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Title: System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source

A system and method for analyzing a particle in a sample stream of a flow cytometer or the like. The system has a light source, such as a laser pointer module, for generating a low powered light beam and a fluidics apparatus which is configured to transport particles in the sample stream at substantially low velocity through the light beam for interrogation. Detectors, such as photomultiplier tubes, are configured to detect optical signals generated in response to the light beam impinging the particles. Signal conditioning circuitry is connected to each of the detectors to condition each detector output into electronic signals for processing and is designed to have a limited frequency response to filter high frequency noise from the detector output signals.
Inventors:
;
Issue Date:
OSTI Identifier:
1136413
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM) LANL
Patent Number(s):
8,767,208
Application Number:
13/966,624
Contract Number:
AC52-06NA25396
Research Org:
LANL (Los Alamos National Laboratory (LANL), Los Alamos, NM (United States))
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

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journal, February 2004
  • Morgan, J.; Spengler, J. F.; Kuznetsova, L.
  • Toxicology in Vitro, Vol. 18, Issue 1, p. 115-120
  • DOI: 10.1016/j.tiv.2003.08.004

The Small-Angle Light Scattering of Biological Cells: Theoretical Considerations
journal, August 1970

Acoustic control of suspended particles in micro fluidic chips
journal, January 2004
  • Nilsson, Andreas; Petersson, Filip; Jönsson, Henrik
  • Lab Chip, Vol. 4, Issue 2, p. 131-135
  • DOI: 10.1039/B313493H

Layered piezoelectric resonators with an arbitrary number of electrodes (general one‐dimensional treatment)
journal, September 1991
  • Nowotny, Helmut; Benes, Ewald; Schmid, Michael
  • The Journal of the Acoustical Society of America, Vol. 90, Issue 3, p. 1238-1245
  • DOI: 10.1121/1.401915

Acoustically aided separation of oil droplets from aqueous emulsions
journal, August 2004
  • Pangu, Gautam D.; Feke, Donald L.
  • Chemical Engineering Science, Vol. 59, Issue 15, p. 3183-3193
  • DOI: 10.1016/j.ces.2004.03.038

Separation of lipids from blood utilizing ultrasonic standing waves in microfluidic channels
journal, January 2004
  • Petersson, Filip; Nilsson, Andreas; Holm, Cecilia
  • The Analyst, Vol. 129, Issue 10, p. 938-943
  • DOI: 10.1039/B409139F

Carrier Medium Exchange through Ultrasonic Particle Switching in Microfluidic Channels
journal, March 2005
  • Petersson, Filip; Nilsson, Andreas; Jönsson, Henrik
  • Analytical Chemistry, Vol. 77, Issue 5, p. 1216-1221
  • DOI: 10.1021/ac048394q

Free Flow Acoustophoresis:  Microfluidic-Based Mode of Particle and Cell Separation
journal, July 2007
  • Petersson, Filip; Åberg, Lena; Swärd-Nilsson, Ann-Margret
  • Analytical Chemistry, Vol. 79, Issue 14, p. 5117-5123
  • DOI: 10.1021/ac070444e

Multifunctional Encoded Particles for High-Throughput Biomolecule Analysis
journal, March 2007
  • Pregibon, Daniel C.; Toner, Mehmet; Doyle, Patrick S.
  • Science, Vol. 315, Issue 5817, p. 1393-1396
  • DOI: 10.1126/science.1134929

Evaluation of SDF-1/CXCR4-induced Ca2+ signaling by fluorometric imaging plate reader (FLIPR) and flow cytometry
journal, December 2002
  • Princen, Katrien; Hatse, Sigrid; Vermeire, Kurt
  • Cytometry, Vol. 51A, Issue 1, p. 35-45
  • DOI: 10.1002/cyto.a.10008

Monodisperse Mesoporous Silica Microspheres Formed by Evaporation-Induced Self Assembly of Surfactant Templates in Aerosols
journal, September 2002

A novel nozzle for more efficient sperm orientation to improve sorting efficiency of X and Y chromosome-bearing sperm
journal, December 1998

A numerically stable global matrix method for cylindrically layered shells excited by ring forces
journal, June 1994
  • Ricks, David C.; Schmidt, Henrik
  • The Journal of the Acoustical Society of America, Vol. 95, Issue 6, p. 3339-3349
  • DOI: 10.1121/1.409953

Oscillational instabilities in single‐mode acoustic levitators
journal, January 1990
  • Rudnick, Joseph; Barmatz, M.
  • The Journal of the Acoustical Society of America, Vol. 87, Issue 1, p. 81-92
  • DOI: 10.1121/1.398916

Ultrasonic manipulation of locomotive microorganisms and evaluation of their activity
journal, December 2002
  • Saito, Mitsunori; Kitamura, Norio; Terauchi, Masaki
  • Journal of Applied Physics, Vol. 92, Issue 12, p. 7581-7586
  • DOI: 10.1063/1.1522813

Ultrasonic trapping of paramecia and estimation of their locomotive force
journal, October 1997
  • Saito, Mitsunori; Izumida, Shin-ya; Hirota, Jun
  • Applied Physics Letters, Vol. 71, Issue 14, p. 1909-1911
  • DOI: 10.1063/1.120436

Ultrasonic waves for fabricating lattice structure in composite materials
conference, September 1999
  • Saito, Mitsunori; Itagaki, Kazuhiro; Imanishi, Yoshihiro
  • SPIE's International Symposium on Optical Science, Engineering, and Instrumentation
  • DOI: 10.1117/12.363793

Continuous Flow Microfluidic Device for Rapid Erythrocyte Lysis
journal, November 2004
  • Sethu, Palaniappan; Anahtar, Melis; Moldawer, Lyle L.
  • Analytical Chemistry, Vol. 76, Issue 21, p. 6247-6253
  • DOI: 10.1021/ac049429p

Individual Escherichia coli cells studied from light scattering with the scanning flow cytometer
journal, January 2000

Ultrasonic four‐wave mixing mediated by an aqueous suspension of microspheres: Theoretical steady‐state properties
journal, September 1995
  • Simpson, Harry J.; Marston, Philip L.
  • The Journal of the Acoustical Society of America, Vol. 98, Issue 3, p. 1731-1741
  • DOI: 10.1121/1.413437

New types of microspheres and microsphere-related materials for medical diagnostics
journal, October 2002
  • Slomkowski, Stanislaw; Basinska, Teresa; Miksa, Beata
  • Polymers for Advanced Technologies, Vol. 13, Issue 10-12, p. 905-918
  • DOI: 10.1002/pat.283

A differential amplifier circuit for reducing noise in axial light loss measurements
journal, July 1983

Dual-laser, differential fluorescence correction method for reducing cellular background autofluorescence
journal, November 1986

Enhanced immunofluorescence measurement resolution of surface antigens on highly autofluorescent, glutaraldehyde-fixed cells analyzed by phase-sensitive flow cytometry
journal, December 1999

Resolving leukocytes using axial light loss
journal, July 1989
  • Stewart, Carleton C.; Stewart, Sigrid J.; Habbersett, Robert C.
  • Cytometry, Vol. 10, Issue 4, p. 426-432
  • DOI: 10.1002/cyto.990100410

Data Analysis for a Dual-Channel Virus Counter
journal, April 2005
  • Stoffel, Carrie L.; Rowlen, Kathy L.
  • Analytical Chemistry, Vol. 77, Issue 7, p. 2243-2246
  • DOI: 10.1021/ac048626l

Design and characterization of a compact dual channel virus counter
journal, January 2005
  • Stoffel, Carrie L.; Kathy, Robin Finch; Rowlen, Kathy L.
  • Cytometry Part A, Vol. 65A, Issue 2, p. 140-147
  • DOI: 10.1002/cyto.a.20145

A means for orienting flat cells in flow systems
journal, July 1978

Separation devices based on forced coincidence response of fluid‐filled pipes
journal, June 1992
  • Tolt, Thomas L.; Feke, Donald L.
  • The Journal of the Acoustical Society of America, Vol. 91, Issue 6, p. 3152-3156
  • DOI: 10.1121/1.402853

Separation of dispersed phases from liquids in acoustically driven chambers
journal, February 1993

Modelling of particle paths passing through an ultrasonic standing wave
journal, April 2004

Experimental study of streaming flows associated with ultrasonic levitators
journal, November 1994
  • Trinh, E. H.; Robey, J. L.
  • Physics of Fluids, Vol. 6, Issue 11, p. 3567-3579
  • DOI: 10.1063/1.868415

Compact acoustic levitation device for studies in fluid dynamics and material science in the laboratory and microgravity
journal, November 1985
  • Trinh, E. H.
  • Review of Scientific Instruments, Vol. 56, Issue 11, p. 2059-2065
  • DOI: 10.1063/1.1138419

Trapping of heavy gases in stationary ultrasonic fields
journal, September 2002
  • Tuckermann, Rudolf; Neidhart, Bernd; Lierke, Ernst G.
  • Chemical Physics Letters, Vol. 363, Issue 3-4, p. 349-354
  • DOI: 10.1016/S0009-2614(02)01178-8

Ruthenium(II) complex with a notably long excited state lifetime
journal, January 2000
  • Tyson, Daniel S.; Bialecki, Jason; Castellano, Felix N.
  • Chemical Communications, Vol. 0, Issue 23, p. 2355-2356
  • DOI: 10.1039/B007336I

On the drift of aerosol particles in sonic fields
journal, September 1992

Strategy for selection of methods for separation of bioparticles from particle mixtures
journal, January 2006
  • van Hee, P.; Hoeben, M. A.; van der Lans, R. G. J. M.
  • Biotechnology and Bioengineering, Vol. 94, Issue 4, p. 689-709
  • DOI: 10.1002/bit.20885

Retention and Viability Characteristics of Mammalian Cells in an Acoustically Driven Polymer Mesh
journal, January 2004
  • Wang, Zhaowei; Grabenstetter, Paul; Feke, Donald L.
  • Biotechnology Progress, Vol. 20, Issue 1, p. 384-387
  • DOI: 10.1021/bp034105s

Extension of acoustic levitation to include the study of micron‐size particles in a more compressible host liquid
journal, May 1982
  • Weiser, Mary Ann H.; Apfel, Robert E.
  • The Journal of the Acoustical Society of America, Vol. 71, Issue 5, p. 1261-1268
  • DOI: 10.1121/1.387776

Particle column formation in a stationary ultrasonic field
journal, January 1992
  • Whitworth, Glenn; Coakley, W. T.
  • The Journal of the Acoustical Society of America, Vol. 91, Issue 1, p. 79-85
  • DOI: 10.1121/1.402622

Transport and harvesting of suspended particles using modulated ultrasound
journal, November 1991

Diazo Coupling Method for Covalent Attachment of Proteins to Solid Substrates
journal, March 2006
  • Wu, Yang; Buranda, Tione; Metzenberg, Robert L.
  • Bioconjugate Chemistry, Vol. 17, Issue 2, p. 359-365
  • DOI: 10.1021/bc050278m

Flow cytometry to evaluate Theileria sergenti parasitemia using the fluorescent nucleic acid stain, SYTO16
journal, January 2000

Micro particle trapping by opposite phases ultrasonic travelling waves
journal, July 1998

Deoxyribonucleic acid concentration using acoustic radiation force
journal, February 1996
  • Yasuda, Kenji; Kiyama, Masaharu; Umemura, Shin‐ichiro
  • The Journal of the Acoustical Society of America, Vol. 99, Issue 2, p. 1248-1251
  • DOI: 10.1121/1.414635

Non-destructive, non-contact handling method for biomaterials in micro-chamber by ultrasound
journal, June 2000

Particle separation using acoustic radiation force and elecrostatic force
journal, April 1996
  • Yasuda, Kenji; Umemura, Shin‐ichiro; Takeda, Kazuo
  • The Journal of the Acoustical Society of America, Vol. 99, Issue 4, p. 1965-1970
  • DOI: 10.1121/1.415382

Using acoustic radiation force as a concentration method for erythrocytes
journal, July 1997
  • Yasuda, Kenji; Haupt, Stephan Shuichi; Umemura, Shin-ichiro
  • The Journal of the Acoustical Society of America, Vol. 102, Issue 1, p. 642-645
  • DOI: 10.1121/1.421009

Preparation of three terbium complexes with p-aminobenzoic acid and investigation of crystal structure influence on luminescence property
journal, October 2004
  • Ye, Chao-Hong; Sun, Hao-Ling; Wang, Xin-Yi
  • Journal of Solid State Chemistry, Vol. 177, Issue 10, p. 3735-3742
  • DOI: 10.1016/j.jssc.2004.06.038

Lanthanide-based luminescence probes and time-resolved luminescence bioassays
journal, May 2006

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