Title: Extracellular secretion of recombinant proteins

Abstract

Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.

Inventors:

Linger, Jeffrey G.; Darzins, Aldis

Issue Date:

2014-07-22

Research Org.:

National Energy Technology Laboratory (NETL), Pittsburgh, PA, Morgantown, WV (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1149687

Patent Number(s):

8785159

Application Number:

12/950,442

Assignee:

Alliance for Sustainable Energy, LLC (Golden, CO)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/2437 - {Cellulases

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/036 - targeting to the medium outside of the cell, e.g. type III secretion

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/22 - Processes using, or culture media containing, cellulose or hydrolysates thereof

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/02 - Monosaccharides

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K14/195 - from bacteria

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/14 - produced by the action of a carbohydrase {

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/034 - containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01004 - Cellulase

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DOE Contract Number:  

AC36-08G028308

Resource Type:

Patent

Resource Relation:

Patent File Date: 2010 Nov 19

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY