/S0167-7012(01)00246-9

Effects of pH and acetic acid on glucose and xylose metabolism by a genetically engineered ethanologenicEscherichia coli
journal, September 1993

  • Lawford, Hugh G.; Rousseau, Joyce D.
  • Applied Biochemistry and Biotechnology, Vol. 39-40, Issue 1
  • DOI: 10.1007/BF02918999

Improving fermentation performance of recombinant zymomonas in acetic acid-containing media
journal, March 1998

  • Lawford, Hugh G.; Rousseau, Joyce D.
  • Applied Biochemistry and Biotechnology, Vol. 70-72, Issue 1
  • DOI: 10.1007/BF02920133

Performance of a newly developed integrant of Zymomonas mobilis for ethanol production on corn stover hydrolysate
journal, February 2004


Biochemical characteristics of spontaneous mutants of Acetobacter aceti deficient in ethanol oxidation.
journal, January 1985

  • Okumura, Hajime; Uozumi, Takeshi; Beppu, Teruhiko
  • Agricultural and Biological Chemistry, Vol. 49, Issue 8
  • DOI: 10.1271/bbb1961.49.2485

A General System for Studying Protein−Protein Interactions in Gram-Negative Bacteria
journal, August 2008

  • Pelletier, Dale A.; Hurst, Gregory B.; Foote, Linda J.
  • Journal of Proteome Research, Vol. 7, Issue 8
  • DOI: 10.1021/pr8001832

Overexpression of the ATP-dependent helicase RecG improves resistance to weak organic acids in Escherichia coli
journal, December 2003


Novel insertion sequence IS1380 from Acetobacter pasteurianus is involved in loss of ethanol-oxidizing ability.
journal, November 1991


Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12
journal, July 1994


The bacterial Sm-like protein Hfq: a key player in RNA transactions
journal, February 2004


The Sm-like Hfq Protein Increases OxyS RNA Interaction with Target mRNAs
journal, January 2002


Global analysis of small RNA and mRNA targets of Hfq: Global analysis of Hfq targets
journal, October 2003


Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations
journal, January 2009

  • Yang, Shihui; Tschaplinski, Timothy J.; Engle, Nancy L.
  • BMC Genomics, Vol. 10, Issue 1
  • DOI: 10.1186/1471-2164-10-34