Global to push GA events into
skip to main content

Title: Microorganisms having enhanced tolerance to inhibitors and stress

The present invention provides genetically modified strains of microorganisms that display enhanced tolerance to stress and/or inhibitors such as sodium acetate and vanillin. The enhanced tolerance can be achieved by increasing the expression of a protein of the Sm-like superfamily such as a bacterial Hfq protein and a fungal Sm or Lsm protein. Further, the present invention provides methods of producing alcohol from biomass materials by using the genetically modified microorganisms of the present invention.
Inventors:
;
Issue Date:
OSTI Identifier:
1149918
Assignee:
UT-Battelle, LLC (Oak Ridge, TN) ORNL
Patent Number(s):
8,790,902
Application Number:
13/589,709
Contract Number:
AC05-00OR22725
Research Org:
ORNL (Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States))
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Functional genomic analysis of commercial baker's yeast during initial stages of model dough-fermentation
journal, December 2006
  • Tanaka, Fumiko; Ando, Akira; Nakamura, Toshihide
  • Food Microbiology, Vol. 23, Issue 8, p. 717-728
  • DOI: 10.1016/j.fm.2006.02.003

Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998

Practical limits of function prediction
journal, January 2000

Polypeptide Release Factors in Prokaryotes and Eukaryotes
journal, January 2002

Analysis of Lsm1p and Lsm8p domains in the cellular localization of Lsm complexes in budding yeast
journal, June 2009

Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001

Prediction of protein function from protein sequence and structure
journal, January 1999
  • Whisstock, James C.; Lesk, Arthur M.
  • Quarterly Reviews of Biophysics, Vol. 36, Issue 3, p. 307-340
  • DOI: 10.1017/S0033583503003901

A Single Mutation Converts a Novel Phosphotyrosine Binding Domain into a Dual-specificity Phosphatase
journal, November 1995
  • Wishart, Matthew J.; Denu, John M.; Williams, John A.
  • Journal of Biological Chemistry, Vol. 270, Issue 45, p. 26782-26785
  • DOI: 10.1074/jbc.270.45.26782

Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999
  • Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
  • Biochemistry, Vol. 38, Issue 36, p. 11643-11650
  • DOI: 10.1021/bi990993h

Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae
journal, January 2007
  • Almeida, Joao; Modig, Tobias; Petersson, Anneli
  • Journal of Chemical Technology & Biotechnology, Vol. 82, Issue 4, p. 340-349
  • DOI: 10.1002/jctb.1676

Quinoprotein alcohol dehydrogenase is involved in catabolic acetate production, while NAD-dependent alcohol dehydrogenase in ethanol assimilation in Acetobacter pasteurianus SKU1108
journal, January 2003
  • Chinnawirotpisan, Piyawan; Theeragool, Gunjana; Limtong, Savitree
  • Journal of Bioscience and Bioengineering, Vol. 96, Issue 6, p. 564-571
  • DOI: 10.1016/S1389-1723(04)70150-4

The aarC gene responsible for acetic acid assimilation confers acetic acid resistance on acetobacter aceti
journal, January 1993
  • Fukaya, Masahiro; Takemura, Hiroshi; Tayama, Kenji
  • Journal of Fermentation and Bioengineering, Vol. 76, Issue 4, p. 270-275
  • DOI: 10.1016/0922-338X(93)90192-B

A panel of Tn7-based vectors for insertion of the gfp marker gene or for delivery of cloned DNA into Gram-negative bacteria at a neutral chromosomal site
journal, July 2001
  • Koch, Birgit; Jensen, Linda Elise; Nybroe, Ole
  • Journal of Microbiological Methods, Vol. 45, Issue 3, p. 187-195
  • DOI: 10.1016/S0167-7012(01)00246-9

The bacterial Sm-like protein Hfq: a key player in RNA transactions
journal, February 2004

The Sm-like Hfq Protein Increases OxyS RNA Interaction with Target mRNAs
journal, January 2002