Title: Toxicology and cellular effect of manufactured nanomaterials

Abstract

The increasing use of nanotechnology in consumer products and medical applications underlies the importance of understanding its potential toxic effects to people and the environment. Herein are described methods and assays to predict and evaluate the cellular effects of nanomaterial exposure. Exposing cells to nanomaterials at cytotoxic doses induces cell cycle arrest and increases apoptosis/necrosis, activates genes involved in cellular transport, metabolism, cell cycle regulation, and stress response. Certain nanomaterials induce genes indicative of a strong immune and inflammatory response within skin fibroblasts. Furthermore, the described multiwall carbon nanoonions (MWCNOs) can be used as a therapeutic in the treatment of cancer due to its cytotoxicity.

Inventors:

Chen, Fanqing

Issue Date:

2014-07-22

Research Org.:

Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1149613

Patent Number(s):

8785505

Application Number:

12/111,026

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K47/6855 - {the tumour determinant being from breast cancer cell}
A61K47/6925 - {the form being a microcapsule, nanocapsule, microbubble or nanobubble}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y5/00 - Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6883 - for diseases caused by alterations of genetic material
C12Q2600/106 - Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
C12Q2600/142 - Toxicological screening, e.g. expression profiles which identify toxicity
C12Q2600/158 - Expression markers

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/5014 - {for testing toxicity}

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DOE Contract Number:  

W-7405-Eng-48; AC05-CH11231

Resource Type:

Patent

Resource Relation:

Patent File Date: 2008 Apr 28

Country of Publication:

United States

Language:

English

Subject:

77 NANOSCIENCE AND NANOTECHNOLOGY