Title: Methods for making nucleotide probes for sequencing and synthesis

Abstract

Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.

Inventors:

Church, George M; Zhang, Kun; Chou, Joseph

Issue Date:

2014-07-08

Research Org.:

Harvard College, Cambridge, MA (USA).

Sponsoring Org.:

USDOE

OSTI Identifier:

1140127

Patent Number(s):

8771950

Application Number:

12/187,460

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q2537/143 - Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
C12Q2535/113 - Cycle sequencing
C12Q2565/543 - characterised by the use of two or more capture oligonucleotide primers in concert, e.g. bridge amplification
C12Q1/6813 - Hybridisation assays
C12Q1/6837 - using probe arrays or probe chips

Show less

DOE Contract Number:  

FG02-02ER63445

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES